SARS-CoV-2 wastewater surveillance in the Czech Republic:
Spatial and temporal differences in SARS-CoV-2 RNA
concentrations and relationship to clinical data and wastewater
parameters

J 2024

SARS-CoV-2 wastewater surveillance in the Czech Republic: Spatial and temporal differences in SARS-CoV-2 RNA concentrations and relationship to clinical data and wastewater parameters

SOVOVÁ, Kateřina, Petra VAŠÍČKOVÁ, Vojtěch VALÁŠEK, David VÝRAVSKÝ, Věra OČENÁŠKOVÁ et. al.

Basic information

Original name

SARS-CoV-2 wastewater surveillance in the Czech Republic: Spatial and temporal differences in SARS-CoV-2 RNA concentrations and relationship to clinical data and wastewater parameters

Authors

SOVOVÁ, Kateřina, Petra VAŠÍČKOVÁ (203 Czech Republic, belonging to the institution), Vojtěch VALÁŠEK, David VÝRAVSKÝ, Věra OČENÁŠKOVÁ, Eva JURANOVÁ, Milena BUŠOVÁ, Milan TUČEK, Vladimír BENCKO and Hana ZVĚŘINOVÁ MLEJNKOVÁ

Edition

Water Research X, Elsevier Ltd, 2024, 2589-9147

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10607 Virology

Country of publisher

Netherlands

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 7.500 in 2022

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.1016/j.wroa.2024.100220

UT WoS

001234113000001

Keywords in English

Wastewater-based epidemiology; COVID-19; Virus; PCR; Wastewater treatment plant

Abstract

V originále

This study presents the results of systematic wastewater monitoring of SARS-CoV-2 RNA and basic wastewater parameters from four different wastewater treatment plants (WWTPs) in the Czech Republic over the 2020 -2022 epidemic. Two-step reverse -transcription quantitative PCR targeting genes encoding the N and Nsp12 proteins was employed to detect SARS-CoV-2 RNA loading in 420 wastewater samples. The results obtained were used to evaluate the potential of wastewater analysis for describing the epidemiological situation in cities of different sizes and determining temporal differences based on the prevailing SARS-CoV-2 variant. Strong correlations between the number of active and hospitalised COVID-19 cases in each WWTP catchment area and the concentration of SARS-CoV-2 RNA detected in the wastewater clearly demonstrated the suitability of this wastewater -based epidemiological approach for WWTPs of different sizes and characteristics, despite differences in SARS-CoV-2 variant waves, with some WWTPs showing high predictive potential. This study demonstrated on the data from the Czech Republic that targeted systematic monitoring of wastewater provides sufficiently robust data for surveillance of viral loads in sample populations, and thus contributes to preventing the spread of infection and subsequent introduction of appropriate measures.

Citovat

SOVOVÁ, Kateřina, Petra VAŠÍČKOVÁ, Vojtěch VALÁŠEK, David VÝRAVSKÝ, Věra OČENÁŠKOVÁ, Eva JURANOVÁ, Milena BUŠOVÁ, Milan TUČEK, Vladimír BENCKO and Hana ZVĚŘINOVÁ MLEJNKOVÁ. SARS-CoV-2 wastewater surveillance in the Czech Republic: Spatial and temporal differences in SARS-CoV-2 RNA concentrations and relationship to clinical data and wastewater parameters. Water Research X. Elsevier Ltd, 2024, vol. 23, May 2024, p. 1-8. ISSN 2589-9147. Available from: https://dx.doi.org/10.1016/j.wroa.2024.100220.

@article{2409501,
   author = {Sovová, Kateřina and Vašíčková, Petra and Valášek, Vojtěch and Výravský, David and Očenášková, Věra and Juranová, Eva and Bušová, Milena and Tuček, Milan and Bencko, Vladimír and Zvěřinová Mlejnková, Hana},
   article_number = {May 2024},
   doi = {http://dx.doi.org/10.1016/j.wroa.2024.100220},
   keywords = {Wastewater-based epidemiology; COVID-19; Virus; PCR; Wastewater treatment plant},
   language = {eng},
   issn = {2589-9147},
   journal = {Water Research X},
   title = {SARS-CoV-2 wastewater surveillance in the Czech Republic: Spatial and temporal differences in SARS-CoV-2 RNA concentrations and relationship to clinical data and wastewater parameters},
   url = {https://www.sciencedirect.com/science/article/pii/S2589914724000100},
   volume = {23},
   year = {2024}
}

TY  - JOUR
ID  - 2409501
AU  - Sovová, Kateřina - Vašíčková, Petra - Valášek, Vojtěch - Výravský, David - Očenášková, Věra - Juranová, Eva - Bušová, Milena - Tuček, Milan - Bencko, Vladimír - Zvěřinová Mlejnková, Hana
PY  - 2024
TI  - SARS-CoV-2 wastewater surveillance in the Czech Republic: Spatial and temporal differences in SARS-CoV-2 RNA concentrations and relationship to clinical data and wastewater parameters
JF  - Water Research X
VL  - 23
IS  - May 2024
SP  - 1-8
EP  - 1-8
PB  - Elsevier Ltd
SN  - 25899147
KW  - Wastewater-based epidemiology
KW  - COVID-19
KW  - Virus
KW  - PCR
KW  - Wastewater treatment plant
UR  - https://www.sciencedirect.com/science/article/pii/S2589914724000100
N2  - This study presents the results of systematic wastewater monitoring of SARS-CoV-2 RNA and basic wastewater parameters from four different wastewater treatment plants (WWTPs) in the Czech Republic over the 2020 -2022 epidemic. Two-step reverse -transcription quantitative PCR targeting genes encoding the N and Nsp12 proteins was employed to detect SARS-CoV-2 RNA loading in 420 wastewater samples. The results obtained were used to evaluate the potential of wastewater analysis for describing the epidemiological situation in cities of different sizes and determining temporal differences based on the prevailing SARS-CoV-2 variant. Strong correlations between the number of active and hospitalised COVID-19 cases in each WWTP catchment area and the concentration of SARS-CoV-2 RNA detected in the wastewater clearly demonstrated the suitability of this wastewater -based epidemiological approach for WWTPs of different sizes and characteristics, despite differences in SARS-CoV-2 variant waves, with some WWTPs showing high predictive potential. This study demonstrated on the data from the Czech Republic that targeted systematic monitoring of wastewater provides sufficiently robust data for surveillance of viral loads in sample populations, and thus contributes to preventing the spread of infection and subsequent introduction of appropriate measures.
ER  -

SOVOVÁ, Kateřina, Petra VAŠÍČKOVÁ, Vojtěch VALÁŠEK, David VÝRAVSKÝ, Věra OČENÁŠKOVÁ, Eva JURANOVÁ, Milena BUŠOVÁ, Milan TUČEK, Vladimír BENCKO and Hana ZVĚŘINOVÁ MLEJNKOVÁ. SARS-CoV-2 wastewater surveillance in the Czech Republic: Spatial and temporal differences in SARS-CoV-2 RNA concentrations and relationship to clinical data and wastewater parameters. \textit{Water Research X}. Elsevier Ltd, 2024, vol.~23, May 2024, p.~1-8. ISSN~2589-9147. Available from: https://dx.doi.org/10.1016/j.wroa.2024.100220.

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