Detailed Information on Publication Record
2024
The impact of neurodegeneration on the electrical activity of brain tissue: multielectrode array analysis
ANGELOVSKI, Andrijana, Barbora LIŠČÁKOVÁ, Olga ŠVECOVÁ, Hana HŘÍBKOVÁ, Jiří SEDMÍK et. al.Basic information
Original name
The impact of neurodegeneration on the electrical activity of brain tissue: multielectrode array analysis
Authors
ANGELOVSKI, Andrijana (688 Serbia, belonging to the institution), Barbora LIŠČÁKOVÁ (703 Slovakia, belonging to the institution), Olga ŠVECOVÁ (203 Czech Republic, belonging to the institution), Hana HŘÍBKOVÁ (203 Czech Republic, belonging to the institution), Jiří SEDMÍK (203 Czech Republic, belonging to the institution), Dáša BOHAČIAKOVÁ (703 Slovakia, belonging to the institution), Petr KLIMEŠ (203 Czech Republic), Martina KOLAJOVÁ, Milan BRÁZDIL (203 Czech Republic) and Markéta BÉBAROVÁ (203 Czech Republic, guarantor, belonging to the institution)
Edition
FENS Forum 2024, 2024
Other information
Language
English
Type of outcome
Konferenční abstrakt
Field of Study
30210 Clinical neurology
Country of publisher
Austria
Confidentiality degree
není předmětem státního či obchodního tajemství
Organization unit
Faculty of Medicine
Keywords in English
cerebral organoids; Alzheimer disease; electrical activity; multielectrode array analysis
Tags
International impact
Změněno: 19/8/2024 14:19, Mgr. Tereza Miškechová
Abstract
V originále
Alzheimer's disease (AD) is characterized by neurodegeneration due to an accumulation of abnormal amounts of neurodegenerative proteins (NP). Elevated levels of NP have also been observed in patients suffering from temporal lobe epilepsy (TLE) which brings neurodegeneration into association with an increased risk of epileptic seizures. This study aimed to provide pilot data on the possible relationship between excitability and neurodegeneration. Electrical activity was recorded at 37°C using the multielectrode array (MEA) technique in cerebral organoids prepared from induced pluripotent stem cells (iPSCs) derived from a patient with the familial form of AD (nAD = 6) and an unrelated healthy subject (nWT = 5). The study found a significantly increased number of active electrodes, showing both spikes and bursts, in AD organoids compared to healthy controls (e.g. differentiation day D105: WT 3.39 vs. AD 20.34% in the case of spikes, P < 0.01, and WT 1.7 vs. AD 17.0 % in the case of bursts, P < 0.05). On some days, a significant increase was also observed in the case of burst duration (D118; P < 0.05), burst count (D105; P < 0.05), or intraburst spike number (D114; P < 0.05). These pilot data indicate that neurodegenerative cerebral organoids show signs of heightened neural tissue excitability compared to healthy ones. Our ongoing analysis aims to validate these pilot results further and incorporate data describing the content of NP in these cerebral organoids for establishing an association between neurodegeneration and increased neuronal excitability.
Links
| GA24-12028S, research and development project |
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| LX22NPO5107, research and development project |
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| MUNI/A/1547/2023, interní kód MU |
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| NU22-04-00366, research and development project |
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