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@article{2409862,
author = {Gergelits, Vaclav and Parvanov, Emil and Simecek, Petr and Forejt, Jiri},
article_location = {BETHESDA (USA)},
article_number = {1},
doi = {http://dx.doi.org/10.1093/genetics/iyaa013},
keywords = {homologous recombination; gene conversion; PRDM9 motif erosion; hybrid sterility; noncrossover-associated GC bias},
language = {eng},
issn = {0016-6731},
journal = {Genetics},
title = {Chromosome-wide characterization of meiotic noncrossovers (gene conversions) in mouse hybrids},
url = {https://academic.oup.com/genetics/article/217/1/iyaa013/5998651?login=true},
volume = {217},
year = {2021}
}
TY - JOUR
ID - 2409862
AU - Gergelits, Vaclav - Parvanov, Emil - Simecek, Petr - Forejt, Jiri
PY - 2021
TI - Chromosome-wide characterization of meiotic noncrossovers (gene conversions) in mouse hybrids
JF - Genetics
VL - 217
IS - 1
PB - GENETICS SOCIETY AMERICA
SN - 00166731
KW - homologous recombination
KW - gene conversion
KW - PRDM9 motif erosion
KW - hybrid sterility
KW - noncrossover-associated GC bias
UR - https://academic.oup.com/genetics/article/217/1/iyaa013/5998651?login=true
N2 - During meiosis, the recombination-initiating DNA double-strand breaks (DSBs) are repaired by crossovers or noncrossovers (gene conversions). While crossovers are easily detectable, noncrossover identification is hampered by the small size of their converted tracts and the necessity of sequence polymorphism. We report identification and characterization of a mouse chromosome-wide set of noncrossovers by next-generation sequencing of 10 mouse intersubspecific chromosome substitution strains. Based on 94 identified noncrossovers, we determined the mean length of a conversion tract to be 32 bp. The spatial chromosome-wide distribution of noncrossovers and crossovers significantly differed, although both sets overlapped the known hotspots of PRDM9-directed histone methylation and DNA DSBs, thus supporting their origin in the standard DSB repair pathway. A significant deficit of noncrossovers descending from asymmetric DSBs proved their proposed adverse effect on meiotic recombination and pointed to sister chromatids as an alternative template for their repair. The finding has implications for the molecular mechanism of hybrid sterility in mice from crosses between closely related Mus musculus musculus and Mus musculus domesticus subspecies.
ER -
GERGELITS, Vaclav, Emil PARVANOV, Petr SIMECEK and Jiri FOREJT. Chromosome-wide characterization of meiotic noncrossovers (gene conversions) in mouse hybrids. \textit{Genetics}. BETHESDA (USA): GENETICS SOCIETY AMERICA, 2021, vol.~217, No~1, 14 pp. ISSN~0016-6731. Available from: https://dx.doi.org/10.1093/genetics/iyaa013.
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