Lipid Polymorphism of the Subchloroplast-Granum and Stroma
Thylakoid Membrane-Particles. II. Structure and Functions

J 2021

Lipid Polymorphism of the Subchloroplast-Granum and Stroma Thylakoid Membrane-Particles. II. Structure and Functions

DLOUHY, Ondrej, Vaclav KARLICKY, Rameez ARSHAD, Otto ZSIROS, Ildiko DOMONKOS et. al.

Basic information

Original name

Lipid Polymorphism of the Subchloroplast-Granum and Stroma Thylakoid Membrane-Particles. II. Structure and Functions

Authors

DLOUHY, Ondrej, Vaclav KARLICKY, Rameez ARSHAD, Otto ZSIROS, Ildiko DOMONKOS, Irena KURASOVA, Andras F WACHA, Tomas MOROSINOTTO, Attila BOTA, Roman KOURIL, Vladimir SPUNDA and Gyozo GARAB

Edition

Advances in Fuel Cells, BASEL, Elsevier, 2021, 1752-301X

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10601 Cell biology

Country of publisher

Switzerland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

DOI

http://dx.doi.org/10.3390/cells10092363

UT WoS

000699266100001

Keywords in English

bilayer; chlorophyll fluorescence; cryo-electron-tomography; electron microscopy; membrane energization; membrane networks; non-bilayer lipid phases; SAXS; violaxanthin de-epoxidase

Abstract

V originále

In Part I, by using P-31-NMR spectroscopy, we have shown that isolated granum and stroma thylakoid membranes (TMs), in addition to the bilayer, display two isotropic phases and an inverted hexagonal (H-II) phase; saturation transfer experiments and selective effects of lipase and thermal treatments have shown that these phases arise from distinct, yet interconnectable structural entities. To obtain information on the functional roles and origin of the different lipid phases, here we performed spectroscopic measurements and inspected the ultrastructure of these TM fragments. Circular dichroism, 77 K fluorescence emission spectroscopy, and variable chlorophyll-a fluorescence measurements revealed only minor lipase- or thermally induced changes in the photosynthetic machinery. Electrochromic absorbance transients showed that the TM fragments were re-sealed, and the vesicles largely retained their impermeabilities after lipase treatments-in line with the low susceptibility of the bilayer against the same treatment, as reflected by our P-31-NMR spectroscopy. Signatures of H-II-phase could not be discerned with small-angle X-ray scattering-but traces of H-II structures, without long-range order, were found by freeze-fracture electron microscopy (FF-EM) and cryo-electron tomography (CET). EM and CET images also revealed the presence of small vesicles and fusion of membrane particles, which might account for one of the isotropic phases. Interaction of VDE (violaxanthin de-epoxidase, detected by Western blot technique in both membrane fragments) with TM lipids might account for the other isotropic phase. In general, non-bilayer lipids are proposed to play role in the self-assembly of the highly organized yet dynamic TM network in chloroplasts.

Links

90043, large research infrastructures

Name: CIISB

Citovat

DLOUHY, Ondrej, Vaclav KARLICKY, Rameez ARSHAD, Otto ZSIROS, Ildiko DOMONKOS, Irena KURASOVA, Andras F WACHA, Tomas MOROSINOTTO, Attila BOTA, Roman KOURIL, Vladimir SPUNDA and Gyozo GARAB. Lipid Polymorphism of the Subchloroplast-Granum and Stroma Thylakoid Membrane-Particles. II. Structure and Functions. Advances in Fuel Cells. BASEL: Elsevier, 2021, vol. 10, No 9, p. 1-19. ISSN 1752-301X. Available from: https://dx.doi.org/10.3390/cells10092363.

@article{2409863,
   author = {Dlouhy, Ondrej and Karlicky, Vaclav and Arshad, Rameez and Zsiros, Otto and Domonkos, Ildiko and Kurasova, Irena and Wacha, Andras F and Morosinotto, Tomas and Bota, Attila and Kouril, Roman and Spunda, Vladimir and Garab, Gyozo},
   article_location = {BASEL},
   article_number = {9},
   doi = {http://dx.doi.org/10.3390/cells10092363},
   keywords = {bilayer; chlorophyll fluorescence; cryo-electron-tomography; electron microscopy; membrane energization; membrane networks; non-bilayer lipid phases; SAXS; violaxanthin de-epoxidase},
   language = {eng},
   issn = {1752-301X},
   journal = {Advances in Fuel Cells},
   title = {Lipid Polymorphism of the Subchloroplast-Granum and Stroma Thylakoid Membrane-Particles. II. Structure and Functions},
   url = {https://www.mdpi.com/2073-4409/10/9/2363},
   volume = {10},
   year = {2021}
}

TY  - JOUR
ID  - 2409863
AU  - Dlouhy, Ondrej - Karlicky, Vaclav - Arshad, Rameez - Zsiros, Otto - Domonkos, Ildiko - Kurasova, Irena - Wacha, Andras F - Morosinotto, Tomas - Bota, Attila - Kouril, Roman - Spunda, Vladimir - Garab, Gyozo
PY  - 2021
TI  - Lipid Polymorphism of the Subchloroplast-Granum and Stroma Thylakoid Membrane-Particles. II. Structure and Functions
JF  - Advances in Fuel Cells
VL  - 10
IS  - 9
SP  - 1-19
EP  - 1-19
PB  - Elsevier
SN  - 1752301X
KW  - bilayer
KW  - chlorophyll fluorescence
KW  - cryo-electron-tomography
KW  - electron microscopy
KW  - membrane energization
KW  - membrane networks
KW  - non-bilayer lipid phases
KW  - SAXS
KW  - violaxanthin de-epoxidase
UR  - https://www.mdpi.com/2073-4409/10/9/2363
N2  - In Part I, by using P-31-NMR spectroscopy, we have shown that isolated granum and stroma thylakoid membranes (TMs), in addition to the bilayer, display two isotropic phases and an inverted hexagonal (H-II) phase; saturation transfer experiments and selective effects of lipase and thermal treatments have shown that these phases arise from distinct, yet interconnectable structural entities. To obtain information on the functional roles and origin of the different lipid phases, here we performed spectroscopic measurements and inspected the ultrastructure of these TM fragments. Circular dichroism, 77 K fluorescence emission spectroscopy, and variable chlorophyll-a fluorescence measurements revealed only minor lipase- or thermally induced changes in the photosynthetic machinery. Electrochromic absorbance transients showed that the TM fragments were re-sealed, and the vesicles largely retained their impermeabilities after lipase treatments-in line with the low susceptibility of the bilayer against the same treatment, as reflected by our P-31-NMR spectroscopy. Signatures of H-II-phase could not be discerned with small-angle X-ray scattering-but traces of H-II structures, without long-range order, were found by freeze-fracture electron microscopy (FF-EM) and cryo-electron tomography (CET). EM and CET images also revealed the presence of small vesicles and fusion of membrane particles, which might account for one of the isotropic phases. Interaction of VDE (violaxanthin de-epoxidase, detected by Western blot technique in both membrane fragments) with TM lipids might account for the other isotropic phase. In general, non-bilayer lipids are proposed to play role in the self-assembly of the highly organized yet dynamic TM network in chloroplasts.
ER  -

DLOUHY, Ondrej, Vaclav KARLICKY, Rameez ARSHAD, Otto ZSIROS, Ildiko DOMONKOS, Irena KURASOVA, Andras F WACHA, Tomas MOROSINOTTO, Attila BOTA, Roman KOURIL, Vladimir SPUNDA and Gyozo GARAB. Lipid Polymorphism of the Subchloroplast-Granum and Stroma Thylakoid Membrane-Particles. II. Structure and Functions. \textit{Advances in Fuel Cells}. BASEL: Elsevier, 2021, vol.~10, No~9, p.~1-19. ISSN~1752-301X. Available from: https://dx.doi.org/10.3390/cells10092363.

Detailed Information on Publication Record