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@article{2422976,
author = {Holomková, Kateřina and Vesela, Barbora and Dadáková, Kateřina and Sharpe, Paul T and Lesot, Herve and Matalova, Eva and Švandová, Eva},
article_location = {Heidelberg},
article_number = {9},
doi = {http://dx.doi.org/10.1007/s00424-024-03003-1},
keywords = {Dental pulp; Hypoxia-inducible factors; Development; In vivo},
language = {eng},
issn = {0031-6768},
journal = {Pflügers Archiv - European Journal of Physiology},
title = {Hypoxia-inducible factors in postnatal mouse molar dental pulp development: insights into expression patterns, localisation and metabolic pathways},
url = {https://link.springer.com/article/10.1007/s00424-024-03003-1},
volume = {476},
year = {2024}
}
TY - JOUR
ID - 2422976
AU - Holomková, Kateřina - Vesela, Barbora - Dadáková, Kateřina - Sharpe, Paul T - Lesot, Herve - Matalova, Eva - Švandová, Eva
PY - 2024
TI - Hypoxia-inducible factors in postnatal mouse molar dental pulp development: insights into expression patterns, localisation and metabolic pathways
JF - Pflügers Archiv - European Journal of Physiology
VL - 476
IS - 9
SP - 1411-1421
EP - 1411-1421
PB - Springer
SN - 00316768
KW - Dental pulp
KW - Hypoxia-inducible factors
KW - Development
KW - In vivo
UR - https://link.springer.com/article/10.1007/s00424-024-03003-1
N2 - Hypoxia is relevant to several physiological and pathological processes and this also applies for the tooth. The adaptive response to lowering oxygen concentration is mediated by hypoxia-inducible factors (HIFs). Since HIFs were shown to participate in the promotion of angiogenesis, stem cell survival, odontoblast differentiation and dentin formation, they may play a beneficial role in the tooth reparative processes. Although some data were generated in vitro, little is known about the in vivo context of HIFs in tooth development. In order to contribute to this field, the mouse mandibular first molar was used as a model.The expression and in situ localisation of HIFs were examined at postnatal (P) days P0, P7, P14, using RT-PCR and immunostaining. The expression pattern of a broad spectrum of hypoxia-related genes was monitored by customised PCR Arrays. Metabolic aspects were evaluated by determination of the lactate level and mRNA expression of the mitochondrial marker Nd1.The results show constant high mRNA expression of Hif1a, increasing expression of Hif2a, and very low expression of Hif3a during early postnatal molar development. In the examined period the localisation of HIFs in the nuclei of odontoblasts and the subodontoblastic layer identified their presence during odontoblastic differentiation. Additionally, the lower lactate level and higher expression of mitochondrial Nd1 in advanced development points to decreasing glycolysis during differentiation. Postnatal nuclear localisation of HIFs indicates a hypoxic state in specific areas of dental pulp as oxygen demands depend on physiological events such as crown and root dentin mineralization.
ER -
HOLOMKOVÁ, Kateřina, Barbora VESELA, Kateřina DADÁKOVÁ, Paul T SHARPE, Herve LESOT, Eva MATALOVA and Eva ŠVANDOVÁ. Hypoxia-inducible factors in postnatal mouse molar dental pulp development: insights into expression patterns, localisation and metabolic pathways. \textit{Pflügers Archiv - European Journal of Physiology}. Heidelberg: Springer, 2024, vol.~476, No~9, p.~1411-1421. ISSN~0031-6768. Available from: https://dx.doi.org/10.1007/s00424-024-03003-1.
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