Zygotic spindle orientation defines cleavage pattern and
nuclear status of human embryos

J 2024

Zygotic spindle orientation defines cleavage pattern and nuclear status of human embryos

POROKH, Volodymyr, Drahomira KYJOVSKA, Martina MARTONOVA, Tereza KLENKOVA, Pavel OTEVREL et. al.

Basic information

Original name

Zygotic spindle orientation defines cleavage pattern and nuclear status of human embryos

Authors

POROKH, Volodymyr (804 Ukraine, belonging to the institution), Drahomira KYJOVSKA (203 Czech Republic), Martina MARTONOVA (203 Czech Republic), Tereza KLENKOVA (203 Czech Republic), Pavel OTEVREL (203 Czech Republic), Soňa KLOUDOVÁ (203 Czech Republic, belonging to the institution) and Zuzana HOLUBCOVÁ (203 Czech Republic, belonging to the institution)

Edition

Nature Communications, Berlin, Nature, 2024, 2041-1723

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10605 Developmental biology

Country of publisher

Germany

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 16.600 in 2022

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.1038/s41467-024-50732-z

UT WoS

001280592000005

Keywords in English

zygotic spindle orientation; human embryos; cleavage pattern; nuclear status

Abstract

V originále

The first embryonic division represents a starting point for the development of a new individual. In many species, tight control over the first embryonic division ensures its accuracy. However, the first division in humans is often erroneous and can impair embryo development. To delineate the spatiotemporal organization of the first mitotic division typical for normal human embryo development, we systematically analyzed a unique timelapse dataset of 300 IVF embryos that developed into healthy newborns. The zygotic division pattern of these best-quality embryos was compared to their siblings that failed to implant or arrested during cleavage stage. We show that division at the right angle to the juxtaposed pronuclei is preferential and supports faithful zygotic division. Alternative configurations of the first mitosis are associated with reduced clustering of nucleoli and multinucleation at the 2-cell stage, which are more common in women of advanced age. Collectively, these data imply that orientation of the first division predisposes human embryos to genetic (in)stability and may contribute to aneuploidy and age-related infertility.

Links

MUNI/A/1301/2022, interní kód MU

Name: Zdroje pro tkáňové inženýrství 13

Investor: Masaryk University

MUNI/A/1598/2023, interní kód MU

Name: Zdroje pro tkáňové inženýrství 14

Investor: Masaryk University, Resources pro tissue engineering 14

MUNI/IGA/1127/2021, interní kód MU

Name: Molecular organization of the zygotic spindle apparatus in non-rodent embryos

Investor: Masaryk University

Citovat

POROKH, Volodymyr, Drahomira KYJOVSKA, Martina MARTONOVA, Tereza KLENKOVA, Pavel OTEVREL, Soňa KLOUDOVÁ and Zuzana HOLUBCOVÁ. Zygotic spindle orientation defines cleavage pattern and nuclear status of human embryos. Nature Communications. Berlin: Nature, 2024, vol. 15, No 1, p. 1-9. ISSN 2041-1723. Available from: https://dx.doi.org/10.1038/s41467-024-50732-z.

@article{2423145,
   author = {Porokh, Volodymyr and Kyjovska, Drahomira and Martonova, Martina and Klenkova, Tereza and Otevrel, Pavel and Kloudová, Soňa and Holubcová, Zuzana},
   article_location = {Berlin},
   article_number = {1},
   doi = {http://dx.doi.org/10.1038/s41467-024-50732-z},
   keywords = {zygotic spindle orientation; human embryos; cleavage pattern; nuclear status},
   language = {eng},
   issn = {2041-1723},
   journal = {Nature Communications},
   title = {Zygotic spindle orientation defines cleavage pattern and nuclear status of human embryos},
   url = {https://www.nature.com/articles/s41467-024-50732-z},
   volume = {15},
   year = {2024}
}

TY  - JOUR
ID  - 2423145
AU  - Porokh, Volodymyr - Kyjovska, Drahomira - Martonova, Martina - Klenkova, Tereza - Otevrel, Pavel - Kloudová, Soňa - Holubcová, Zuzana
PY  - 2024
TI  - Zygotic spindle orientation defines cleavage pattern and nuclear status of human embryos
JF  - Nature Communications
VL  - 15
IS  - 1
SP  - 1-9
EP  - 1-9
PB  - Nature
SN  - 20411723
KW  - zygotic spindle orientation
KW  - human embryos
KW  - cleavage pattern
KW  - nuclear status
UR  - https://www.nature.com/articles/s41467-024-50732-z
N2  - The first embryonic division represents a starting point for the development of a new individual. In many species, tight control over the first embryonic division ensures its accuracy. However, the first division in humans is often erroneous and can impair embryo development. To delineate the spatiotemporal organization of the first mitotic division typical for normal human embryo development, we systematically analyzed a unique timelapse dataset of 300 IVF embryos that developed into healthy newborns. The zygotic division pattern of these best-quality embryos was compared to their siblings that failed to implant or arrested during cleavage stage. We show that division at the right angle to the juxtaposed pronuclei is preferential and supports faithful zygotic division. Alternative configurations of the first mitosis are associated with reduced clustering of nucleoli and multinucleation at the 2-cell stage, which are more common in women of advanced age. Collectively, these data imply that orientation of the first division predisposes human embryos to genetic (in)stability and may contribute to aneuploidy and age-related infertility.
ER  -

POROKH, Volodymyr, Drahomira KYJOVSKA, Martina MARTONOVA, Tereza KLENKOVA, Pavel OTEVREL, Soňa KLOUDOVÁ and Zuzana HOLUBCOVÁ. Zygotic spindle orientation defines cleavage pattern and nuclear status of human embryos. \textit{Nature Communications}. Berlin: Nature, 2024, vol.~15, No~1, p.~1-9. ISSN~2041-1723. Available from: https://dx.doi.org/10.1038/s41467-024-50732-z.

Detailed Information on Publication Record