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@article{2418113,
author = {Vacek, Lukáš and Polaštík Kleknerová, Dominika and Lipový, Břetislav and Holoubek, Jakub and Matysková, Dominika and Cerna, E. and Brtnikova, J. and Jeklova, E. and Kobzova, S. and Janda, L. and Lišková, Lenka and Diabelko, Daniel and Botka, Tibor and Pantůček, Roman and Růžička, Filip and Vojtova, L.},
article_location = {AMSTERDAM},
article_number = {July 2024},
doi = {http://dx.doi.org/10.1016/j.ijpharm.2024.124348},
keywords = {Bacteriophage; Phage therapy; Gum Karaya; Hydrogel film; Injectable hydrogel; Staphylococcus aureus; MRSA},
language = {eng},
issn = {0378-5173},
journal = {International Journal of Pharmaceutics},
title = {Phage therapy combined with Gum Karaya injectable hydrogels for treatment of methicillin-resistant Staphylococcus aureus deep wound infection in a porcine model},
url = {https://www.sciencedirect.com/science/article/pii/S0378517324005829?via%3Dihub},
volume = {660},
year = {2024}
}
TY - JOUR
ID - 2418113
AU - Vacek, Lukáš - Polaštík Kleknerová, Dominika - Lipový, Břetislav - Holoubek, Jakub - Matysková, Dominika - Cerna, E. - Brtnikova, J. - Jeklova, E. - Kobzova, S. - Janda, L. - Lišková, Lenka - Diabelko, Daniel - Botka, Tibor - Pantůček, Roman - Růžička, Filip - Vojtova, L.
PY - 2024
TI - Phage therapy combined with Gum Karaya injectable hydrogels for treatment of methicillin-resistant Staphylococcus aureus deep wound infection in a porcine model
JF - International Journal of Pharmaceutics
VL - 660
IS - July 2024
SP - 1-11
EP - 1-11
PB - ELSEVIER
SN - 03785173
KW - Bacteriophage
KW - Phage therapy
KW - Gum Karaya
KW - Hydrogel film
KW - Injectable hydrogel
KW - Staphylococcus aureus
KW - MRSA
UR - https://www.sciencedirect.com/science/article/pii/S0378517324005829?via%3Dihub
N2 - Skin and soft tissue infections (SSTIs) represent a significant healthcare challenge, particularly in the context of increasing antibiotic resistance. This study investigates the efficacy of a novel therapeutic approach combining bacteriophage (phage) therapy with a gum Karaya (GK)-based hydrogel delivery system in a porcine model of deep staphylococcal SSTIs. The study exploits the lytic activity and safety of the Staphylococcus phage 812K1/ 420 of the Kayvirus genus, which is active against methicillin-resistant Staphylococcus aureus (MRSA). The GK injectable hydrogels and hydrogel films, developed by our research group, serve as effective, non-toxic, and easyto-apply delivery systems, supporting moist wound healing and re-epithelialization. In the porcine model, the combined treatment showed a synergistic effect, leading to a significant reduction in bacterial load (2.5 log CFU/ gram of tissue) within one week. Local signs of inflammation were significantly reduced by day 8, with clear evidence of re-epithelialization and wound contraction. Importantly, no adverse effects of the GK-based delivery system were observed throughout the study. The results highlight the potential of this innovative therapeutic approach to effectively treat deep staphylococcal SSTIs, providing a promising avenue for further research and clinical application in the field of infections caused by antibiotic-resistant bacteria.
ER -
VACEK, Lukáš, Dominika POLAŠTÍK KLEKNEROVÁ, Břetislav LIPOVÝ, Jakub HOLOUBEK, Dominika MATYSKOVÁ, E. CERNA, J. BRTNIKOVA, E. JEKLOVA, S. KOBZOVA, L. JANDA, Lenka LIŠKOVÁ, Daniel DIABELKO, Tibor BOTKA, Roman PANTŮČEK, Filip RŮŽIČKA and L. VOJTOVA. Phage therapy combined with Gum Karaya injectable hydrogels for treatment of methicillin-resistant Staphylococcus aureus deep wound infection in a porcine model. \textit{International Journal of Pharmaceutics}. AMSTERDAM: ELSEVIER, 2024, vol.~660, July 2024, p.~1-11. ISSN~0378-5173. Available from: https://dx.doi.org/10.1016/j.ijpharm.2024.124348.
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