Chromosome-wide characterization of meiotic noncrossovers (gene
conversions) in mouse hybrids

J 2021

Chromosome-wide characterization of meiotic noncrossovers (gene conversions) in mouse hybrids

GERGELITS, Vaclav, Emil PARVANOV, Petr SIMECEK and Jiri FOREJT

Basic information

Original name

Chromosome-wide characterization of meiotic noncrossovers (gene conversions) in mouse hybrids

Authors

GERGELITS, Vaclav, Emil PARVANOV, Petr SIMECEK and Jiri FOREJT

Edition

Genetics, BETHESDA (USA), GENETICS SOCIETY AMERICA, 2021, 0016-6731

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10603 Genetics and heredity

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 4.402

DOI

http://dx.doi.org/10.1093/genetics/iyaa013

UT WoS

000637217400010

Keywords in English

homologous recombination; gene conversion; PRDM9 motif erosion; hybrid sterility; noncrossover-associated GC bias

Abstract

V originále

During meiosis, the recombination-initiating DNA double-strand breaks (DSBs) are repaired by crossovers or noncrossovers (gene conversions). While crossovers are easily detectable, noncrossover identification is hampered by the small size of their converted tracts and the necessity of sequence polymorphism. We report identification and characterization of a mouse chromosome-wide set of noncrossovers by next-generation sequencing of 10 mouse intersubspecific chromosome substitution strains. Based on 94 identified noncrossovers, we determined the mean length of a conversion tract to be 32 bp. The spatial chromosome-wide distribution of noncrossovers and crossovers significantly differed, although both sets overlapped the known hotspots of PRDM9-directed histone methylation and DNA DSBs, thus supporting their origin in the standard DSB repair pathway. A significant deficit of noncrossovers descending from asymmetric DSBs proved their proposed adverse effect on meiotic recombination and pointed to sister chromatids as an alternative template for their repair. The finding has implications for the molecular mechanism of hybrid sterility in mice from crosses between closely related Mus musculus musculus and Mus musculus domesticus subspecies.

Links

90132, large research infrastructures

Name: NCMG II

Citovat

GERGELITS, Vaclav, Emil PARVANOV, Petr SIMECEK and Jiri FOREJT. Chromosome-wide characterization of meiotic noncrossovers (gene conversions) in mouse hybrids. Genetics. BETHESDA (USA): GENETICS SOCIETY AMERICA, 2021, vol. 217, No 1, 14 pp. ISSN 0016-6731. Available from: https://dx.doi.org/10.1093/genetics/iyaa013.

@article{2409862,
   author = {Gergelits, Vaclav and Parvanov, Emil and Simecek, Petr and Forejt, Jiri},
   article_location = {BETHESDA (USA)},
   article_number = {1},
   doi = {http://dx.doi.org/10.1093/genetics/iyaa013},
   keywords = {homologous recombination; gene conversion; PRDM9 motif erosion; hybrid sterility; noncrossover-associated GC bias},
   language = {eng},
   issn = {0016-6731},
   journal = {Genetics},
   title = {Chromosome-wide characterization of meiotic noncrossovers (gene conversions) in mouse hybrids},
   url = {https://academic.oup.com/genetics/article/217/1/iyaa013/5998651?login=true},
   volume = {217},
   year = {2021}
}

TY  - JOUR
ID  - 2409862
AU  - Gergelits, Vaclav - Parvanov, Emil - Simecek, Petr - Forejt, Jiri
PY  - 2021
TI  - Chromosome-wide characterization of meiotic noncrossovers (gene conversions) in mouse hybrids
JF  - Genetics
VL  - 217
IS  - 1
PB  - GENETICS SOCIETY AMERICA
SN  - 00166731
KW  - homologous recombination
KW  - gene conversion
KW  - PRDM9 motif erosion
KW  - hybrid sterility
KW  - noncrossover-associated GC bias
UR  - https://academic.oup.com/genetics/article/217/1/iyaa013/5998651?login=true
N2  - During meiosis, the recombination-initiating DNA double-strand breaks (DSBs) are repaired by crossovers or noncrossovers (gene conversions). While crossovers are easily detectable, noncrossover identification is hampered by the small size of their converted tracts and the necessity of sequence polymorphism. We report identification and characterization of a mouse chromosome-wide set of noncrossovers by next-generation sequencing of 10 mouse intersubspecific chromosome substitution strains. Based on 94 identified noncrossovers, we determined the mean length of a conversion tract to be 32 bp. The spatial chromosome-wide distribution of noncrossovers and crossovers significantly differed, although both sets overlapped the known hotspots of PRDM9-directed histone methylation and DNA DSBs, thus supporting their origin in the standard DSB repair pathway. A significant deficit of noncrossovers descending from asymmetric DSBs proved their proposed adverse effect on meiotic recombination and pointed to sister chromatids as an alternative template for their repair. The finding has implications for the molecular mechanism of hybrid sterility in mice from crosses between closely related Mus musculus musculus and Mus musculus domesticus subspecies.
ER  -

GERGELITS, Vaclav, Emil PARVANOV, Petr SIMECEK and Jiri FOREJT. Chromosome-wide characterization of meiotic noncrossovers (gene conversions) in mouse hybrids. \textit{Genetics}. BETHESDA (USA): GENETICS SOCIETY AMERICA, 2021, vol.~217, No~1, 14 pp. ISSN~0016-6731. Available from: https://dx.doi.org/10.1093/genetics/iyaa013.

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