Cytotoxic effects and comparative analysis of Ni ion uptake by
osteoarthritic and physiological osteoblasts

J 2024

Cytotoxic effects and comparative analysis of Ni ion uptake by osteoarthritic and physiological osteoblasts

NAVRÁTILOVÁ, Polina, Markéta VEJVODOVÁ, Tomáš VACULOVIČ, Iva SLANINOVÁ, Jan EMMER et. al.

Basic information

Original name

Cytotoxic effects and comparative analysis of Ni ion uptake by osteoarthritic and physiological osteoblasts

Authors

NAVRÁTILOVÁ, Polina (643 Russian Federation, belonging to the institution), Markéta VEJVODOVÁ (203 Czech Republic, belonging to the institution), Tomáš VACULOVIČ (203 Czech Republic, belonging to the institution), Iva SLANINOVÁ (203 Czech Republic, belonging to the institution), Jan EMMER (203 Czech Republic), Tomáš TOMÁŠ (203 Czech Republic, belonging to the institution), Luděk RYBA (203 Czech Republic), Jan BURDA (203 Czech Republic) and Monika PÁVKOVÁ GOLDBERGOVÁ (203 Czech Republic, belonging to the institution)

Edition

Scientific Reports, BERLIN, NATURE PORTFOLIO, 2024, 2045-2322

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10601 Cell biology

Country of publisher

Germany

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 4.600 in 2022

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.1038/s41598-024-67157-9

UT WoS

001270360800063

Keywords in English

Ni ion uptake; osteoarthritic osteoblasts; physiological osteoblasts

Abstract

V originále

Nickel(Ni)-containing materials have been widely used in a wide range of medical applications, including orthopaedics. Despite their excellent properties, there is still a problem with the release of nickel ions into the patient’s body, which can cause changes in the behaviour of surrounding cells and tissues. This study aims to evaluate the effects of Ni on bone cells with an emphasis on the determination of Ni localization in cellular compartments in time. For these purposes, one of the most suitable models for studying the effects induced by metal implants was used—the patient’s osteoarthritic cells. Thanks to this it was possible to simulate the pathophysiological conditions in the patient’s body, as well as to evaluate the response of the cells which come into direct contact with the material after the implantation of the joint replacement. The largest differences in cell viability, proliferation and cell cycle changes occurred between Ni 0.5 mM and 1 mM concentrations. Time-dependent localization of Ni in cells showed that there is a continuous transport of Ni ions between the nucleus and the cytoplasm, as well as between the cell and the environment. Moreover, osteoarthritic osteoblasts showed faster changes in concentration and ability to accumulate more Ni, especially in the nucleus, than physiological osteoblasts. The differences in Ni accumulation process explains the higher sensitivity of patient osteoblasts to Ni and may be crucial in further studies of implant-derived cytotoxic effects.

Links

MUNI/A/1575/2023, interní kód MU

Name: Metody chemické a fyzikálně-chemické analýzy pro studium přírodních a syntetických materiálů.

Investor: Masaryk University, Methods of chemical and physico-chemical analysis for the study of natural and synthetic materials.

NU20-08-00149, research and development project

Name: Multicentrické hodnocení hypersenzitivní reakce u pacientů indikovaných k totální náhradě kloubu včetně hodnocení důvodů reimplanace

Investor: Ministry of Health of the CR, Subprogram 1 - standard

Citovat

NAVRÁTILOVÁ, Polina, Markéta VEJVODOVÁ, Tomáš VACULOVIČ, Iva SLANINOVÁ, Jan EMMER, Tomáš TOMÁŠ, Luděk RYBA, Jan BURDA and Monika PÁVKOVÁ GOLDBERGOVÁ. Cytotoxic effects and comparative analysis of Ni ion uptake by osteoarthritic and physiological osteoblasts. Scientific Reports. BERLIN: NATURE PORTFOLIO, 2024, vol. 14, No 1, p. 1-15. ISSN 2045-2322. Available from: https://dx.doi.org/10.1038/s41598-024-67157-9.

@article{2420072,
   author = {Navrátilová, Polina and Vejvodová, Markéta and Vaculovič, Tomáš and Slaninová, Iva and Emmer, Jan and Tomáš, Tomáš and Ryba, Luděk and Burda, Jan and Pávková Goldbergová, Monika},
   article_location = {BERLIN},
   article_number = {1},
   doi = {http://dx.doi.org/10.1038/s41598-024-67157-9},
   keywords = {Ni ion uptake; osteoarthritic osteoblasts; physiological osteoblasts},
   language = {eng},
   issn = {2045-2322},
   journal = {Scientific Reports},
   title = {Cytotoxic effects and comparative analysis of Ni ion uptake by osteoarthritic and physiological osteoblasts},
   url = {https://www.nature.com/articles/s41598-024-67157-9},
   volume = {14},
   year = {2024}
}

TY  - JOUR
ID  - 2420072
AU  - Navrátilová, Polina - Vejvodová, Markéta - Vaculovič, Tomáš - Slaninová, Iva - Emmer, Jan - Tomáš, Tomáš - Ryba, Luděk - Burda, Jan - Pávková Goldbergová, Monika
PY  - 2024
TI  - Cytotoxic effects and comparative analysis of Ni ion uptake by osteoarthritic and physiological osteoblasts
JF  - Scientific Reports
VL  - 14
IS  - 1
SP  - 1-15
EP  - 1-15
PB  - NATURE PORTFOLIO
SN  - 20452322
KW  - Ni ion uptake
KW  - osteoarthritic osteoblasts
KW  - physiological osteoblasts
UR  - https://www.nature.com/articles/s41598-024-67157-9
N2  - Nickel(Ni)-containing materials have been widely used in a wide range of medical applications, including orthopaedics. Despite their excellent properties, there is still a problem with the release of nickel ions into the patient’s body, which can cause changes in the behaviour of surrounding cells and tissues. This study aims to evaluate the effects of Ni on bone cells with an emphasis on the determination of Ni localization in cellular compartments in time. For these purposes, one of the most suitable models for studying the effects induced by metal implants was used—the patient’s osteoarthritic cells. Thanks to this it was possible to simulate the pathophysiological conditions in the patient’s body, as well as to evaluate the response of the cells which come into direct contact with the material after the implantation of the joint replacement. The largest differences in cell viability, proliferation and cell cycle changes occurred between Ni 0.5 mM and 1 mM concentrations. Time-dependent localization of Ni in cells showed that there is a continuous transport of Ni ions between the nucleus and the cytoplasm, as well as between the cell and the environment. Moreover, osteoarthritic osteoblasts showed faster changes in concentration and ability to accumulate more Ni, especially in the nucleus, than physiological osteoblasts. The differences in Ni accumulation process explains the higher sensitivity of patient osteoblasts to Ni and may be crucial in further studies of implant-derived cytotoxic effects.
ER  -

NAVRÁTILOVÁ, Polina, Markéta VEJVODOVÁ, Tomáš VACULOVIČ, Iva SLANINOVÁ, Jan EMMER, Tomáš TOMÁŠ, Luděk RYBA, Jan BURDA and Monika PÁVKOVÁ GOLDBERGOVÁ. Cytotoxic effects and comparative analysis of Ni ion uptake by osteoarthritic and physiological osteoblasts. \textit{Scientific Reports}. BERLIN: NATURE PORTFOLIO, 2024, vol.~14, No~1, p.~1-15. ISSN~2045-2322. Available from: https://dx.doi.org/10.1038/s41598-024-67157-9.

Detailed Information on Publication Record