Detailed Information on Publication Record
2024
Single-molecule microfluidic assay for prostate-specific antigen based on magnetic beads and upconversion nanoparticles
SKLENÁROVÁ, Dorota, Antonín HLAVÁČEK, Jana KŘIVÁNKOVÁ, Julian BRANDMEIER, Julie WEISOVÁ et. al.Basic information
Original name
Single-molecule microfluidic assay for prostate-specific antigen based on magnetic beads and upconversion nanoparticles
Authors
SKLENÁROVÁ, Dorota (703 Slovakia, belonging to the institution), Antonín HLAVÁČEK (203 Czech Republic), Jana KŘIVÁNKOVÁ (203 Czech Republic), Julian BRANDMEIER (276 Germany, belonging to the institution), Julie WEISOVÁ, Michal ŘIHÁČEK (203 Czech Republic, belonging to the institution), Hans-Heiner GORRIS (276 Germany, belonging to the institution), Petr SKLÁDAL (203 Czech Republic, belonging to the institution) and Zdeněk FARKA (203 Czech Republic, guarantor, belonging to the institution)
Edition
Lab on a Chip, Cambridge, Royal Society of Chemistry, 2024, 1473-0197
Other information
Language
English
Type of outcome
Článek v odborném periodiku
Field of Study
10406 Analytical chemistry
Country of publisher
United Kingdom of Great Britain and Northern Ireland
Confidentiality degree
není předmětem státního či obchodního tajemství
References:
Impact factor
Impact factor: 6.100 in 2022
Organization unit
Faculty of Science
UT WoS
001258448900001
Keywords in English
Photon-upconversion nanoparticle; Immunoassay; Microfluidics; Prostate-specific antigen
Tags
Tags
International impact, Reviewed
Změněno: 5/8/2024 13:22, Mgr. Tereza Miškechová
Abstract
V originále
Early-stage diagnosis of prostatic carcinoma is essential for successful treatment and, thus, significant prognosis improvement. In laboratory practice, the standard non-invasive diagnostic approach is the immunochemical detection of the associated biomarker, prostate-specific antigen (PSA). Ultrasensitive detection of PSA is essential for both diagnostic and recurrence monitoring purposes. To achieve exceptional sensitivity, we have developed a microfluidic device with a flow-through cell for single-molecule analysis using photon-upconversion nanoparticles (UCNPs) as a detection label. For this purpose, magnetic microparticles (MBs) were first optimized for the capture and preconcentration of PSA and then used to implement a bead-based upconversion-linked immunoassay (ULISA) in the microfluidic device. The digital readout based on counting single nanoparticle-labeled PSA molecules on MBs enabled a detection limit of 1.04 pg mL−1 (36 fM) in 50% fetal bovine serum, which is an 11-fold improvement over the respective analog MB-based ULISA. The microfluidic technique conferred several other advantages, such as easy implementation and the potential for achieving high-throughput analysis. Finally, it was proven that the microfluidic setup is suitable for clinical sample analysis, showing a good correlation with a reference electrochemiluminescence assay (recovery rates between 97% and 105%).
Links
| GA21-03156S, research and development project |
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