J 2019

Transcriptomic Analysis Reveals the Roles of Detoxification Systems in Response to Mercury in <i>Chromera velia</i>

SHARAF, Abdoallah, De Michele ROBERTO, Ayush SHARMA, Safieh FAKHARI, Miroslav OBORNIK et. al.

Basic information

Original name

Transcriptomic Analysis Reveals the Roles of Detoxification Systems in Response to Mercury in <i>Chromera velia</i>

Authors

SHARAF, Abdoallah, De Michele ROBERTO, Ayush SHARMA, Safieh FAKHARI and Miroslav OBORNIK

Edition

Biomolecules, Basel, Switzerland, MDPI AG, 2019, 2218-273X

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10608 Biochemistry and molecular biology

Country of publisher

Switzerland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

Impact factor

Impact factor: 4.082

DOI

UT WoS

000502267900008

Keywords in English

chromerids; transcriptome; heavy metal; antioxidant enzymes; xenobiotics; phylogenies; reactive oxygen species; reactive nitrogen species

Tags

Tags

International impact, Reviewed
Změněno: 10/6/2024 16:57, Mgr. Eva Dubská

Abstract

V originále

Heavy metal pollution is an increasing global concern. Among heavy metals, mercury (Hg) is especially dangerous because of its massive release into the environment and high toxicity, especially for aquatic organisms. The molecular response mechanisms of algae to Hg exposure are mostly unknown. Here, we combine physiological, biochemical, and transcriptomic analysis to provide, for the first time, a comprehensive view on the pathways activated in Chromera velia in response to toxic levels of Hg. Production of hydrogen peroxide and superoxide anion, two reactive oxygen species (ROS), showed opposite patterns in response to Hg2+ while reactive nitrogen species (RNS) levels did not change. A deep RNA sequencing analysis generated a total of 307,738,790 high-quality reads assembled in 122,874 transcripts, representing 89,853 unigenes successfully annotated in databases. Detailed analysis of the differently expressed genes corroborates the biochemical results observed in ROS production and suggests novel putative molecular mechanisms in the algal response to Hg2+. Moreover, we indicated that important transcription factor (TF) families associated with stress responses differentially expressed in C. velia cultures under Hg stress. Our study presents the first in-depth transcriptomic analysis of C. velia, focusing on the expression of genes involved in different detoxification defense systems in response to heavy metal stress.