Single-molecule microfluidic assay for prostate-specific
antigen based on magnetic beads and upconversion nanoparticles

J 2024

Single-molecule microfluidic assay for prostate-specific antigen based on magnetic beads and upconversion nanoparticles

SKLENÁROVÁ, Dorota; Antonín HLAVÁČEK; Jana KŘIVÁNKOVÁ; Julian BRANDMEIER; Julie WEISOVÁ et. al.

Basic information

Original name

Single-molecule microfluidic assay for prostate-specific antigen based on magnetic beads and upconversion nanoparticles

Authors

SKLENÁROVÁ, Dorota (703 Slovakia, belonging to the institution); Antonín HLAVÁČEK (203 Czech Republic); Jana KŘIVÁNKOVÁ (203 Czech Republic); Julian BRANDMEIER (276 Germany, belonging to the institution); Julie WEISOVÁ; Michal ŘIHÁČEK (203 Czech Republic, belonging to the institution); Hans-Heiner GORRIS (276 Germany, belonging to the institution); Petr SKLÁDAL (203 Czech Republic, belonging to the institution) and Zdeněk FARKA (203 Czech Republic, guarantor, belonging to the institution)

Edition

Lab on a Chip, Cambridge, Royal Society of Chemistry, 2024, 1473-0197

Other information

Language

English

Type of outcome

Article in a journal

Field of Study

10406 Analytical chemistry

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

is not subject to a state or trade secret

References:

URL

Impact factor

Impact factor: 6.100 in 2023

RIV identification code

RIV/00216224:14310/24:00139473

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.1039/D4LC00346B

UT WoS

001258448900001

EID Scopus

2-s2.0-85197932979

Keywords in English

Photon-upconversion nanoparticle; Immunoassay; Microfluidics; Prostate-specific antigen

Abstract

V originále

Early-stage diagnosis of prostatic carcinoma is essential for successful treatment and, thus, significant prognosis improvement. In laboratory practice, the standard non-invasive diagnostic approach is the immunochemical detection of the associated biomarker, prostate-specific antigen (PSA). Ultrasensitive detection of PSA is essential for both diagnostic and recurrence monitoring purposes. To achieve exceptional sensitivity, we have developed a microfluidic device with a flow-through cell for single-molecule analysis using photon-upconversion nanoparticles (UCNPs) as a detection label. For this purpose, magnetic microparticles (MBs) were first optimized for the capture and preconcentration of PSA and then used to implement a bead-based upconversion-linked immunoassay (ULISA) in the microfluidic device. The digital readout based on counting single nanoparticle-labeled PSA molecules on MBs enabled a detection limit of 1.04 pg mL−1 (36 fM) in 50% fetal bovine serum, which is an 11-fold improvement over the respective analog MB-based ULISA. The microfluidic technique conferred several other advantages, such as easy implementation and the potential for achieving high-throughput analysis. Finally, it was proven that the microfluidic setup is suitable for clinical sample analysis, showing a good correlation with a reference electrochemiluminescence assay (recovery rates between 97% and 105%).

Links

GA21-03156S, research and development project

Name: Foton-upkonverzní značky pro mikrofluidní jednomolekulové imunostanovení proteinových biomarkerů

Investor: Czech Science Foundation

Cite

SKLENÁROVÁ, Dorota; Antonín HLAVÁČEK; Jana KŘIVÁNKOVÁ; Julian BRANDMEIER; Julie WEISOVÁ; Michal ŘIHÁČEK; Hans-Heiner GORRIS; Petr SKLÁDAL and Zdeněk FARKA. Single-molecule microfluidic assay for prostate-specific antigen based on magnetic beads and upconversion nanoparticles. Lab on a Chip. Cambridge: Royal Society of Chemistry, 2024, vol. 24, No 14, p. 3536-3545. ISSN 1473-0197. Available from: https://dx.doi.org/10.1039/D4LC00346B.

@article{2417985,
   author = {Sklenárová, Dorota and Hlaváček, Antonín and Křivánková, Jana and Brandmeier, Julian and Weisová, Julie and Řiháček, Michal and Gorris, HansandHeiner and Skládal, Petr and Farka, Zdeněk},
   article_location = {Cambridge},
   article_number = {14},
   doi = {http://dx.doi.org/10.1039/D4LC00346B},
   keywords = {Photon-upconversion nanoparticle; Immunoassay; Microfluidics; Prostate-specific antigen},
   language = {eng},
   issn = {1473-0197},
   journal = {Lab on a Chip},
   title = {Single-molecule microfluidic assay for prostate-specific antigen based on magnetic beads and upconversion nanoparticles},
   url = {https://pubs.rsc.org/en/content/articlelanding/2024/lc/d4lc00346b},
   volume = {24},
   year = {2024}
}

TY  - JOUR
ID  - 2417985
AU  - Sklenárová, Dorota - Hlaváček, Antonín - Křivánková, Jana - Brandmeier, Julian - Weisová, Julie - Řiháček, Michal - Gorris, Hans-Heiner - Skládal, Petr - Farka, Zdeněk
PY  - 2024
TI  - Single-molecule microfluidic assay for prostate-specific antigen based on magnetic beads and upconversion nanoparticles
JF  - Lab on a Chip
VL  - 24
IS  - 14
SP  - 3536-3545
EP  - 3536-3545
PB  - Royal Society of Chemistry
SN  - 14730197
KW  - Photon-upconversion nanoparticle
KW  - Immunoassay
KW  - Microfluidics
KW  - Prostate-specific antigen
UR  - https://pubs.rsc.org/en/content/articlelanding/2024/lc/d4lc00346b
N2  - Early-stage diagnosis of prostatic carcinoma is essential for successful treatment and, thus, significant prognosis improvement. In laboratory practice, the standard non-invasive diagnostic approach is the immunochemical detection of the associated biomarker, prostate-specific antigen (PSA). Ultrasensitive detection of PSA is essential for both diagnostic and recurrence monitoring purposes. To achieve exceptional sensitivity, we have developed a microfluidic device with a flow-through cell for single-molecule analysis using photon-upconversion nanoparticles (UCNPs) as a detection label. For this purpose, magnetic microparticles (MBs) were first optimized for the capture and preconcentration of PSA and then used to implement a bead-based upconversion-linked immunoassay (ULISA) in the microfluidic device. The digital readout based on counting single nanoparticle-labeled PSA molecules on MBs enabled a detection limit of 1.04 pg mL−1 (36 fM) in 50% fetal bovine serum, which is an 11-fold improvement over the respective analog MB-based ULISA. The microfluidic technique conferred several other advantages, such as easy implementation and the potential for achieving high-throughput analysis. Finally, it was proven that the microfluidic setup is suitable for clinical sample analysis, showing a good correlation with a reference electrochemiluminescence assay (recovery rates between 97% and 105%).
ER  -

SKLENÁROVÁ, Dorota; Antonín HLAVÁČEK; Jana KŘIVÁNKOVÁ; Julian BRANDMEIER; Julie WEISOVÁ; Michal ŘIHÁČEK; Hans-Heiner GORRIS; Petr SKLÁDAL and Zdeněk FARKA. Single-molecule microfluidic assay for prostate-specific antigen based on magnetic beads and upconversion nanoparticles. \textit{Lab on a Chip}. Cambridge: Royal Society of Chemistry, 2024, vol.~24, No~14, p.~3536-3545. ISSN~1473-0197. Available from: https://dx.doi.org/10.1039/D4LC00346B.

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