An extensive immunohistochemical analysis of 290 ovarian adult
granulosa cell tumors with 29 markers

J 2024

An extensive immunohistochemical analysis of 290 ovarian adult granulosa cell tumors with 29 markers

NEMEJCOVA, Kristyna, Adam SAFANDA, Michaela KENDALL BARTU, Romana MICHALKOVA, Marian SVAJDLER et. al.

Basic information

Original name

An extensive immunohistochemical analysis of 290 ovarian adult granulosa cell tumors with 29 markers

Authors

NEMEJCOVA, Kristyna (203 Czech Republic), Adam SAFANDA (203 Czech Republic), Michaela KENDALL BARTU (203 Czech Republic), Romana MICHALKOVA (203 Czech Republic), Marian SVAJDLER (203 Czech Republic), Tetiana SHATOKHINA (804 Ukraine), Jan LACO (203 Czech Republic), Radoslav MATEJ (203 Czech Republic), Gabor MEHES (348 Hungary), Jana DROZENOVA (203 Czech Republic), Jitka HAUSNEROVÁ (203 Czech Republic, belonging to the institution), Zuzana SPURKOVA (203 Czech Republic), Monika NÁLEŽINSKÁ (203 Czech Republic, belonging to the institution) and Pavel DUNDR (203 Czech Republic)

Edition

Virchows Archiv, New York, Springer, 2024, 0945-6317

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30109 Pathology

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 3.500 in 2022

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.1007/s00428-024-03854-0

UT WoS

001251527400001

Keywords in English

Ovarian tumors; Sex cord-stromal tumors; Granulosa cell tumors; Immunohistochemistry

Abstract

V originále

The current knowledge about the immunohistochemical features of adult granulosa cell tumor (AGCT) is mostly limited to the "traditional" immunohistochemical markers of sex cord differentiation, such as inhibin, calretinin, FOXL2, SF1, and CD99. Knowledge about the immunohistochemical markers possibly used for predictive purpose is limited. In our study, we focused on the immunohistochemical examination of 290 cases of AGCT classified based on strict diagnostic criteria, including molecular testing. The antibodies used included 12 of the "diagnostic" antibodies already examined in previous studies, 10 antibodies whose expression has not yet been examined in AGCT, and 7 antibodies with possible predictive significance, including the expression of HER2, PD-L1, CTLA4, and 4 mismatch repair (MMR) proteins. The results of our study showed expression of FOXL2, SF1, CD99, inhibin A, calretinin, ER, PR, AR, CKAE1/3, and CAIX in 98%, 100%, 90%, 78%, 45%, 41%, 94%, 82%, 26%, and 9% of AGCT, respectively. GATA3, SATB2, napsin A, MUC4, TTF1, and CD44 were all negative. PTEN showed a loss of expression in 71% of cases and DPC4 in 4% of cases. The aberrant staining pattern (overexpression) of p53 was found in 1% (3/268) of cases, 2 primary tumors, and 1 recurrent case. Concerning the predictive markers, the results of our study showed that AGCT is microsatellite stable, do not express PD-L1, and are HER2 negative. The CTLA4 expression was found in almost 70% of AGCT tumor cells.

Links

LM2023033, research and development project

Name: Síť českých biobank

Investor: Ministry of Education, Youth and Sports of the CR

Citovat

NEMEJCOVA, Kristyna, Adam SAFANDA, Michaela KENDALL BARTU, Romana MICHALKOVA, Marian SVAJDLER, Tetiana SHATOKHINA, Jan LACO, Radoslav MATEJ, Gabor MEHES, Jana DROZENOVA, Jitka HAUSNEROVÁ, Zuzana SPURKOVA, Monika NÁLEŽINSKÁ and Pavel DUNDR. An extensive immunohistochemical analysis of 290 ovarian adult granulosa cell tumors with 29 markers. Virchows Archiv. New York: Springer, 2024, vol. 485, No 3, p. 427-437. ISSN 0945-6317. Available from: https://dx.doi.org/10.1007/s00428-024-03854-0.

@article{2416237,
   author = {Nemejcova, Kristyna and Safanda, Adam and Kendall Bartu, Michaela and Michalkova, Romana and Svajdler, Marian and Shatokhina, Tetiana and Laco, Jan and Matej, Radoslav and Mehes, Gabor and Drozenova, Jana and Hausnerová, Jitka and Spurkova, Zuzana and Náležinská, Monika and Dundr, Pavel},
   article_location = {New York},
   article_number = {3},
   doi = {http://dx.doi.org/10.1007/s00428-024-03854-0},
   keywords = {Ovarian tumors; Sex cord-stromal tumors; Granulosa cell tumors; Immunohistochemistry},
   language = {eng},
   issn = {0945-6317},
   journal = {Virchows Archiv},
   title = {An extensive immunohistochemical analysis of 290 ovarian adult granulosa cell tumors with 29 markers},
   url = {https://link.springer.com/article/10.1007/s00428-024-03854-0},
   volume = {485},
   year = {2024}
}

TY  - JOUR
ID  - 2416237
AU  - Nemejcova, Kristyna - Safanda, Adam - Kendall Bartu, Michaela - Michalkova, Romana - Svajdler, Marian - Shatokhina, Tetiana - Laco, Jan - Matej, Radoslav - Mehes, Gabor - Drozenova, Jana - Hausnerová, Jitka - Spurkova, Zuzana - Náležinská, Monika - Dundr, Pavel
PY  - 2024
TI  - An extensive immunohistochemical analysis of 290 ovarian adult granulosa cell tumors with 29 markers
JF  - Virchows Archiv
VL  - 485
IS  - 3
SP  - 427-437
EP  - 427-437
PB  - Springer
SN  - 09456317
KW  - Ovarian tumors
KW  - Sex cord-stromal tumors
KW  - Granulosa cell tumors
KW  - Immunohistochemistry
UR  - https://link.springer.com/article/10.1007/s00428-024-03854-0
N2  - The current knowledge about the immunohistochemical features of adult granulosa cell tumor (AGCT) is mostly limited to the "traditional" immunohistochemical markers of sex cord differentiation, such as inhibin, calretinin, FOXL2, SF1, and CD99. Knowledge about the immunohistochemical markers possibly used for predictive purpose is limited. In our study, we focused on the immunohistochemical examination of 290 cases of AGCT classified based on strict diagnostic criteria, including molecular testing. The antibodies used included 12 of the "diagnostic" antibodies already examined in previous studies, 10 antibodies whose expression has not yet been examined in AGCT, and 7 antibodies with possible predictive significance, including the expression of HER2, PD-L1, CTLA4, and 4 mismatch repair (MMR) proteins. The results of our study showed expression of FOXL2, SF1, CD99, inhibin A, calretinin, ER, PR, AR, CKAE1/3, and CAIX in 98%, 100%, 90%, 78%, 45%, 41%, 94%, 82%, 26%, and 9% of AGCT, respectively. GATA3, SATB2, napsin A, MUC4, TTF1, and CD44 were all negative. PTEN showed a loss of expression in 71% of cases and DPC4 in 4% of cases. The aberrant staining pattern (overexpression) of p53 was found in 1% (3/268) of cases, 2 primary tumors, and 1 recurrent case. Concerning the predictive markers, the results of our study showed that AGCT is microsatellite stable, do not express PD-L1, and are HER2 negative. The CTLA4 expression was found in almost 70% of AGCT tumor cells.
ER  -

NEMEJCOVA, Kristyna, Adam SAFANDA, Michaela KENDALL BARTU, Romana MICHALKOVA, Marian SVAJDLER, Tetiana SHATOKHINA, Jan LACO, Radoslav MATEJ, Gabor MEHES, Jana DROZENOVA, Jitka HAUSNEROVÁ, Zuzana SPURKOVA, Monika NÁLEŽINSKÁ and Pavel DUNDR. An extensive immunohistochemical analysis of 290 ovarian adult granulosa cell tumors with 29 markers. \textit{Virchows Archiv}. New York: Springer, 2024, vol.~485, No~3, p.~427-437. ISSN~0945-6317. Available from: https://dx.doi.org/10.1007/s00428-024-03854-0.

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