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@article{2409618,
author = {Sharaf, Abdoallah and Roberto, De Michele and Sharma, Ayush and Fakhari, Safieh and Obornik, Miroslav},
article_location = {Basel, Switzerland},
article_number = {11},
doi = {http://dx.doi.org/10.3390/biom9110647},
keywords = {chromerids; transcriptome; heavy metal; antioxidant enzymes; xenobiotics; phylogenies; reactive oxygen species; reactive nitrogen species},
language = {eng},
issn = {2218-273X},
journal = {Biomolecules},
title = {Transcriptomic Analysis Reveals the Roles of Detoxification Systems in Response to Mercury in <i>Chromera velia</i>},
url = {https://www.mdpi.com/2218-273X/9/11/647},
volume = {9},
year = {2019}
}
TY - JOUR
ID - 2409618
AU - Sharaf, Abdoallah - Roberto, De Michele - Sharma, Ayush - Fakhari, Safieh - Obornik, Miroslav
PY - 2019
TI - Transcriptomic Analysis Reveals the Roles of Detoxification Systems in Response to Mercury in <i>Chromera velia</i>
JF - Biomolecules
VL - 9
IS - 11
SP - 1-25
EP - 1-25
PB - MDPI AG
SN - 2218273X
KW - chromerids
KW - transcriptome
KW - heavy metal
KW - antioxidant enzymes
KW - xenobiotics
KW - phylogenies
KW - reactive oxygen species
KW - reactive nitrogen species
UR - https://www.mdpi.com/2218-273X/9/11/647
N2 - Heavy metal pollution is an increasing global concern. Among heavy metals, mercury (Hg) is especially dangerous because of its massive release into the environment and high toxicity, especially for aquatic organisms. The molecular response mechanisms of algae to Hg exposure are mostly unknown. Here, we combine physiological, biochemical, and transcriptomic analysis to provide, for the first time, a comprehensive view on the pathways activated in Chromera velia in response to toxic levels of Hg. Production of hydrogen peroxide and superoxide anion, two reactive oxygen species (ROS), showed opposite patterns in response to Hg2+ while reactive nitrogen species (RNS) levels did not change. A deep RNA sequencing analysis generated a total of 307,738,790 high-quality reads assembled in 122,874 transcripts, representing 89,853 unigenes successfully annotated in databases. Detailed analysis of the differently expressed genes corroborates the biochemical results observed in ROS production and suggests novel putative molecular mechanisms in the algal response to Hg2+. Moreover, we indicated that important transcription factor (TF) families associated with stress responses differentially expressed in C. velia cultures under Hg stress. Our study presents the first in-depth transcriptomic analysis of C. velia, focusing on the expression of genes involved in different detoxification defense systems in response to heavy metal stress.
ER -
SHARAF, Abdoallah, De Michele ROBERTO, Ayush SHARMA, Safieh FAKHARI and Miroslav OBORNIK. Transcriptomic Analysis Reveals the Roles of Detoxification Systems in Response to Mercury in \&{}lt;i\&{}gt;Chromera velia\&{}lt;/i\&{}gt;. \textit{Biomolecules}. Basel, Switzerland: MDPI AG, 2019, vol.~9, No~11, p.~1-25. ISSN~2218-273X. Available from: https://dx.doi.org/10.3390/biom9110647.
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