Single-molecule microfluidic assay for prostate-specific antigen based on magnetic beads and upconversion nanoparticles

SKLENÁROVÁ, Dorota, Antonín HLAVÁČEK, Jana KŘIVÁNKOVÁ, Julian BRANDMEIER, Julie WEISOVÁ, Michal ŘIHÁČEK, Hans-Heiner GORRIS, Petr SKLÁDAL and Zdeněk FARKA. Single-molecule microfluidic assay for prostate-specific antigen based on magnetic beads and upconversion nanoparticles. Lab on a Chip. Royal Society of Chemistry, 2024, vol. 24, 24 Jun 2024, p. 3536-3545. ISSN 1473-0197. Available from: https://dx.doi.org/10.1039/D4LC00346B.

Basic information

• Original name: Single-molecule microfluidic assay for prostate-specific antigen based on magnetic beads and upconversion nanoparticles
• Authors: SKLENÁROVÁ, Dorota (703 Slovakia, belonging to the institution), Antonín HLAVÁČEK (203 Czech Republic), Jana KŘIVÁNKOVÁ (203 Czech Republic), Julian BRANDMEIER (276 Germany, belonging to the institution), Julie WEISOVÁ, Michal ŘIHÁČEK (203 Czech Republic, belonging to the institution), Hans-Heiner GORRIS (276 Germany, belonging to the institution), Petr SKLÁDAL (203 Czech Republic, belonging to the institution) and Zdeněk FARKA (203 Czech Republic, guarantor, belonging to the institution).
• Edition: Lab on a Chip, Royal Society of Chemistry, 2024, 1473-0197.

Other information

• Original language: English
• Type of outcome: Article in a journal
• Field of Study: 10406 Analytical chemistry
• Country of publisher: United Kingdom of Great Britain and Northern Ireland
• Confidentiality degree: is not subject to a state or trade secret
• WWW: URL
• Impact factor: Impact factor: 6.100 in 2022
• Organization unit: Faculty of Science
• Doi: http://dx.doi.org/10.1039/D4LC00346B
• UT WoS: 001258448900001
• Keywords in English: Photon-upconversion nanoparticle; Immunoassay; Microfluidics; Prostate-specific antigen
• Tags: 14110616
• Tags: International impact, Reviewed

Abstract

Early-stage diagnosis of prostatic carcinoma is essential for successful treatment and, thus, significant prognosis improvement. In laboratory practice, the standard non-invasive diagnostic approach is the immunochemical detection of the associated biomarker, prostate-specific antigen (PSA). Ultrasensitive detection of PSA is essential for both diagnostic and recurrence monitoring purposes. To achieve exceptional sensitivity, we have developed a microfluidic device with a flow-through cell for single-molecule analysis using photon-upconversion nanoparticles (UCNPs) as a detection label. For this purpose, magnetic microparticles (MBs) were first optimized for the capture and preconcentration of PSA and then used to implement a bead-based upconversion-linked immunoassay (ULISA) in the microfluidic device. The digital readout based on counting single nanoparticle-labeled PSA molecules on MBs enabled a detection limit of 1.04 pg mL−1 (36 fM) in 50% fetal bovine serum, which is an 11-fold improvement over the respective analog MB-based ULISA. The microfluidic technique conferred several other advantages, such as easy implementation and the potential for achieving high-throughput analysis. Finally, it was proven that the microfluidic setup is suitable for clinical sample analysis, showing a good correlation with a reference electrochemiluminescence assay (recovery rates between 97% and 105%).

Links

• GA21-03156S, research and development project: Name: Foton-upkonverzní značky pro mikrofluidní jednomolekulové imunostanovení proteinových biomarkerů

Investor: Czech Science Foundation