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@article{2418700,
author = {Tomaštíková, Eva Dvořák and Vaculíková, Jitka and Štenclová, Veronika and Kaduchová, Kateřina and Pobořilová, Zuzana and Paleček, Jan and Pečinka, Aleš},
article_location = {ENGLAND},
doi = {http://dx.doi.org/10.1111},
language = {eng},
issn = {0960-7412},
journal = {PLANT JOURNAL},
title = {The interplay of homology-directed repair pathways in the repair of zebularine-induced DNA–protein crosslinks in Arabidopsis},
url = {https://onlinelibrary.wiley.com/doi/10.1111/tpj.16863},
year = {2024}
}
TY - JOUR
ID - 2418700
AU - Tomaštíková, Eva Dvořák - Vaculíková, Jitka - Štenclová, Veronika - Kaduchová, Kateřina - Pobořilová, Zuzana - Paleček, Jan - Pečinka, Aleš
PY - 2024
TI - The interplay of homology-directed repair pathways in the repair of zebularine-induced DNA–protein crosslinks in Arabidopsis
JF - PLANT JOURNAL
PB - WILEY
SN - 09607412
UR - https://onlinelibrary.wiley.com/doi/10.1111/tpj.16863
N2 - DNA–protein crosslinks (DPCs) are highly toxic DNA lesions represented by proteins covalently bound to the DNA. Persisting DPCs interfere with fundamental genetic processes such as DNA replication and transcription. Cytidine analog zebularine (ZEB) has been shown to crosslink DNA METHYLTRANSFERASE1 (MET1). Recently, we uncovered a critical role of the SMC5/6-mediated SUMOylation in the repair of DPCs. In an ongoing genetic screen, we identified two additional candidates, HYPERSENSITIVE TO ZEBULARINE 2 and 3, that were mapped to REGULATOR OF TELOMERE ELONGATION 1 (RTEL1) and polymerase TEBICHI (TEB), respectively. By monitoring the growth of hze2 and hze3 plants in response to zebularine, we show the importance of homologous recombination (HR) factor RTEL1 and microhomology-mediated end-joining (MMEJ) polymerase TEB in the repair of MET1-DPCs. Moreover, genetic interaction and sensitivity assays showed the interdependency of SMC5/6 complex, HR, and MMEJ in the homology-directed repair of MET1-DPCs in Arabidopsis. Altogether, we provide evidence that MET1-DPC repair in plants is more complex than originally expected.
ER -
TOMAŠTÍKOVÁ, Eva Dvořák, Jitka VACULÍKOVÁ, Veronika ŠTENCLOVÁ, Kateřina KADUCHOVÁ, Zuzana POBOŘILOVÁ, Jan PALEČEK and Aleš PEČINKA. The interplay of homology-directed repair pathways in the repair of zebularine-induced DNA–protein crosslinks in Arabidopsis. \textit{PLANT JOURNAL}. ENGLAND: WILEY, 2024. ISSN~0960-7412. Available from: https://dx.doi.org/10.1111.
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