Long circulating liposomal platform utilizing hydrophilic
polymer-based surface modification: preparation,
characterisation, and biological evaluation

J 2024

Long circulating liposomal platform utilizing hydrophilic polymer-based surface modification: preparation, characterisation, and biological evaluation

TURANEK, Jaroslav, Petr KOSZTYU, Pavlina TURANEK KNOTIGOVA, Eliska BARTHELDYOVA, Frantisek HUBATKA et. al.

Basic information

Original name

Long circulating liposomal platform utilizing hydrophilic polymer-based surface modification: preparation, characterisation, and biological evaluation

Authors

TURANEK, Jaroslav (203 Czech Republic), Petr KOSZTYU (203 Czech Republic), Pavlina TURANEK KNOTIGOVA (203 Czech Republic), Eliska BARTHELDYOVA (203 Czech Republic), Frantisek HUBATKA (203 Czech Republic), Nikola ODEHNALOVA (203 Czech Republic), Robert MIKULÍK (203 Czech Republic), Naděžda VAŠKOVICOVÁ (203 Czech Republic, belonging to the institution), Hana ČELECHOVSKÁ (203 Czech Republic, belonging to the institution), Irena KRATOCHVILOVA (203 Czech Republic), Ladislav FEKETE (203 Czech Republic), Marina R. TAVARES (203 Czech Republic), Petr CHYTIL (203 Czech Republic), Milan RASKA (203 Czech Republic) and Tomas ETRYCH (203 Czech Republic)

Edition

International Journal of Pharmaceutics, AMSTERDAM, ELSEVIER, 2024, 0378-5173

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10608 Biochemistry and molecular biology

Country of publisher

Netherlands

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 5.800 in 2022

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.1016/j.ijpharm.2024.124465

UT WoS

001273994500001

Keywords in English

Stealth liposomes; HPMA copolymer; Complement activation; Drug delivery system; Long-circulating liposomes

Abstract

V originále

Liposomes are one of the most important drug delivery vectors, nowadays used in clinics. In general, polyethylene glycol (PEG) is used to ensure the stealth properties of the liposomes. Here, we have employed hydrophilic, biocompatible and highly non-fouling N-(2-hydroxypropyl) methacrylamide (HPMA)-based copolymers containing hydrophobic cholesterol anchors for the surface modification of liposomes, which were prepared by the method of lipid film hydration and extrusion through 100 nm polycarbonate filters. Efficient surface modification of liposomes was confirmed by transmission electron microscopy, atomic force microscopy, and gradient ultracentrifugation. The ability of long-term circulation in the vascular bed was demonstrated in rabbits after i.v. application of fluorescently labelled liposomes. Compared to PEGylated liposomes, HPMA-based copolymer-modified liposomes did not induce specific antibody formation and did not activate murine and human complement. Compared with PEGylated liposomes, HPMA-based copolymer-modified liposomes showed a better long-circulating effect after repeated administration. HPMA-based copolymer-modified liposomes thus represent suitable new candidates for a generation of safer and improved liposomal drug delivery platforms.

Links

EH22_008/0004596, research and development project

Name: Senzory a detektory pro informační společnost budoucnosti

NU21-08-00510, research and development project

Name: Biokompatibilní nanočástice jako cílené systémy pro dodávání léčiv a teranostik pro léčbu cévní mozkové příhody

Investor: Ministry of Health of the CR

Citovat

TURANEK, Jaroslav, Petr KOSZTYU, Pavlina TURANEK KNOTIGOVA, Eliska BARTHELDYOVA, Frantisek HUBATKA, Nikola ODEHNALOVA, Robert MIKULÍK, Naděžda VAŠKOVICOVÁ, Hana ČELECHOVSKÁ, Irena KRATOCHVILOVA, Ladislav FEKETE, Marina R. TAVARES, Petr CHYTIL, Milan RASKA and Tomas ETRYCH. Long circulating liposomal platform utilizing hydrophilic polymer-based surface modification: preparation, characterisation, and biological evaluation. International Journal of Pharmaceutics. AMSTERDAM: ELSEVIER, 2024, vol. 661, August 2024, p. 1-14. ISSN 0378-5173. Available from: https://dx.doi.org/10.1016/j.ijpharm.2024.124465.

@article{2421640,
   author = {Turanek, Jaroslav and Kosztyu, Petr and Turanek Knotigova, Pavlina and Bartheldyova, Eliska and Hubatka, Frantisek and Odehnalova, Nikola and Mikulík, Robert and Vaškovicová, Naděžda and Čelechovská, Hana and Kratochvilova, Irena and Fekete, Ladislav and Tavares, Marina R. and Chytil, Petr and Raska, Milan and Etrych, Tomas},
   article_location = {AMSTERDAM},
   article_number = {August 2024},
   doi = {http://dx.doi.org/10.1016/j.ijpharm.2024.124465},
   keywords = {Stealth liposomes; HPMA copolymer; Complement activation; Drug delivery system; Long-circulating liposomes},
   language = {eng},
   issn = {0378-5173},
   journal = {International Journal of Pharmaceutics},
   title = {Long circulating liposomal platform utilizing hydrophilic polymer-based surface modification: preparation, characterisation, and biological evaluation},
   url = {https://www.sciencedirect.com/science/article/pii/S0378517324006999?via%3Dihub},
   volume = {661},
   year = {2024}
}

TY  - JOUR
ID  - 2421640
AU  - Turanek, Jaroslav - Kosztyu, Petr - Turanek Knotigova, Pavlina - Bartheldyova, Eliska - Hubatka, Frantisek - Odehnalova, Nikola - Mikulík, Robert - Vaškovicová, Naděžda - Čelechovská, Hana - Kratochvilova, Irena - Fekete, Ladislav - Tavares, Marina R. - Chytil, Petr - Raska, Milan - Etrych, Tomas
PY  - 2024
TI  - Long circulating liposomal platform utilizing hydrophilic polymer-based surface modification: preparation, characterisation, and biological evaluation
JF  - International Journal of Pharmaceutics
VL  - 661
IS  - August 2024
SP  - 1-14
EP  - 1-14
PB  - ELSEVIER
SN  - 03785173
KW  - Stealth liposomes
KW  - HPMA copolymer
KW  - Complement activation
KW  - Drug delivery system
KW  - Long-circulating liposomes
UR  - https://www.sciencedirect.com/science/article/pii/S0378517324006999?via%3Dihub
N2  - Liposomes are one of the most important drug delivery vectors, nowadays used in clinics. In general, polyethylene glycol (PEG) is used to ensure the stealth properties of the liposomes. Here, we have employed hydrophilic, biocompatible and highly non-fouling N-(2-hydroxypropyl) methacrylamide (HPMA)-based copolymers containing hydrophobic cholesterol anchors for the surface modification of liposomes, which were prepared by the method of lipid film hydration and extrusion through 100 nm polycarbonate filters. Efficient surface modification of liposomes was confirmed by transmission electron microscopy, atomic force microscopy, and gradient ultracentrifugation. The ability of long-term circulation in the vascular bed was demonstrated in rabbits after i.v. application of fluorescently labelled liposomes. Compared to PEGylated liposomes, HPMA-based copolymer-modified liposomes did not induce specific antibody formation and did not activate murine and human complement. Compared with PEGylated liposomes, HPMA-based copolymer-modified liposomes showed a better long-circulating effect after repeated administration. HPMA-based copolymer-modified liposomes thus represent suitable new candidates for a generation of safer and improved liposomal drug delivery platforms.
ER  -

TURANEK, Jaroslav, Petr KOSZTYU, Pavlina TURANEK KNOTIGOVA, Eliska BARTHELDYOVA, Frantisek HUBATKA, Nikola ODEHNALOVA, Robert MIKULÍK, Naděžda VAŠKOVICOVÁ, Hana ČELECHOVSKÁ, Irena KRATOCHVILOVA, Ladislav FEKETE, Marina R. TAVARES, Petr CHYTIL, Milan RASKA and Tomas ETRYCH. Long circulating liposomal platform utilizing hydrophilic polymer-based surface modification: preparation, characterisation, and biological evaluation. \textit{International Journal of Pharmaceutics}. AMSTERDAM: ELSEVIER, 2024, vol.~661, August 2024, p.~1-14. ISSN~0378-5173. Available from: https://dx.doi.org/10.1016/j.ijpharm.2024.124465.

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