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@article{2409817,
author = {Nemeckova, Alzbeta and Kolackova, Veronika and Vrana, Jan and Dolezel, Jaroslav and Hribova, Eva},
article_location = {Oxford},
article_number = {20},
doi = {http://dx.doi.org/10.1093/jxb/eraa370},
keywords = {DNA replication; EdU labeling; flow cytometry; Poaceae; Rabl configuration; S phase; three-dimensional fluorescence in situ hybridization (3D-FISH)},
language = {eng},
issn = {0022-0957},
journal = {Journal of Experimental Botany},
title = {DNA replication and chromosome positioning throughout the interphase in three-dimensional space of plant nuclei},
url = {https://academic.oup.com/jxb/article/71/20/6262/5893557?login=true},
volume = {71},
year = {2020}
}
TY - JOUR
ID - 2409817
AU - Nemeckova, Alzbeta - Kolackova, Veronika - Vrana, Jan - Dolezel, Jaroslav - Hribova, Eva
PY - 2020
TI - DNA replication and chromosome positioning throughout the interphase in three-dimensional space of plant nuclei
JF - Journal of Experimental Botany
VL - 71
IS - 20
SP - 6262-6272
EP - 6262-6272
PB - OXFORD UNIV PRESS
SN - 00220957
KW - DNA replication
KW - EdU labeling
KW - flow cytometry
KW - Poaceae
KW - Rabl configuration
KW - S phase
KW - three-dimensional fluorescence in situ hybridization (3D-FISH)
UR - https://academic.oup.com/jxb/article/71/20/6262/5893557?login=true
N2 - Despite much recent progress, our understanding of the principles of plant genome organization and its dynamics in three-dimensional space of interphase nuclei remains surprisingly limited. Notably, it is not clear how these processes could be affected by the size of a plant's nuclear genome. In this study, DNA replication timing and interphase chromosome positioning were analyzed in seven Poaceae species that differ in their genome size. To provide a comprehensive picture, a suite of advanced, complementary methods was used: labeling of newly replicated DNA by ethynyl-2'-deoxyuridine, isolation of nuclei at particular cell cycle phases by flow cytometric sorting, three-dimensional immunofluorescence in situ hybridization, and confocal microscopy. Our results revealed conserved dynamics of DNA replication in all species, and a similar replication timing order for telomeres and centromeres, as well as for euchromatin and heterochromatin regions, irrespective of genome size. Moreover, stable chromosome positioning was observed while transitioning through different stages of interphase. These findings expand upon earlier studies in suggesting that a more complex interplay exists between genome size, organization of repetitive DNA sequences along chromosomes, and higher order chromatin structure and its maintenance in interphase, albeit controlled by currently unknown factors.
ER -
NEMECKOVA, Alzbeta, Veronika KOLACKOVA, Jan VRANA, Jaroslav DOLEZEL and Eva HRIBOVA. DNA replication and chromosome positioning throughout the interphase in three-dimensional space of plant nuclei. \textit{Journal of Experimental Botany}. Oxford: OXFORD UNIV PRESS, 2020, vol.~71, No~20, p.~6262-6272. ISSN~0022-0957. Available from: https://dx.doi.org/10.1093/jxb/eraa370.
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