SOVOVÁ, Kateřina, Petra VAŠÍČKOVÁ, Vojtěch VALÁŠEK, David VÝRAVSKÝ, Věra OČENÁŠKOVÁ, Eva JURANOVÁ, Milena BUŠOVÁ, Milan TUČEK, Vladimír BENCKO and Hana ZVĚŘINOVÁ MLEJNKOVÁ. SARS-CoV-2 wastewater surveillance in the Czech Republic: Spatial and temporal differences in SARS-CoV-2 RNA concentrations and relationship to clinical data and wastewater parameters. Water Research X. Elsevier Ltd, 2024, vol. 23, May 2024, p. 1-8. ISSN 2589-9147. Available from: https://dx.doi.org/10.1016/j.wroa.2024.100220.
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Basic information
Original name SARS-CoV-2 wastewater surveillance in the Czech Republic: Spatial and temporal differences in SARS-CoV-2 RNA concentrations and relationship to clinical data and wastewater parameters
Authors SOVOVÁ, Kateřina, Petra VAŠÍČKOVÁ (203 Czech Republic, belonging to the institution), Vojtěch VALÁŠEK, David VÝRAVSKÝ, Věra OČENÁŠKOVÁ, Eva JURANOVÁ, Milena BUŠOVÁ, Milan TUČEK, Vladimír BENCKO and Hana ZVĚŘINOVÁ MLEJNKOVÁ.
Edition Water Research X, Elsevier Ltd, 2024, 2589-9147.
Other information
Original language English
Type of outcome Article in a journal
Field of Study 10607 Virology
Country of publisher Netherlands
Confidentiality degree is not subject to a state or trade secret
WWW URL
Impact factor Impact factor: 7.500 in 2022
Organization unit Faculty of Science
Doi http://dx.doi.org/10.1016/j.wroa.2024.100220
UT WoS 001234113000001
Keywords in English Wastewater-based epidemiology; COVID-19; Virus; PCR; Wastewater treatment plant
Tags rivok
Tags International impact, Reviewed
Changed by Changed by: Mgr. Marie Šípková, DiS., učo 437722. Changed: 10/6/2024 12:52.
Abstract
This study presents the results of systematic wastewater monitoring of SARS-CoV-2 RNA and basic wastewater parameters from four different wastewater treatment plants (WWTPs) in the Czech Republic over the 2020 -2022 epidemic. Two-step reverse -transcription quantitative PCR targeting genes encoding the N and Nsp12 proteins was employed to detect SARS-CoV-2 RNA loading in 420 wastewater samples. The results obtained were used to evaluate the potential of wastewater analysis for describing the epidemiological situation in cities of different sizes and determining temporal differences based on the prevailing SARS-CoV-2 variant. Strong correlations between the number of active and hospitalised COVID-19 cases in each WWTP catchment area and the concentration of SARS-CoV-2 RNA detected in the wastewater clearly demonstrated the suitability of this wastewater -based epidemiological approach for WWTPs of different sizes and characteristics, despite differences in SARS-CoV-2 variant waves, with some WWTPs showing high predictive potential. This study demonstrated on the data from the Czech Republic that targeted systematic monitoring of wastewater provides sufficiently robust data for surveillance of viral loads in sample populations, and thus contributes to preventing the spread of infection and subsequent introduction of appropriate measures.
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