Detailed Information on Publication Record
2024
Hypoxia-inducible factors in postnatal mouse molar dental pulp development: insights into expression patterns, localisation and metabolic pathways
HOLOMKOVÁ, Kateřina, Barbora VESELA, Kateřina DADÁKOVÁ, Paul T SHARPE, Herve LESOT et. al.Basic information
Original name
Hypoxia-inducible factors in postnatal mouse molar dental pulp development: insights into expression patterns, localisation and metabolic pathways
Authors
HOLOMKOVÁ, Kateřina (203 Czech Republic, belonging to the institution), Barbora VESELA (203 Czech Republic), Kateřina DADÁKOVÁ (203 Czech Republic, belonging to the institution), Paul T SHARPE, Herve LESOT, Eva MATALOVA (203 Czech Republic) and Eva ŠVANDOVÁ (203 Czech Republic, belonging to the institution)
Edition
Pflügers Archiv - European Journal of Physiology, Heidelberg, Springer, 2024, 0031-6768
Other information
Language
English
Type of outcome
Článek v odborném periodiku
Field of Study
30105 Physiology
Country of publisher
Germany
Confidentiality degree
není předmětem státního či obchodního tajemství
References:
Impact factor
Impact factor: 4.500 in 2022
Organization unit
Faculty of Medicine
UT WoS
001284675500002
Keywords in English
Dental pulp; Hypoxia-inducible factors; Development; In vivo
Tags
Změněno: 14/8/2024 10:59, Mgr. Tereza Miškechová
Abstract
V originále
Hypoxia is relevant to several physiological and pathological processes and this also applies for the tooth. The adaptive response to lowering oxygen concentration is mediated by hypoxia-inducible factors (HIFs). Since HIFs were shown to participate in the promotion of angiogenesis, stem cell survival, odontoblast differentiation and dentin formation, they may play a beneficial role in the tooth reparative processes. Although some data were generated in vitro, little is known about the in vivo context of HIFs in tooth development. In order to contribute to this field, the mouse mandibular first molar was used as a model.The expression and in situ localisation of HIFs were examined at postnatal (P) days P0, P7, P14, using RT-PCR and immunostaining. The expression pattern of a broad spectrum of hypoxia-related genes was monitored by customised PCR Arrays. Metabolic aspects were evaluated by determination of the lactate level and mRNA expression of the mitochondrial marker Nd1.The results show constant high mRNA expression of Hif1a, increasing expression of Hif2a, and very low expression of Hif3a during early postnatal molar development. In the examined period the localisation of HIFs in the nuclei of odontoblasts and the subodontoblastic layer identified their presence during odontoblastic differentiation. Additionally, the lower lactate level and higher expression of mitochondrial Nd1 in advanced development points to decreasing glycolysis during differentiation. Postnatal nuclear localisation of HIFs indicates a hypoxic state in specific areas of dental pulp as oxygen demands depend on physiological events such as crown and root dentin mineralization.
Links
| GA21-21409S, research and development project |
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