The impact of neurodegeneration on the electrical activity of brain tissue: multielectrode array analysis

ANGELOVSKI, Andrijana, Barbora LIŠČÁKOVÁ, Olga ŠVECOVÁ, Hana HŘÍBKOVÁ, Jiří SEDMÍK, Dáša BOHAČIAKOVÁ, Petr KLIMEŠ, Martina KOLAJOVÁ, Milan BRÁZDIL and Markéta BÉBAROVÁ. The impact of neurodegeneration on the electrical activity of brain tissue: multielectrode array analysis. In FENS Forum 2024. 2024.

Basic information

• Original name: The impact of neurodegeneration on the electrical activity of brain tissue: multielectrode array analysis
• Authors: ANGELOVSKI, Andrijana, Barbora LIŠČÁKOVÁ, Olga ŠVECOVÁ, Hana HŘÍBKOVÁ, Jiří SEDMÍK, Dáša BOHAČIAKOVÁ, Petr KLIMEŠ, Martina KOLAJOVÁ, Milan BRÁZDIL and Markéta BÉBAROVÁ.
• Edition: FENS Forum 2024, 2024.

Other information

• Original language: English
• Type of outcome: Conference abstract
• Country of publisher: Austria
• Confidentiality degree: is not subject to a state or trade secret
• Organization unit: Faculty of Medicine
• Keywords in English: cerebral organoids, Alzheimer disease, electrical activity, multielectrode array analysis
• Tags: International impact

Abstract

Alzheimer's disease (AD) is characterized by neurodegeneration due to an accumulation of abnormal amounts of neurodegenerative proteins (NP). Elevated levels of NP have also been observed in patients suffering from temporal lobe epilepsy (TLE) which brings neurodegeneration into association with an increased risk of epileptic seizures. This study aimed to provide pilot data on the possible relationship between excitability and neurodegeneration. Electrical activity was recorded at 37°C using the multielectrode array (MEA) technique in cerebral organoids prepared from induced pluripotent stem cells (iPSCs) derived from a patient with the familial form of AD (nAD = 6) and an unrelated healthy subject (nWT = 5). The study found a significantly increased number of active electrodes, showing both spikes and bursts, in AD organoids compared to healthy controls (e.g. differentiation day D105: WT 3.39 vs. AD 20.34% in the case of spikes, P < 0.01, and WT 1.7 vs. AD 17.0 % in the case of bursts, P < 0.05). On some days, a significant increase was also observed in the case of burst duration (D118; P < 0.05), burst count (D105; P < 0.05), or intraburst spike number (D114; P < 0.05). These pilot data indicate that neurodegenerative cerebral organoids show signs of heightened neural tissue excitability compared to healthy ones. Our ongoing analysis aims to validate these pilot results further and incorporate data describing the content of NP in these cerebral organoids for establishing an association between neurodegeneration and increased neuronal excitability.

Links

• GA24-12028S, research and development project: Name: Atlas Alzheimerovy choroby: Mapování vývoje demence u cerebrálních organoidů pomocí single-cell transkriptomiky

Investor: Czech Science Foundation, Constructing the Developmental Atlas of Alzheimer’s Disease using Cerebral Organoids and Single-Cell Transcriptomics

• LX22NPO5107, research and development project: Name: Národní ústav pro neurologický výzkum

Investor: Ministry of Education, Youth and Sports of the CR, 5.1 EXCELES

• MUNI/A/1547/2023, interní kód MU: Name: Analýza (dys)funkce: od molekul k živému organismu

Investor: Masaryk University, Analysis of (dys)function: from molecules to the living organism

• NU22-04-00366, research and development project: Name: Role neurodegenerace v patogenezi, manifestaci a prognóze MTLE/HS - in vivo, ex vivo a in vitro perspektiva

Investor: Ministry of Health of the CR, The role of neurodegeneration in pathogenesis, manifestations and prognosis of MTLE/HS - in vivo, ex vivo and in vitro perspective, Subprogram 1 - standard