J 2024

NSE5 subunit interacts with distant regions of the SMC arms in the <i>Physcomitrium patens</i> SMC5/6 complex

VACULÍKOVÁ, Jitka, Marcela HOLA, Barbora KRÁLOVÁ, Edit LELKES, Barbora ŠTEFANOVIE et. al.

Basic information

Original name

NSE5 subunit interacts with distant regions of the SMC arms in the <i>Physcomitrium patens</i> SMC5/6 complex

Authors

VACULÍKOVÁ, Jitka (203 Czech Republic, belonging to the institution), Marcela HOLA (203 Czech Republic), Barbora KRÁLOVÁ (203 Czech Republic, belonging to the institution), Edit LELKES (703 Slovakia, belonging to the institution), Barbora ŠTEFANOVIE (703 Slovakia, belonging to the institution), Radka VAGNEROVA (203 Czech Republic), Karel J. ANGELIS (203 Czech Republic) and Jan PALEČEK (203 Czech Republic, guarantor, belonging to the institution)

Edition

Plant Journal, Hoboken (USA), Wiley-Blackwell, 2024, 0960-7412

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10608 Biochemistry and molecular biology

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

Impact factor

Impact factor: 7.200 in 2022

Organization unit

Faculty of Science

DOI

UT WoS

001243487100001

Keywords in English

Physcomitrium patens SMC5/6 complex; NSE5/SNI1/SLF1/SIMC1; NSE6/ASAP1/SLF2/KRE29; DNA damage repair; rDNA stability; moss caulonemata development

Tags

International impact, Reviewed
Změněno: 12/8/2024 16:26, doc. Mgr. Jan Paleček, Dr. rer. nat.

Abstract

V originále

Structural maintenance of chromosome (SMC) complexes play roles in cohesion, condensation, replication, transcription, and DNA repair. Their cores are composed of SMC proteins with a unique structure consisting of an ATPase head, long arm, and hinge. SMC complexes form long rod-like structures, which can change to ring-like and elbow-bent conformations upon binding ATP, DNA, and other regulatory factors. These SMC dynamic conformational changes are involved in their loading, translocation, and DNA loop extrusion. Here, we examined the binding and role of the PpNSE5 regulatory factor of Physcomitrium patens PpSMC5/6 complex. We found that the PpNSE5 C-terminal half (aa230-505) is required for binding to its PpNSE6 partner, while the N-terminal half (aa1-230) binds PpSMC subunits. Specifically, the first 71 amino acids of PpNSE5 were required for binding to PpSMC6. Interestingly, the PpNSE5 binding required the PpSMC6 head-proximal joint region and PpSMC5 hinge-proximal arm, suggesting a long distance between binding sites on PpSMC5 and PpSMC6 arms. Therefore, we hypothesize that PpNSE5 either links two antiparallel SMC5/6 complexes or binds one SMC5/6 in elbow-bent conformation, the later model being consistent with the role of NSE5/NSE6 dimer as SMC5/6 loading factor to DNA lesions. In addition, we generated the P. patens Ppnse5KO1 mutant line with an N-terminally truncated version of PpNSE5, which exhibited DNA repair defects while keeping a normal number of rDNA repeats. As the first 71 amino acids of PpNSE5 are required for PpSMC6 binding, our results suggest the role of PpNSE5-PpSMC6 interaction in SMC5/6 loading to DNA lesions.

Links

GA20-05095S, research and development project
Name: Úloha komplexu SMC5/6 a jeho interakčních partnerů v opravě poškozené DNA (Acronym: SMC5/6)
Investor: Czech Science Foundation
MUNI/R/1142/2021, interní kód MU
Name: Visualization of the 3D structure of chromatin
Investor: Masaryk University, CAREER RESTART

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