J 2020

DNA replication and chromosome positioning throughout the interphase in three-dimensional space of plant nuclei

NEMECKOVA, Alzbeta, Veronika KOLACKOVA, Jan VRANA, Jaroslav DOLEZEL, Eva HRIBOVA et. al.

Basic information

Original name

DNA replication and chromosome positioning throughout the interphase in three-dimensional space of plant nuclei

Authors

NEMECKOVA, Alzbeta, Veronika KOLACKOVA, Jan VRANA, Jaroslav DOLEZEL and Eva HRIBOVA

Edition

Journal of Experimental Botany, Oxford, OXFORD UNIV PRESS, 2020, 0022-0957

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10611 Plant sciences, botany

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

Impact factor

Impact factor: 6.992

DOI

UT WoS

000605983900008

Keywords in English

DNA replication; EdU labeling; flow cytometry; Poaceae; Rabl configuration; S phase; three-dimensional fluorescence in situ hybridization (3D-FISH)

Tags

Tags

International impact, Reviewed
Změněno: 9/10/2024 13:06, Mgr. Adéla Pešková

Abstract

V originále

Despite much recent progress, our understanding of the principles of plant genome organization and its dynamics in three-dimensional space of interphase nuclei remains surprisingly limited. Notably, it is not clear how these processes could be affected by the size of a plant's nuclear genome. In this study, DNA replication timing and interphase chromosome positioning were analyzed in seven Poaceae species that differ in their genome size. To provide a comprehensive picture, a suite of advanced, complementary methods was used: labeling of newly replicated DNA by ethynyl-2'-deoxyuridine, isolation of nuclei at particular cell cycle phases by flow cytometric sorting, three-dimensional immunofluorescence in situ hybridization, and confocal microscopy. Our results revealed conserved dynamics of DNA replication in all species, and a similar replication timing order for telomeres and centromeres, as well as for euchromatin and heterochromatin regions, irrespective of genome size. Moreover, stable chromosome positioning was observed while transitioning through different stages of interphase. These findings expand upon earlier studies in suggesting that a more complex interplay exists between genome size, organization of repetitive DNA sequences along chromosomes, and higher order chromatin structure and its maintenance in interphase, albeit controlled by currently unknown factors.