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@article{2409586,
author = {Kanova, Evelina and Tkacova, Zuzana and Bhide, Katarina and Kulkarni, Amod and JimenezandMunguia, Irene and Mertinkova, Patricia and Drazovska, Monika and Tyagi, Punit and Bhide, Mangesh},
article_location = {London},
article_number = {Dec},
doi = {http://dx.doi.org/10.1038/s41598-019-55409-y},
keywords = {MEMBRANE VESICLE VACCINE; IV PILI; DEATH RECEPTORS; INFECTION; INVASION; ACTIVATION; CROSS; RECRUITMENT; GONORRHOEA; EXPRESSION},
language = {eng},
issn = {2045-2322},
journal = {Nature Scientific Reports},
title = {Transcriptome analysis of human brain microvascular endothelial cells response to <i>Neisseria meningitidis</i> and its antigen MafA using RNA-seq},
url = {https://www.nature.com/articles/s41598-019-55409-y},
volume = {9},
year = {2019}
}
TY - JOUR
ID - 2409586
AU - Kanova, Evelina - Tkacova, Zuzana - Bhide, Katarina - Kulkarni, Amod - Jimenez-Munguia, Irene - Mertinkova, Patricia - Drazovska, Monika - Tyagi, Punit - Bhide, Mangesh
PY - 2019
TI - Transcriptome analysis of human brain microvascular endothelial cells response to <i>Neisseria meningitidis</i> and its antigen MafA using RNA-seq
JF - Nature Scientific Reports
VL - 9
IS - Dec
SP - 1-16
EP - 1-16
PB - NATURE RESEARCH
SN - 20452322
KW - MEMBRANE VESICLE VACCINE
KW - IV PILI
KW - DEATH RECEPTORS
KW - INFECTION
KW - INVASION
KW - ACTIVATION
KW - CROSS
KW - RECRUITMENT
KW - GONORRHOEA
KW - EXPRESSION
UR - https://www.nature.com/articles/s41598-019-55409-y
N2 - Interaction of Neisseria meningitidis (NM) with human brain microvascular endothelial cells (hBMECs) initiates of multiple cellular processes, which allow bacterial translocation across the blood-brain barrier (BBB). NM is equipped with several antigens, which interacts with the host cell receptors. Recently we have shown that adhesin MafA (UniProtKB-X5EG71), relatively less studied protein, is one of those surface exposed antigens that adhere to hBMECs. The present study was designed to comprehensively map the undergoing biological processes in hBMECs challenged with NM or MafA using RNA sequencing. 708 and 726 differentially expressed genes (DEGs) were identified in hBMECs exposed to NM and MafA, respectively. Gene ontology analysis of the DEGs revealed that several biological processes, which may alter the permeability of BBB, were activated. Comparative analysis of DEGs revealed that MafA, alike NM, might provoke TLR-dependent pathway and augment cytokine response. Moreover, both MafA and NM were able to induce genes involved in cell surface modifications, endocytosis, extracellular matrix remodulation and anoikis/apoptosis. In conclusion, this study for the first time describes effect of NM on the global gene expression in hBMECs using high-throughput RNA-seq. It also presents ability of MafA to induce gene expression, which might aid NM in breaching the BBB.
ER -
KANOVA, Evelina, Zuzana TKACOVA, Katarina BHIDE, Amod KULKARNI, Irene JIMENEZ-MUNGUIA, Patricia MERTINKOVA, Monika DRAZOVSKA, Punit TYAGI and Mangesh BHIDE. Transcriptome analysis of human brain microvascular endothelial cells response to \&{}lt;i\&{}gt;Neisseria meningitidis\&{}lt;/i\&{}gt; and its antigen MafA using RNA-seq. \textit{Nature Scientific Reports}. London: NATURE RESEARCH, 2019, vol.~9, Dec, p.~1-16. ISSN~2045-2322. Available from: https://dx.doi.org/10.1038/s41598-019-55409-y.
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