J 2024

Verifying the cytotoxicity of a biodegradable zinc alloy with nanodiamond sensors

WOJTAS, Daniel, Aldona MZYK, Runrun LI, Michael ZEHETBAUER, Erhard SCHAFLER et. al.

Basic information

Original name

Verifying the cytotoxicity of a biodegradable zinc alloy with nanodiamond sensors

Authors

WOJTAS, Daniel (616 Poland, belonging to the institution), Aldona MZYK, Runrun LI, Michael ZEHETBAUER, Erhard SCHAFLER, Anna JARZEBSKA, Bartosz SULKOWSKI and Romana SCHIRHAGL

Edition

Biomaterials Advances, AMSTERDAM, Elsevier B.V. 2024, 2772-9508

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

21001 Nano-materials

Country of publisher

Netherlands

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.1016/j.bioadv.2024.213927

UT WoS

001260769500001

Keywords in English

Biodegradable zinc; Cytotoxicity; Free radical; Relaxometry; NV center; Nanodiamond

Tags

14110518, rivok

Tags

International impact, Reviewed
Změněno: 12/7/2024 08:30, Mgr. Tereza Miškechová

Abstract

V originále

Metals are widely utilized as implant materials for bone fixtures as well as stents. Biodegradable versions of these implants are highly desirable since patients do not have to undergo a second surgery for the materials to be removed. Attractive options for such materials are zinc silver alloys since they also offer the benefit of being antibacterial. However, it is important to investigate the effect of the degradation products of such alloys on the surrounding cells, taking into account silver cytotoxicity. Here we investigated zinc alloyed with 1 % of silver (Zn-1Ag) and how differently concentrated extracts (1 %-100 %) of this material impact human umbilical vein endothelial cells (HUVECs). More specifically, we focused on free radical generation and oxidative stress as well as the impact on cell viability. To determine free radical production we used diamond-based quantum sensing as well as conventional fluorescent assays. The viability was assessed by observing cell morphology and the metabolic activity via the MTT assay. We found that 1 % and 10 % extracts are well tolerated by the cells. However, at higher extract concentrations we observed severe impact on cell viability and oxidative stress. We were also able to show that quantum sensing was able to detect significant free radical generation even at the lowest tested concentrations.
Displayed: 5/11/2024 09:59