Chloroplast Auxin Efflux Mediated by ABCB28 and ABCB29
Fine-Tunes Salt and Drought Stress Responses in Arabidopsis

J 2024

Chloroplast Auxin Efflux Mediated by ABCB28 and ABCB29 Fine-Tunes Salt and Drought Stress Responses in Arabidopsis

TAMIZHSELVAN, Prashanth, Sharmila MADHAVAN, Christian CONSTAN-AGUILAR, Eman ELREFAAY, Jie LIU et. al.

Basic information

Original name

Chloroplast Auxin Efflux Mediated by ABCB28 and ABCB29 Fine-Tunes Salt and Drought Stress Responses in Arabidopsis

Authors

TAMIZHSELVAN, Prashanth, Sharmila MADHAVAN, Christian CONSTAN-AGUILAR, Eman ELREFAAY, Jie LIU, Ales PENCIK, Ondrej NOVAK, Albert CAIRO, Monika HRTYAN, Markus GEISLER and Vanesa Beatriz TOGNETTI

Edition

PLANTS-BASEL, BASEL, MDPI, 2024, 2223-7747

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10611 Plant sciences, botany

Country of publisher

Switzerland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 4.500 in 2022

DOI

http://dx.doi.org/10.3390/plants13010007

UT WoS

001141318500001

Keywords in English

auxin; hormone transport; ABC transporter; stress; photosynthesis; drought; salinity

Abstract

V originále

Photosynthesis is among the first processes negatively affected by environmental cues and its performance directly determines plant cell fitness and ultimately crop yield. Primarily sites of photosynthesis, chloroplasts are unique sites also for the biosynthesis of precursors of the growth regulator auxin and for sensing environmental stress, but their role in intracellular auxin homeostasis, vital for plant growth and survival in changing environments, remains poorly understood. Here, we identified two ATP-binding cassette (ABC) subfamily B transporters, ABCB28 and ABCB29, which export auxin across the chloroplast envelope to the cytosol in a concerted action in vivo. Moreover, we provide evidence for an auxin biosynthesis pathway in Arabidopsis thaliana chloroplasts. The overexpression of ABCB28 and ABCB29 influenced stomatal regulation and resulted in significantly improved water use efficiency and survival rates during salt and drought stresses. Our results suggest that chloroplast auxin production and transport contribute to stomata regulation for conserving water upon salt stress. ABCB28 and ABCB29 integrate photosynthesis and auxin signals and as such hold great potential to improve the adaptation potential of crops to environmental cues.

Links

GA15-16520S, research and development project

Name: INTERAKCE ROS A AUXINU BĚHEM VÝVOJE ROSTLIN A ADAPTACE VŮČI STRESŮM

Investor: Czech Science Foundation

Citovat

TAMIZHSELVAN, Prashanth, Sharmila MADHAVAN, Christian CONSTAN-AGUILAR, Eman ELREFAAY, Jie LIU, Ales PENCIK, Ondrej NOVAK, Albert CAIRO, Monika HRTYAN, Markus GEISLER and Vanesa Beatriz TOGNETTI. Chloroplast Auxin Efflux Mediated by ABCB28 and ABCB29 Fine-Tunes Salt and Drought Stress Responses in Arabidopsis. PLANTS-BASEL. BASEL: MDPI, 2024, vol. 13, No 1, p. 1-26. ISSN 2223-7747. Available from: https://dx.doi.org/10.3390/plants13010007.

@article{2409537,
   author = {Tamizhselvan, Prashanth and Madhavan, Sharmila and ConstanandAguilar, Christian and Elrefaay, Eman and Liu, Jie and Pencik, Ales and Novak, Ondrej and Cairo, Albert and Hrtyan, Monika and Geisler, Markus and Tognetti, Vanesa Beatriz},
   article_location = {BASEL},
   article_number = {1},
   doi = {http://dx.doi.org/10.3390/plants13010007},
   keywords = {auxin; hormone transport; ABC transporter; stress; photosynthesis; drought; salinity},
   language = {eng},
   issn = {2223-7747},
   journal = {PLANTS-BASEL},
   title = {Chloroplast Auxin Efflux Mediated by ABCB28 and ABCB29 Fine-Tunes Salt and Drought Stress Responses in Arabidopsis},
   url = {https://www.mdpi.com/2223-7747/13/1/7},
   volume = {13},
   year = {2024}
}

TY  - JOUR
ID  - 2409537
AU  - Tamizhselvan, Prashanth - Madhavan, Sharmila - Constan-Aguilar, Christian - Elrefaay, Eman - Liu, Jie - Pencik, Ales - Novak, Ondrej - Cairo, Albert - Hrtyan, Monika - Geisler, Markus - Tognetti, Vanesa Beatriz
PY  - 2024
TI  - Chloroplast Auxin Efflux Mediated by ABCB28 and ABCB29 Fine-Tunes Salt and Drought Stress Responses in Arabidopsis
JF  - PLANTS-BASEL
VL  - 13
IS  - 1
SP  - 1-26
EP  - 1-26
PB  - MDPI
SN  - 22237747
KW  - auxin
KW  - hormone transport
KW  - ABC transporter
KW  - stress
KW  - photosynthesis
KW  - drought
KW  - salinity
UR  - https://www.mdpi.com/2223-7747/13/1/7
N2  - Photosynthesis is among the first processes negatively affected by environmental cues and its performance directly determines plant cell fitness and ultimately crop yield. Primarily sites of photosynthesis, chloroplasts are unique sites also for the biosynthesis of precursors of the growth regulator auxin and for sensing environmental stress, but their role in intracellular auxin homeostasis, vital for plant growth and survival in changing environments, remains poorly understood. Here, we identified two ATP-binding cassette (ABC) subfamily B transporters, ABCB28 and ABCB29, which export auxin across the chloroplast envelope to the cytosol in a concerted action in vivo. Moreover, we provide evidence for an auxin biosynthesis pathway in Arabidopsis thaliana chloroplasts. The overexpression of ABCB28 and ABCB29 influenced stomatal regulation and resulted in significantly improved water use efficiency and survival rates during salt and drought stresses. Our results suggest that chloroplast auxin production and transport contribute to stomata regulation for conserving water upon salt stress. ABCB28 and ABCB29 integrate photosynthesis and auxin signals and as such hold great potential to improve the adaptation potential of crops to environmental cues.
ER  -

TAMIZHSELVAN, Prashanth, Sharmila MADHAVAN, Christian CONSTAN-AGUILAR, Eman ELREFAAY, Jie LIU, Ales PENCIK, Ondrej NOVAK, Albert CAIRO, Monika HRTYAN, Markus GEISLER and Vanesa Beatriz TOGNETTI. Chloroplast Auxin Efflux Mediated by ABCB28 and ABCB29 Fine-Tunes Salt and Drought Stress Responses in Arabidopsis. \textit{PLANTS-BASEL}. BASEL: MDPI, 2024, vol.~13, No~1, p.~1-26. ISSN~2223-7747. Available from: https://dx.doi.org/10.3390/plants13010007.

Detailed Information on Publication Record