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@article{2417663,
author = {Knoz, Martin and Holoubek, Jakub and Lipový, Břetislav and Faldyna, Martin and Chaloupková, Radka and Pavlinakova, Veronika and Muchova, Johana and Brtnikova, Jana and Jarkovský, Jiří and Vojtova, Lucy},
article_location = {London},
article_number = {6},
doi = {http://dx.doi.org/10.1016/j.burns.2024.03.020},
keywords = {Dermal substitute; Cutometry; Fibroblast grow factor 2; Scar; Visual assessment; Animal experiment},
language = {eng},
issn = {0305-4179},
journal = {Burns},
title = {Evaluation of viscoelastic parameters and photo-based assessment of newly developed dermal substitutes modified with thermostabilized fibroblast growth factor 2},
url = {https://www.sciencedirect.com/science/article/pii/S0305417924000925?via%3Dihub},
volume = {50},
year = {2024}
}
TY - JOUR
ID - 2417663
AU - Knoz, Martin - Holoubek, Jakub - Lipový, Břetislav - Faldyna, Martin - Chaloupková, Radka - Pavlinakova, Veronika - Muchova, Johana - Brtnikova, Jana - Jarkovský, Jiří - Vojtova, Lucy
PY - 2024
TI - Evaluation of viscoelastic parameters and photo-based assessment of newly developed dermal substitutes modified with thermostabilized fibroblast growth factor 2
JF - Burns
VL - 50
IS - 6
SP - 1586-1596
EP - 1586-1596
PB - ELSEVIER SCI LTD
SN - 03054179
KW - Dermal substitute
KW - Cutometry
KW - Fibroblast grow factor 2
KW - Scar
KW - Visual assessment
KW - Animal experiment
UR - https://www.sciencedirect.com/science/article/pii/S0305417924000925?via%3Dihub
N2 - Background: The purpose of dermal substitutes is to mimic the basic properties of the extracellular matrix of human skin. The application of dermal substitutes to the defect reduces the formation of hypertrophic scars and improves the scar quality. This study aims to develop an original dermal substitute enriched with stable fibroblast growth factor 2 (FGF2-STAB (R)) and test it in an animal model. Methods: Dermal substitutes based on collagen/chitosan scaffolds or collagen/chitosan scaffolds with nanofibrous layer were prepared and enriched with FGF2-STAB (R) at concentrations of 0, 0.1, 1.0, and 10.0 mu g.cm(-2). The performance of these dermal substitutes was tested in vivo on artificially formed skin defects in female swine. The outcomes were evaluated using cutometry at 3 and 6 months. In addition, visual appearance was assessed based on photos of the scars at 1-month, 3-month and 6-month follow-ups using Yeong scale and Visual Analog Scale. Results: The dermal substitute was fully integrated into all defects and all wounds healed successfully. FGF2-STAB (R)-enriched matrices yielded better results in cutometry compared to scaffolds without FGF2. Visual evaluation at 1, 3, and 6 months follow-ups detected no significant differences among groups. The FGF2-STAB (R) effectiveness in improving the elasticity of scar tissues was confirmed in the swine model. This effect was independently observed in the scaffolds with nanofibres as well as in the scaffolds without nanofibres. Conclusion: The formation of scars with the best elasticity was exhibited by addition 1.0 mu g.cm(-2) of FGF2-STAB (R) into the scaffolds, although it had no significant effect on visual appearance at longer follow-ups. This study creates the basis for further translational studies of the developed product and its progression into the clinical phase of the research.
ER -
KNOZ, Martin, Jakub HOLOUBEK, Břetislav LIPOVÝ, Martin FALDYNA, Radka CHALOUPKOVÁ, Veronika PAVLINAKOVA, Johana MUCHOVA, Jana BRTNIKOVA, Jiří JARKOVSKÝ and Lucy VOJTOVA. Evaluation of viscoelastic parameters and photo-based assessment of newly developed dermal substitutes modified with thermostabilized fibroblast growth factor 2. \textit{Burns}. London: ELSEVIER SCI LTD, 2024, vol.~50, No~6, p.~1586-1596. ISSN~0305-4179. Available from: https://dx.doi.org/10.1016/j.burns.2024.03.020.
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