Dynamics of Cellular Regulation of Fractalkine/CX3CL1 and Its
Receptor CX3CR1 in the Rat Trigeminal Subnucleus Caudalis after
Unilateral Infraorbital Nerve Lesion-Extended Cellular
Signaling of the CX3CL1/CX3CR1 Axis in the Development of
Trigeminal Neuropathic Pain

J 2024

Dynamics of Cellular Regulation of Fractalkine/CX3CL1 and Its Receptor CX3CR1 in the Rat Trigeminal Subnucleus Caudalis after Unilateral Infraorbital Nerve Lesion-Extended Cellular Signaling of the CX3CL1/CX3CR1 Axis in the Development of Trigeminal Neuropathic Pain

KUBÍČKOVÁ, Lucie and Petr DUBOVÝ

Basic information

Original name

Dynamics of Cellular Regulation of Fractalkine/CX3CL1 and Its Receptor CX3CR1 in the Rat Trigeminal Subnucleus Caudalis after Unilateral Infraorbital Nerve Lesion-Extended Cellular Signaling of the CX3CL1/CX3CR1 Axis in the Development of Trigeminal Neuropathic Pain

Authors

KUBÍČKOVÁ, Lucie (203 Czech Republic, belonging to the institution) and Petr DUBOVÝ (203 Czech Republic, belonging to the institution)

Edition

International Journal of Molecular Sciences, BASEL, MDPI, 2024, 1661-6596

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10608 Biochemistry and molecular biology

Country of publisher

Switzerland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 5.600 in 2022

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.3390/ijms25116069

UT WoS

001246868600001

Keywords in English

neurons; reactive astroglia; microglial cells; chemokines; chemokine receptors; immunohistochemistry; image analysis

Abstract

V originále

The cellular distribution and changes in CX3CL1/fractalkine and its receptor CX3CR1 protein levels in the trigeminal subnucleus caudalis (TSC) of rats with unilateral infraorbital nerve ligation (IONL) were investigated on postoperation days 1, 3, 7, and 14 (POD1, POD3, POD7, and POD14, respectively) and compared with those of sham-operated and na & iuml;ve controls. Behavioral tests revealed a significant increase in tactile hypersensitivity bilaterally in the vibrissal pads of both sham- and IONL-operated animals from POD1 to POD7, with a trend towards normalization in sham controls at POD14. Image analysis revealed increased CX3CL1 immunofluorescence (IF) intensities bilaterally in the TSC neurons of both sham- and IONL-operated rats at all survival periods. Reactive astrocytes in the ipsilateral TSC also displayed CX3CL1-IF from POD3 to POD14. At POD1 and POD3, microglial cells showed high levels of CX3CR1-IF, which decreased by POD7 and POD14. Conversely, CX3CR1 was increased in TSC neurons and reactive astrocytes at POD7 and POD14, which coincided with high levels of CX3CL1-IF and ADAM17-IF. This indicates that CX3CL1/CX3CR1 may be involved in reciprocal signaling between TSC neurons and reactive astrocytes. The level of CatS-IF in microglial cells suggests that soluble CX3CL1 may be involved in neuron-microglial cell signaling at POD3 and POD7, while ADAM17 allows this release at all studied time points. These results indicate an extended CX3CL1/CX3CR1 signaling axis and its role in the crosstalk between TSC neurons and glial cells during the development of trigeminal neuropathic pain.

Links

MUNI/A/1563/2023, interní kód MU

Name: Funkční morfologie: od molekulární biologie ke klinické anatomii 3

Investor: Masaryk University

Citovat

KUBÍČKOVÁ, Lucie and Petr DUBOVÝ. Dynamics of Cellular Regulation of Fractalkine/CX3CL1 and Its Receptor CX3CR1 in the Rat Trigeminal Subnucleus Caudalis after Unilateral Infraorbital Nerve Lesion-Extended Cellular Signaling of the CX3CL1/CX3CR1 Axis in the Development of Trigeminal Neuropathic Pain. International Journal of Molecular Sciences. BASEL: MDPI, 2024, vol. 25, No 11, p. 1-18. ISSN 1661-6596. Available from: https://dx.doi.org/10.3390/ijms25116069.

@article{2416242,
   author = {Kubíčková, Lucie and Dubový, Petr},
   article_location = {BASEL},
   article_number = {11},
   doi = {http://dx.doi.org/10.3390/ijms25116069},
   keywords = {neurons; reactive astroglia; microglial cells; chemokines; chemokine receptors; immunohistochemistry; image analysis},
   language = {eng},
   issn = {1661-6596},
   journal = {International Journal of Molecular Sciences},
   title = {Dynamics of Cellular Regulation of Fractalkine/CX3CL1 and Its Receptor CX3CR1 in the Rat Trigeminal Subnucleus Caudalis after Unilateral Infraorbital Nerve Lesion-Extended Cellular Signaling of the CX3CL1/CX3CR1 Axis in the Development of Trigeminal Neuropathic Pain},
   url = {https://www.mdpi.com/2072-6694/16/11/2084},
   volume = {25},
   year = {2024}
}

TY  - JOUR
ID  - 2416242
AU  - Kubíčková, Lucie - Dubový, Petr
PY  - 2024
TI  - Dynamics of Cellular Regulation of Fractalkine/CX3CL1 and Its Receptor CX3CR1 in the Rat Trigeminal Subnucleus Caudalis after Unilateral Infraorbital Nerve Lesion-Extended Cellular Signaling of the CX3CL1/CX3CR1 Axis in the Development of Trigeminal Neuropathic Pain
JF  - International Journal of Molecular Sciences
VL  - 25
IS  - 11
SP  - 1-18
EP  - 1-18
PB  - MDPI
SN  - 16616596
KW  - neurons
KW  - reactive astroglia
KW  - microglial cells
KW  - chemokines
KW  - chemokine receptors
KW  - immunohistochemistry
KW  - image analysis
UR  - https://www.mdpi.com/2072-6694/16/11/2084
N2  - The cellular distribution and changes in CX3CL1/fractalkine and its receptor CX3CR1 protein levels in the trigeminal subnucleus caudalis (TSC) of rats with unilateral infraorbital nerve ligation (IONL) were investigated on postoperation days 1, 3, 7, and 14 (POD1, POD3, POD7, and POD14, respectively) and compared with those of sham-operated and na & iuml;ve controls. Behavioral tests revealed a significant increase in tactile hypersensitivity bilaterally in the vibrissal pads of both sham- and IONL-operated animals from POD1 to POD7, with a trend towards normalization in sham controls at POD14. Image analysis revealed increased CX3CL1 immunofluorescence (IF) intensities bilaterally in the TSC neurons of both sham- and IONL-operated rats at all survival periods. Reactive astrocytes in the ipsilateral TSC also displayed CX3CL1-IF from POD3 to POD14. At POD1 and POD3, microglial cells showed high levels of CX3CR1-IF, which decreased by POD7 and POD14. Conversely, CX3CR1 was increased in TSC neurons and reactive astrocytes at POD7 and POD14, which coincided with high levels of CX3CL1-IF and ADAM17-IF. This indicates that CX3CL1/CX3CR1 may be involved in reciprocal signaling between TSC neurons and reactive astrocytes. The level of CatS-IF in microglial cells suggests that soluble CX3CL1 may be involved in neuron-microglial cell signaling at POD3 and POD7, while ADAM17 allows this release at all studied time points. These results indicate an extended CX3CL1/CX3CR1 signaling axis and its role in the crosstalk between TSC neurons and glial cells during the development of trigeminal neuropathic pain.
ER  -

KUBÍČKOVÁ, Lucie and Petr DUBOVÝ. Dynamics of Cellular Regulation of Fractalkine/CX3CL1 and Its Receptor CX3CR1 in the Rat Trigeminal Subnucleus Caudalis after Unilateral Infraorbital Nerve Lesion-Extended Cellular Signaling of the CX3CL1/CX3CR1 Axis in the Development of Trigeminal Neuropathic Pain. \textit{International Journal of Molecular Sciences}. BASEL: MDPI, 2024, vol.~25, No~11, p.~1-18. ISSN~1661-6596. Available from: https://dx.doi.org/10.3390/ijms25116069.

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