J 2024

What is the impact of plastic deformation on cytocompatibility of biodegradable Zn–Mg alloys?

WOJTAS, Daniel, Klaudia TREMBECKA-WÓJCIGA, Magdalena GIELECIAK, Agnieszka BIGOS, Kamil BRUDECKI et. al.

Basic information

Original name

What is the impact of plastic deformation on cytocompatibility of biodegradable Zn–Mg alloys?

Authors

WOJTAS, Daniel (616 Poland, belonging to the institution), Klaudia TREMBECKA-WÓJCIGA, Magdalena GIELECIAK, Agnieszka BIGOS, Kamil BRUDECKI, Sylwia PRZYBYSZ-GLOC, Romana SCHIRHAGL, Aldona MZYK and Anna JARZĘBSKA

Edition

MATERIALS ADVANCES, CAMBRIDGE, ROYAL SOC CHEMISTRY, 2024, 2633-5409

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

20601 Medical engineering

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

Impact factor

Impact factor: 5.000 in 2022

Organization unit

Faculty of Medicine

UT WoS

001250669600001

Keywords in English

biodegradable Zn-Mg alloys; plastic deformation; cytocompatibility

Tags

Tags

International impact, Reviewed
Změněno: 5/11/2024 14:08, Mgr. Tereza Miškechová

Abstract

V originále

Research on biodegradable zinc requires thorough in vitro cytotoxicity screening as the developed materials are being proposed for various medical implants, including stents. This study investigates the endothelial cell response to a novel Zn–0.8Mg alloy produced via hydrostatic extrusion (HSE), aiming to showcase the impact of plastic deformation on the cytocompatibility of biodegradable zinc-based materials. In doing so, the MTT test for cell viability studies as well as confocal laser scanning microscopy (CLSM) imaging for cell morphology analysis were used. In addition, a cutting-edge diamond-based quantum sensing technique, i.e., T1 relaxometry, was employed to reveal the nanoscale impact of ions on cells. It was demonstrated that the HSE-produced materials exhibited a 10-fold decrease in grain size, microstructural homogenization, and consequently more uniform degradation compared to hot extruded, coarse-grained materials. Despite these differences, the MTT and CLSM data did not show any drastic discrepancies between the endothelial cell response to any of the investigated materials. However, T1 relaxometry measurements indicated that plastic deformation might influence the cytocompatibility of biodegradable zinc-based materials, as evidenced by significant intracellular free radical production in endothelial cells exposed to ions released from the Zn–0.8Mg HSE alloy surface. Overall, no adverse effects of plastic deformation on the cytocompatibility of zinc-based materials were found as free radical generation may play a beneficial role in endothelial cell function, suggesting a complex interaction between material degradation and cellular response.