Porous silica-doped calcium phosphate scaffolds prepared via
in-situ foaming method

J 2024

Porous silica-doped calcium phosphate scaffolds prepared via in-situ foaming method

SISKA VIRAGOVA, Eliska, Lenka NOVOTNA, Zdenek CHLUP, Premysl STASTNY, Pavlina SARFY et. al.

Basic information

Original name

Porous silica-doped calcium phosphate scaffolds prepared via in-situ foaming method

Authors

SISKA VIRAGOVA, Eliska (203 Czech Republic), Lenka NOVOTNA (203 Czech Republic), Zdenek CHLUP (203 Czech Republic), Premysl STASTNY (203 Czech Republic), Pavlina SARFY (203 Czech Republic), Jaroslav CIHLAR (203 Czech Republic), Martin KUČÍREK (203 Czech Republic, belonging to the institution), Leoš BENÁK (203 Czech Republic, belonging to the institution), Libor STREIT (203 Czech Republic), Jan KOCANDA (203 Czech Republic, belonging to the institution), Jan SKLENSKÝ (203 Czech Republic, belonging to the institution), Milan FILIPOVIČ (203 Czech Republic, belonging to the institution), Martin REPKO (203 Czech Republic), Aleš HAMPL (203 Czech Republic, belonging to the institution), Irena KOUTNÁ (203 Czech Republic, belonging to the institution) and Klara CASTKOVA (203 Czech Republic)

Edition

Ceramics International, London, Elsevier, 2024, 0272-8842

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

20601 Medical engineering

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 5.200 in 2022

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.1016/j.ceramint.2024.07.433

UT WoS

001327513100001

Keywords in English

Calcium phosphates; Silica; In-situ foaming; Mechanical strength; In vitro response

Abstract

V originále

The effect of silica (SiO2) addition (0 wt%-20 wt%) on the microstructural and mechanical properties, as well as the in vitro response of calcium phosphate scaffolds for potential application in bone tissue engineering (BTE) was investigated in this research. Scaffolds characterized by high porosity (77%–88 %) and interconnected spherical pores with a broad range of pore sizes (5–600 μm) were fabricated using in-situ foaming method. Incorporated silica affected the phase transformation of hydroxyapatite (HA) to β-tricalcium phosphate (β-TCP) and led to the development of new crystalline silica-rich phases like silicocarnotite and wollastonite. The reinforcement of silica became apparent during the tests of mechanical properties. Scaffolds with 5 wt% of SiO2 exhibited compressive strength (1.13 MPa) higher than pure HA scaffolds (0.93 MPa). Bone bonding potential of the materials was tested in simulated body fluid (SBF), demonstrating this potential in silica-doped samples. Additionally, degradation experiments showed gradual material degradation, making it suitable for BTE applications. Furthermore, cell culture studies using human mesenchymal stromal cells (MSC) confirmed the scaffold's non-toxicity and provided insights into how the silica content influences cell viability, morphology, and osteogenic potential. The findings of this study offer valuable insights into the design and development of advanced scaffolds with tailored properties for effective BTE applications.

Links

LM2023050, research and development project

Name: Národní infrastruktura pro biologické a medicínské zobrazování

Investor: Ministry of Education, Youth and Sports of the CR, Czech BioImaging: National research infrastructure for biological and medical imaging

MUNI/A/1598/2023, interní kód MU

Name: Zdroje pro tkáňové inženýrství 14

Investor: Masaryk University, Resources pro tissue engineering 14

NU20-08-00402, research and development project

Name: Bioarteficiální 3D štep pro meziobratlovou fúzi páteře

Investor: Ministry of Health of the CR, Subprogram 1 - standard

90251, large research infrastructures

Name: CzechNanoLab II

Citovat

SISKA VIRAGOVA, Eliska, Lenka NOVOTNA, Zdenek CHLUP, Premysl STASTNY, Pavlina SARFY, Jaroslav CIHLAR, Martin KUČÍREK, Leoš BENÁK, Libor STREIT, Jan KOCANDA, Jan SKLENSKÝ, Milan FILIPOVIČ, Martin REPKO, Aleš HAMPL, Irena KOUTNÁ and Klara CASTKOVA. Porous silica-doped calcium phosphate scaffolds prepared via in-situ foaming method. Ceramics International. London: Elsevier, 2024, vol. 50, No 21, p. 41215-41227. ISSN 0272-8842. Available from: https://dx.doi.org/10.1016/j.ceramint.2024.07.433.

@article{2421647,
   author = {Siska Viragova, Eliska and Novotna, Lenka and Chlup, Zdenek and Stastny, Premysl and Sarfy, Pavlina and Cihlar, Jaroslav and Kučírek, Martin and Benák, Leoš and Streit, Libor and Kocanda, Jan and Sklenský, Jan and Filipovič, Milan and Repko, Martin and Hampl, Aleš and Koutná, Irena and Castkova, Klara},
   article_location = {London},
   article_number = {21},
   doi = {http://dx.doi.org/10.1016/j.ceramint.2024.07.433},
   keywords = {Calcium phosphates; Silica; In-situ foaming; Mechanical strength; In vitro response},
   language = {eng},
   issn = {0272-8842},
   journal = {Ceramics International},
   title = {Porous silica-doped calcium phosphate scaffolds prepared via in-situ foaming method},
   url = {https://www.sciencedirect.com/science/article/pii/S0272884224033790},
   volume = {50},
   year = {2024}
}

TY  - JOUR
ID  - 2421647
AU  - Siska Viragova, Eliska - Novotna, Lenka - Chlup, Zdenek - Stastny, Premysl - Sarfy, Pavlina - Cihlar, Jaroslav - Kučírek, Martin - Benák, Leoš - Streit, Libor - Kocanda, Jan - Sklenský, Jan - Filipovič, Milan - Repko, Martin - Hampl, Aleš - Koutná, Irena - Castkova, Klara
PY  - 2024
TI  - Porous silica-doped calcium phosphate scaffolds prepared via in-situ foaming method
JF  - Ceramics International
VL  - 50
IS  - 21
SP  - 41215-41227
EP  - 41215-41227
PB  - Elsevier
SN  - 02728842
KW  - Calcium phosphates
KW  - Silica
KW  - In-situ foaming
KW  - Mechanical strength
KW  - In vitro response
UR  - https://www.sciencedirect.com/science/article/pii/S0272884224033790
N2  - The effect of silica (SiO2) addition (0 wt%-20 wt%) on the microstructural and mechanical properties, as well as the in vitro response of calcium phosphate scaffolds for potential application in bone tissue engineering (BTE) was investigated in this research. Scaffolds characterized by high porosity (77%–88 %) and interconnected spherical pores with a broad range of pore sizes (5–600 μm) were fabricated using in-situ foaming method. Incorporated silica affected the phase transformation of hydroxyapatite (HA) to β-tricalcium phosphate (β-TCP) and led to the development of new crystalline silica-rich phases like silicocarnotite and wollastonite. The reinforcement of silica became apparent during the tests of mechanical properties. Scaffolds with 5 wt% of SiO2 exhibited compressive strength (1.13 MPa) higher than pure HA scaffolds (0.93 MPa). Bone bonding potential of the materials was tested in simulated body fluid (SBF), demonstrating this potential in silica-doped samples. Additionally, degradation experiments showed gradual material degradation, making it suitable for BTE applications. Furthermore, cell culture studies using human mesenchymal stromal cells (MSC) confirmed the scaffold's non-toxicity and provided insights into how the silica content influences cell viability, morphology, and osteogenic potential. The findings of this study offer valuable insights into the design and development of advanced scaffolds with tailored properties for effective BTE applications.
ER  -

SISKA VIRAGOVA, Eliska, Lenka NOVOTNA, Zdenek CHLUP, Premysl STASTNY, Pavlina SARFY, Jaroslav CIHLAR, Martin KUČÍREK, Leoš BENÁK, Libor STREIT, Jan KOCANDA, Jan SKLENSKÝ, Milan FILIPOVIČ, Martin REPKO, Aleš HAMPL, Irena KOUTNÁ and Klara CASTKOVA. Porous silica-doped calcium phosphate scaffolds prepared via in-situ foaming method. \textit{Ceramics International}. London: Elsevier, 2024, vol.~50, No~21, p.~41215-41227. ISSN~0272-8842. Available from: https://dx.doi.org/10.1016/j.ceramint.2024.07.433.

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