Evaluation of viscoelastic parameters and photo-based
assessment of newly developed dermal substitutes modified with
thermostabilized fibroblast growth factor 2

J 2024

Evaluation of viscoelastic parameters and photo-based assessment of newly developed dermal substitutes modified with thermostabilized fibroblast growth factor 2

KNOZ, Martin, Jakub HOLOUBEK, Břetislav LIPOVÝ, Martin FALDYNA, Radka CHALOUPKOVÁ et. al.

Basic information

Original name

Evaluation of viscoelastic parameters and photo-based assessment of newly developed dermal substitutes modified with thermostabilized fibroblast growth factor 2

Authors

KNOZ, Martin (203 Czech Republic, belonging to the institution), Jakub HOLOUBEK (203 Czech Republic, belonging to the institution), Břetislav LIPOVÝ (203 Czech Republic, belonging to the institution), Martin FALDYNA (203 Czech Republic), Radka CHALOUPKOVÁ (203 Czech Republic), Veronika PAVLINAKOVA (203 Czech Republic), Johana MUCHOVA (203 Czech Republic), Jana BRTNIKOVA (203 Czech Republic), Jiří JARKOVSKÝ (203 Czech Republic, belonging to the institution) and Lucy VOJTOVA (203 Czech Republic)

Edition

Burns, London, ELSEVIER SCI LTD, 2024, 0305-4179

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30212 Surgery

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 2.700 in 2022

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.1016/j.burns.2024.03.020

UT WoS

001252447100001

Keywords in English

Dermal substitute; Cutometry; Fibroblast grow factor 2; Scar; Visual assessment; Animal experiment

Abstract

V originále

Background: The purpose of dermal substitutes is to mimic the basic properties of the extracellular matrix of human skin. The application of dermal substitutes to the defect reduces the formation of hypertrophic scars and improves the scar quality. This study aims to develop an original dermal substitute enriched with stable fibroblast growth factor 2 (FGF2-STAB (R)) and test it in an animal model. Methods: Dermal substitutes based on collagen/chitosan scaffolds or collagen/chitosan scaffolds with nanofibrous layer were prepared and enriched with FGF2-STAB (R) at concentrations of 0, 0.1, 1.0, and 10.0 mu g.cm(-2). The performance of these dermal substitutes was tested in vivo on artificially formed skin defects in female swine. The outcomes were evaluated using cutometry at 3 and 6 months. In addition, visual appearance was assessed based on photos of the scars at 1-month, 3-month and 6-month follow-ups using Yeong scale and Visual Analog Scale. Results: The dermal substitute was fully integrated into all defects and all wounds healed successfully. FGF2-STAB (R)-enriched matrices yielded better results in cutometry compared to scaffolds without FGF2. Visual evaluation at 1, 3, and 6 months follow-ups detected no significant differences among groups. The FGF2-STAB (R) effectiveness in improving the elasticity of scar tissues was confirmed in the swine model. This effect was independently observed in the scaffolds with nanofibres as well as in the scaffolds without nanofibres. Conclusion: The formation of scars with the best elasticity was exhibited by addition 1.0 mu g.cm(-2) of FGF2-STAB (R) into the scaffolds, although it had no significant effect on visual appearance at longer follow-ups. This study creates the basis for further translational studies of the developed product and its progression into the clinical phase of the research.

Links

NU22-08-00454, research and development project

Name: Biomimetický vícevrstevný buněčný nosič čtvrté generace pro jednokrokovou úplnou náhradu kůže: z laboratoře do klinické aplikace

Investor: Ministry of Health of the CR, Subprogram 1 - standard

90251, large research infrastructures

Name: CzechNanoLab II

Citovat

KNOZ, Martin, Jakub HOLOUBEK, Břetislav LIPOVÝ, Martin FALDYNA, Radka CHALOUPKOVÁ, Veronika PAVLINAKOVA, Johana MUCHOVA, Jana BRTNIKOVA, Jiří JARKOVSKÝ and Lucy VOJTOVA. Evaluation of viscoelastic parameters and photo-based assessment of newly developed dermal substitutes modified with thermostabilized fibroblast growth factor 2. Burns. London: ELSEVIER SCI LTD, 2024, vol. 50, No 6, p. 1586-1596. ISSN 0305-4179. Available from: https://dx.doi.org/10.1016/j.burns.2024.03.020.

@article{2417663,
   author = {Knoz, Martin and Holoubek, Jakub and Lipový, Břetislav and Faldyna, Martin and Chaloupková, Radka and Pavlinakova, Veronika and Muchova, Johana and Brtnikova, Jana and Jarkovský, Jiří and Vojtova, Lucy},
   article_location = {London},
   article_number = {6},
   doi = {http://dx.doi.org/10.1016/j.burns.2024.03.020},
   keywords = {Dermal substitute; Cutometry; Fibroblast grow factor 2; Scar; Visual assessment; Animal experiment},
   language = {eng},
   issn = {0305-4179},
   journal = {Burns},
   title = {Evaluation of viscoelastic parameters and photo-based assessment of newly developed dermal substitutes modified with thermostabilized fibroblast growth factor 2},
   url = {https://www.sciencedirect.com/science/article/pii/S0305417924000925?via%3Dihub},
   volume = {50},
   year = {2024}
}

TY  - JOUR
ID  - 2417663
AU  - Knoz, Martin - Holoubek, Jakub - Lipový, Břetislav - Faldyna, Martin - Chaloupková, Radka - Pavlinakova, Veronika - Muchova, Johana - Brtnikova, Jana - Jarkovský, Jiří - Vojtova, Lucy
PY  - 2024
TI  - Evaluation of viscoelastic parameters and photo-based assessment of newly developed dermal substitutes modified with thermostabilized fibroblast growth factor 2
JF  - Burns
VL  - 50
IS  - 6
SP  - 1586-1596
EP  - 1586-1596
PB  - ELSEVIER SCI LTD
SN  - 03054179
KW  - Dermal substitute
KW  - Cutometry
KW  - Fibroblast grow factor 2
KW  - Scar
KW  - Visual assessment
KW  - Animal experiment
UR  - https://www.sciencedirect.com/science/article/pii/S0305417924000925?via%3Dihub
N2  - Background: The purpose of dermal substitutes is to mimic the basic properties of the extracellular matrix of human skin. The application of dermal substitutes to the defect reduces the formation of hypertrophic scars and improves the scar quality. This study aims to develop an original dermal substitute enriched with stable fibroblast growth factor 2 (FGF2-STAB (R)) and test it in an animal model. Methods: Dermal substitutes based on collagen/chitosan scaffolds or collagen/chitosan scaffolds with nanofibrous layer were prepared and enriched with FGF2-STAB (R) at concentrations of 0, 0.1, 1.0, and 10.0 mu g.cm(-2). The performance of these dermal substitutes was tested in vivo on artificially formed skin defects in female swine. The outcomes were evaluated using cutometry at 3 and 6 months. In addition, visual appearance was assessed based on photos of the scars at 1-month, 3-month and 6-month follow-ups using Yeong scale and Visual Analog Scale. Results: The dermal substitute was fully integrated into all defects and all wounds healed successfully. FGF2-STAB (R)-enriched matrices yielded better results in cutometry compared to scaffolds without FGF2. Visual evaluation at 1, 3, and 6 months follow-ups detected no significant differences among groups. The FGF2-STAB (R) effectiveness in improving the elasticity of scar tissues was confirmed in the swine model. This effect was independently observed in the scaffolds with nanofibres as well as in the scaffolds without nanofibres. Conclusion: The formation of scars with the best elasticity was exhibited by addition 1.0 mu g.cm(-2) of FGF2-STAB (R) into the scaffolds, although it had no significant effect on visual appearance at longer follow-ups. This study creates the basis for further translational studies of the developed product and its progression into the clinical phase of the research.
ER  -

KNOZ, Martin, Jakub HOLOUBEK, Břetislav LIPOVÝ, Martin FALDYNA, Radka CHALOUPKOVÁ, Veronika PAVLINAKOVA, Johana MUCHOVA, Jana BRTNIKOVA, Jiří JARKOVSKÝ and Lucy VOJTOVA. Evaluation of viscoelastic parameters and photo-based assessment of newly developed dermal substitutes modified with thermostabilized fibroblast growth factor 2. \textit{Burns}. London: ELSEVIER SCI LTD, 2024, vol.~50, No~6, p.~1586-1596. ISSN~0305-4179. Available from: https://dx.doi.org/10.1016/j.burns.2024.03.020.

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