Atypical applications of transverse diffusion of laminar flow
profiles methodology for in-capillary reactions in capillary
electrophoresis

J 2024

Atypical applications of transverse diffusion of laminar flow profiles methodology for in-capillary reactions in capillary electrophoresis

BRŽEZICKÁ, Taťána, Lenka KOHÚTOVÁ and Zdeněk GLATZ

Basic information

Original name

Atypical applications of transverse diffusion of laminar flow profiles methodology for in-capillary reactions in capillary electrophoresis

Authors

BRŽEZICKÁ, Taťána (203 Czech Republic, belonging to the institution), Lenka KOHÚTOVÁ (203 Czech Republic, belonging to the institution) and Zdeněk GLATZ (203 Czech Republic, guarantor, belonging to the institution)

Edition

JOURNAL OF SEPARATION SCIENCE, GERMANY, WILEY-V C H VERLAG GMBH, 2024, 1615-9306

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10406 Analytical chemistry

Country of publisher

Germany

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 3.100 in 2022

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.1002/jssc.202400157

UT WoS

001268081000001

Keywords in English

Capillary electrophoresis; In-capillary reaction; High-throughput screening technology; Transverse diffusion of laminar flow profiles methodology; Drug

Abstract

V originále

CE is a powerful separation technique offering quick and efficient analyses in various fields of bioanalytical chemistry. It is characterised by many well-known advantages, but one, which is perhaps the most important for this application field, is somewhat overlooked. It is the possibility of performing chemical and biochemical reactions at the nL scale inside the separation capillary. There are two basic formats applicable for this purpose, heterogeneous and homogeneous. In the former, one reactant is immobilised onto a particle or monolithic support or directly on the capillary wall, and the other is injected. In the latter, the reactant mixing inside a capillary is based on electromigration or diffusion. One of the diffusion-based methodologies, termed Transverse Diffusion of Laminar Flow Profiles, is the subject of this review. Since most studies utilising in-capillary reactions in CE focus on enzymes, which are being continuously and exhaustively reviewed, this review covers the atypical applications of this methodology, but still in the bioanalytical field. As can be seen from the demonstrated applications, they are not limited to reactions, but can also be utilised for other biochemical systems such as affinity interaction studies or biochemically important processes such as cell lysis, even in single-cell format.

Links

GA19-08358S, research and development project

Name: Nové přístupy pro studium afinitních interakcí založené na kapilární elektroforéze

Investor: Czech Science Foundation

NU23-08-00229, research and development project

Name: Minimálně invazivní metody pro personalizovanou farmakoterapii epilepsie

Investor: Ministry of Health of the CR, Minimally invasive methods for the personalized pharmacotherapy of epilepsy, Subprogram 1 - standard

Citovat

BRŽEZICKÁ, Taťána, Lenka KOHÚTOVÁ and Zdeněk GLATZ. Atypical applications of transverse diffusion of laminar flow profiles methodology for in-capillary reactions in capillary electrophoresis. JOURNAL OF SEPARATION SCIENCE. GERMANY: WILEY-V C H VERLAG GMBH, 2024, vol. 47, No 13, p. 1-24. ISSN 1615-9306. Available from: https://dx.doi.org/10.1002/jssc.202400157.

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   author = {Bržezická, Taťána and Kohútová, Lenka and Glatz, Zdeněk},
   article_location = {GERMANY},
   article_number = {13},
   doi = {http://dx.doi.org/10.1002/jssc.202400157},
   keywords = {Capillary electrophoresis; In-capillary reaction; High-throughput screening technology; Transverse diffusion of laminar flow profiles methodology; Drug},
   language = {eng},
   issn = {1615-9306},
   journal = {JOURNAL OF SEPARATION SCIENCE},
   title = {Atypical applications of transverse diffusion of laminar flow profiles methodology for in-capillary reactions in capillary electrophoresis},
   url = {https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/epdf/10.1002/jssc.202400157},
   volume = {47},
   year = {2024}
}

TY  - JOUR
ID  - 2415977
AU  - Bržezická, Taťána - Kohútová, Lenka - Glatz, Zdeněk
PY  - 2024
TI  - Atypical applications of transverse diffusion of laminar flow profiles methodology for in-capillary reactions in capillary electrophoresis
JF  - JOURNAL OF SEPARATION SCIENCE
VL  - 47
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SP  - 1-24
EP  - 1-24
PB  - WILEY-V C H VERLAG GMBH
SN  - 16159306
KW  - Capillary electrophoresis
KW  - In-capillary reaction
KW  - High-throughput screening technology
KW  - Transverse diffusion of laminar flow profiles methodology
KW  - Drug
UR  - https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/epdf/10.1002/jssc.202400157
N2  - CE is a powerful separation technique offering quick and efficient analyses in various fields of bioanalytical chemistry. It is characterised by many well-known advantages, but one, which is perhaps the most important for this application field, is somewhat overlooked. It is the possibility of performing chemical and biochemical reactions at the nL scale inside the separation capillary. There are two basic formats applicable for this purpose, heterogeneous and homogeneous. In the former, one reactant is immobilised onto a particle or monolithic support or directly on the capillary wall, and the other is injected. In the latter, the reactant mixing inside a capillary is based on electromigration or diffusion. One of the diffusion-based methodologies, termed Transverse Diffusion of Laminar Flow Profiles, is the subject of this review. Since most studies utilising in-capillary reactions in CE focus on enzymes, which are being continuously and exhaustively reviewed, this review covers the atypical applications of this methodology, but still in the bioanalytical field. As can be seen from the demonstrated applications, they are not limited to reactions, but can also be utilised for other biochemical systems such as affinity interaction studies or biochemically important processes such as cell lysis, even in single-cell format.
ER  -

BRŽEZICKÁ, Taťána, Lenka KOHÚTOVÁ and Zdeněk GLATZ. Atypical applications of transverse diffusion of laminar flow profiles methodology for in-capillary reactions in capillary electrophoresis. \textit{JOURNAL OF SEPARATION SCIENCE}. GERMANY: WILEY-V C H VERLAG GMBH, 2024, vol.~47, No~13, p.~1-24. ISSN~1615-9306. Available from: https://dx.doi.org/10.1002/jssc.202400157.

Detailed Information on Publication Record