Fate of fluoroquinolones in field soil environment after
incorporation of poultry litter from a farm with enrofloxacin
administration via drinking water

J 2024

Fate of fluoroquinolones in field soil environment after incorporation of poultry litter from a farm with enrofloxacin administration via drinking water

FUCIK, Jan, Anna AMRICHOVA, Kristyna BRABCOVA, Renata KARPÍŠKOVÁ, Ivana KOLÁČKOVÁ et. al.

Basic information

Original name

Fate of fluoroquinolones in field soil environment after incorporation of poultry litter from a farm with enrofloxacin administration via drinking water

Authors

FUCIK, Jan (203 Czech Republic), Anna AMRICHOVA (203 Czech Republic), Kristyna BRABCOVA (203 Czech Republic), Renata KARPÍŠKOVÁ (203 Czech Republic, belonging to the institution), Ivana KOLÁČKOVÁ (203 Czech Republic, belonging to the institution), Lucie POKLUDOVA (203 Czech Republic), Sarka POLAKOVA (203 Czech Republic) and Ludmila MRAVCOVA (203 Czech Republic)

Edition

Environmental Science and Pollution Research, HEIDELBERG, Springer, 2024, 0944-1344

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30304 Public and environmental health

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 5.800 in 2022

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.1007/s11356-024-32492-x

UT WoS

001163799600009

Keywords in English

Veterinary antimicrobials; Fluoroquinolones; Manure fertilization; Antimicrobial resistance; Solid phase extraction; Liquid chromatography; Mass spectrometry; PCR

Abstract

V originále

The practice of incorporating animal manure into soil is supported within the European Circular economy as a possible substitute for mineral fertilizers and will become crucial for the sustainability of agriculture. However, this practice may indirectly contribute to the dissemination of antibiotics, resistance bacteria, and resistance genes. In this study, medicated drinking water and poultry litter samples were obtained from a broiler-chick farm. The obtained poultry litter was incorporated into the soil at the experimental field site. The objectives of this research project were first to develop analytical methods able to quantify fluoroquinolones (FQs) in medicated drinking water, poultry litter, and soil samples by LC-MS; second to study the fate of these FQs in the soil environment after incorporation of poultry litter from flock medicated by enrofloxacin (ENR); and third to screen the occurrence of selected fluoroquinolone resistance encoding genes in poultry litter and soil samples (PCR analysis). FQs were quantified in the broiler farm's medicated drinking water (41.0 +/- 0.3 mg center dot L-1 of ENR) and poultry litter (up to 70 mg center dot kg-1 of FQs). The persistence of FQs in the soil environment over 112 days was monitored and evaluated (ENR concentrations ranged from 36 mu g center dot kg-1 to 9 mu g center dot kg-1 after 100 days). The presence of resistance genes was confirmed in both poultry litter and soil samples, in agreement with the risk assessment for the selection of AMR in soil based on ENR concentrations. This work provides a new, comprehensive perspective on the entry and long-term fate of antimicrobials in the terrestrial environment and their consequences after the incorporation of poultry litter into agricultural fields.

Citovat

FUCIK, Jan, Anna AMRICHOVA, Kristyna BRABCOVA, Renata KARPÍŠKOVÁ, Ivana KOLÁČKOVÁ, Lucie POKLUDOVA, Sarka POLAKOVA and Ludmila MRAVCOVA. Fate of fluoroquinolones in field soil environment after incorporation of poultry litter from a farm with enrofloxacin administration via drinking water. Environmental Science and Pollution Research. HEIDELBERG: Springer, 2024, vol. 31, No 13, p. 20017-20032. ISSN 0944-1344. Available from: https://dx.doi.org/10.1007/s11356-024-32492-x.

@article{2409497,
   author = {Fucik, Jan and Amrichova, Anna and Brabcova, Kristyna and Karpíšková, Renata and Koláčková, Ivana and Pokludova, Lucie and Polakova, Sarka and Mravcova, Ludmila},
   article_location = {HEIDELBERG},
   article_number = {13},
   doi = {http://dx.doi.org/10.1007/s11356-024-32492-x},
   keywords = {Veterinary antimicrobials; Fluoroquinolones; Manure fertilization; Antimicrobial resistance; Solid phase extraction; Liquid chromatography; Mass spectrometry; PCR},
   language = {eng},
   issn = {0944-1344},
   journal = {Environmental Science and Pollution Research},
   title = {Fate of fluoroquinolones in field soil environment after incorporation of poultry litter from a farm with enrofloxacin administration via drinking water},
   url = {https://link.springer.com/article/10.1007/s11356-024-32492-x},
   volume = {31},
   year = {2024}
}

TY  - JOUR
ID  - 2409497
AU  - Fucik, Jan - Amrichova, Anna - Brabcova, Kristyna - Karpíšková, Renata - Koláčková, Ivana - Pokludova, Lucie - Polakova, Sarka - Mravcova, Ludmila
PY  - 2024
TI  - Fate of fluoroquinolones in field soil environment after incorporation of poultry litter from a farm with enrofloxacin administration via drinking water
JF  - Environmental Science and Pollution Research
VL  - 31
IS  - 13
SP  - 20017-20032
EP  - 20017-20032
PB  - Springer
SN  - 09441344
KW  - Veterinary antimicrobials
KW  - Fluoroquinolones
KW  - Manure fertilization
KW  - Antimicrobial resistance
KW  - Solid phase extraction
KW  - Liquid chromatography
KW  - Mass spectrometry
KW  - PCR
UR  - https://link.springer.com/article/10.1007/s11356-024-32492-x
N2  - The practice of incorporating animal manure into soil is supported within the European Circular economy as a possible substitute for mineral fertilizers and will become crucial for the sustainability of agriculture. However, this practice may indirectly contribute to the dissemination of antibiotics, resistance bacteria, and resistance genes. In this study, medicated drinking water and poultry litter samples were obtained from a broiler-chick farm. The obtained poultry litter was incorporated into the soil at the experimental field site. The objectives of this research project were first to develop analytical methods able to quantify fluoroquinolones (FQs) in medicated drinking water, poultry litter, and soil samples by LC-MS; second to study the fate of these FQs in the soil environment after incorporation of poultry litter from flock medicated by enrofloxacin (ENR); and third to screen the occurrence of selected fluoroquinolone resistance encoding genes in poultry litter and soil samples (PCR analysis). FQs were quantified in the broiler farm's medicated drinking water (41.0 +/- 0.3 mg center dot L-1 of ENR) and poultry litter (up to 70 mg center dot kg-1 of FQs). The persistence of FQs in the soil environment over 112 days was monitored and evaluated (ENR concentrations ranged from 36 mu g center dot kg-1 to 9 mu g center dot kg-1 after 100 days). The presence of resistance genes was confirmed in both poultry litter and soil samples, in agreement with the risk assessment for the selection of AMR in soil based on ENR concentrations. This work provides a new, comprehensive perspective on the entry and long-term fate of antimicrobials in the terrestrial environment and their consequences after the incorporation of poultry litter into agricultural fields.
ER  -

FUCIK, Jan, Anna AMRICHOVA, Kristyna BRABCOVA, Renata KARPÍŠKOVÁ, Ivana KOLÁČKOVÁ, Lucie POKLUDOVA, Sarka POLAKOVA and Ludmila MRAVCOVA. Fate of fluoroquinolones in field soil environment after incorporation of poultry litter from a farm with enrofloxacin administration via drinking water. \textit{Environmental Science and Pollution Research}. HEIDELBERG: Springer, 2024, vol.~31, No~13, p.~20017-20032. ISSN~0944-1344. Available from: https://dx.doi.org/10.1007/s11356-024-32492-x.

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