Rapid determination of uracil in biological fluids at mercury
thin film electrode for early detection of potential
5-fluorouracil toxicity due to dihydropyrimidine dehydrogenase
deficiency

J 2024

Rapid determination of uracil in biological fluids at mercury thin film electrode for early detection of potential 5-fluorouracil toxicity due to dihydropyrimidine dehydrogenase deficiency

ASHRAFI, Amir M, Ozge SELCUK, Atripan MUKHERJEE, Didem Nur UNAL, Sevinc KURBANOGLU et. al.

Basic information

Original name

Rapid determination of uracil in biological fluids at mercury thin film electrode for early detection of potential 5-fluorouracil toxicity due to dihydropyrimidine dehydrogenase deficiency

Authors

ASHRAFI, Amir M, Ozge SELCUK, Atripan MUKHERJEE, Didem Nur UNAL, Sevinc KURBANOGLU, Bengi USLU, Jan JUŘICA (203 Czech Republic, belonging to the institution), Jana PEKARKOVA (203 Czech Republic), Lukas RICHTERA (203 Czech Republic) and Vojtech ADAM (203 Czech Republic)

Edition

Biosensors and Bioelectronics, OXFORD, ELSEVIER ADVANCED TECHNOLOGY, 2024, 0956-5663

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30104 Pharmacology and pharmacy

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 12.600 in 2022

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.1016/j.bios.2024.116545

UT WoS

001266783000001

Keywords in English

Mercury thin film electrode; Adsorptive stripping voltammetry; Uracil determination; Dihydropyrimidine dehydrogenase phenotyping; 5-Fluorouracil-based therapies

Abstract

V originále

Determination of plasma uracil was reported as a method for evaluation of Dihydropyrimidine dehydrogenase (DPD) activity that is highly demanded to ensure the safe administration of 5-fluorouracil (5-FU)-based therapies to cancer patients. This work reports the development of a simple electroanalytical method based on adsorptive stripping square wave voltammetry (AdSWV) at mercury film-coated glassy carbon electrode (MF/GCE) for the highly sensitive determination of uracil in biological fluids that can be used for diagnosis of decreased DPD activity. Due to the formation of the HgII–Uracil complex at the electrode surface, the accuracy of the measurement was not affected by the complicated matrices in biological fluids including human serum, plasma, and urine. The high sensitivity of the developed method results in a low limit of detection (≈1.3 nM) in human plasma samples, falling below the practical cut-off level of 15 ng mL−1 (≈0.14 μM). This threshold concentration is crucial for predicting 5-FU toxicity, as reported in buffer, and ≤1.15% in biological samples), and accuracy (recovery percentage close to 100%).

Links

90251, large research infrastructures

Name: CzechNanoLab II

Citovat

ASHRAFI, Amir M, Ozge SELCUK, Atripan MUKHERJEE, Didem Nur UNAL, Sevinc KURBANOGLU, Bengi USLU, Jan JUŘICA, Jana PEKARKOVA, Lukas RICHTERA and Vojtech ADAM. Rapid determination of uracil in biological fluids at mercury thin film electrode for early detection of potential 5-fluorouracil toxicity due to dihydropyrimidine dehydrogenase deficiency. Biosensors and Bioelectronics. OXFORD: ELSEVIER ADVANCED TECHNOLOGY, 2024, vol. 262, October 2024, p. 1-7. ISSN 0956-5663. Available from: https://dx.doi.org/10.1016/j.bios.2024.116545.

@article{2418611,
   author = {Ashrafi, Amir M and Selcuk, Ozge and Mukherjee, Atripan and Unal, Didem Nur and Kurbanoglu, Sevinc and Uslu, Bengi and Juřica, Jan and Pekarkova, Jana and Richtera, Lukas and Adam, Vojtech},
   article_location = {OXFORD},
   article_number = {October 2024},
   doi = {http://dx.doi.org/10.1016/j.bios.2024.116545},
   keywords = {Mercury thin film electrode; Adsorptive stripping voltammetry; Uracil determination; Dihydropyrimidine dehydrogenase phenotyping; 5-Fluorouracil-based therapies},
   language = {eng},
   issn = {0956-5663},
   journal = {Biosensors and Bioelectronics},
   title = {Rapid determination of uracil in biological fluids at mercury thin film electrode for early detection of potential 5-fluorouracil toxicity due to dihydropyrimidine dehydrogenase deficiency},
   url = {https://www.sciencedirect.com/science/article/pii/S0956566324005505},
   volume = {262},
   year = {2024}
}

TY  - JOUR
ID  - 2418611
AU  - Ashrafi, Amir M - Selcuk, Ozge - Mukherjee, Atripan - Unal, Didem Nur - Kurbanoglu, Sevinc - Uslu, Bengi - Juřica, Jan - Pekarkova, Jana - Richtera, Lukas - Adam, Vojtech
PY  - 2024
TI  - Rapid determination of uracil in biological fluids at mercury thin film electrode for early detection of potential 5-fluorouracil toxicity due to dihydropyrimidine dehydrogenase deficiency
JF  - Biosensors and Bioelectronics
VL  - 262
IS  - October 2024
SP  - 1-7
EP  - 1-7
PB  - ELSEVIER ADVANCED TECHNOLOGY
SN  - 09565663
KW  - Mercury thin film electrode
KW  - Adsorptive stripping voltammetry
KW  - Uracil determination
KW  - Dihydropyrimidine dehydrogenase phenotyping
KW  - 5-Fluorouracil-based therapies
UR  - https://www.sciencedirect.com/science/article/pii/S0956566324005505
N2  - Determination of plasma uracil was reported as a method for evaluation of Dihydropyrimidine dehydrogenase (DPD) activity that is highly demanded to ensure the safe administration of 5-fluorouracil (5-FU)-based therapies to cancer patients. This work reports the development of a simple electroanalytical method based on adsorptive stripping square wave voltammetry (AdSWV) at mercury film-coated glassy carbon electrode (MF/GCE) for the highly sensitive determination of uracil in biological fluids that can be used for diagnosis of decreased DPD activity. Due to the formation of the HgII–Uracil complex at the electrode surface, the accuracy of the measurement was not affected by the complicated matrices in biological fluids including human serum, plasma, and urine. The high sensitivity of the developed method results in a low limit of detection (≈1.3 nM) in human plasma samples, falling below the practical cut-off level of 15 ng mL−1 (≈0.14 μM). This threshold concentration is crucial for predicting 5-FU toxicity, as reported in buffer, and ≤1.15% in biological samples), and accuracy (recovery percentage close to 100%).
ER  -

ASHRAFI, Amir M, Ozge SELCUK, Atripan MUKHERJEE, Didem Nur UNAL, Sevinc KURBANOGLU, Bengi USLU, Jan JUŘICA, Jana PEKARKOVA, Lukas RICHTERA and Vojtech ADAM. Rapid determination of uracil in biological fluids at mercury thin film electrode for early detection of potential 5-fluorouracil toxicity due to dihydropyrimidine dehydrogenase deficiency. \textit{Biosensors and Bioelectronics}. OXFORD: ELSEVIER ADVANCED TECHNOLOGY, 2024, vol.~262, October 2024, p.~1-7. ISSN~0956-5663. Available from: https://dx.doi.org/10.1016/j.bios.2024.116545.

Detailed Information on Publication Record