Insight into antibacterial effect of titanium nanotubular
surfaces with focus on Staphylococcus aureus and Pseudomonas
aeruginosa

J 2024

Insight into antibacterial effect of titanium nanotubular surfaces with focus on Staphylococcus aureus and Pseudomonas aeruginosa

ŠÍSTKOVÁ, Jana, Tatiana FIALOVA, Emil SVOBODA, Kateřina VRCHOVECKÁ, Martin UHER et. al.

Basic information

Original name

Insight into antibacterial effect of titanium nanotubular surfaces with focus on Staphylococcus aureus and Pseudomonas aeruginosa

Authors

ŠÍSTKOVÁ, Jana (203 Czech Republic, belonging to the institution), Tatiana FIALOVA (203 Czech Republic), Emil SVOBODA (203 Czech Republic), Kateřina VRCHOVECKÁ (203 Czech Republic, belonging to the institution), Martin UHER (203 Czech Republic, belonging to the institution), Kristyna CIHALOVA (203 Czech Republic), Jan PŘIBYL (203 Czech Republic, belonging to the institution), Antonin DLOUHY (203 Czech Republic) and Monika PÁVKOVÁ GOLDBERGOVÁ (203 Czech Republic, belonging to the institution)

Edition

Scientific Reports, Berlin, NATURE RESEARCH, 2024, 2045-2322

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10601 Cell biology

Country of publisher

Germany

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 4.600 in 2022

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.1038/s41598-024-68266-1

UT WoS

001281273100069

Keywords in English

Titanium; Nanotubes; Anodization; Diameter; Roughness; Ti ion release; Bacterial behavior

Abstract

V originále

Materials used for orthopedic implants should not only have physical properties close to those of bones, durability and biocompatibility, but should also exhibit a sufficient degree of antibacterial functionality. Due to its excellent properties, titanium is still a widely used material for production of orthopedic implants, but the unmodified material exhibits poor antibacterial activity. In this work, the physicochemical characteristics, such as chemical composition, crystallinity, wettability, roughness, and release of Ti ions of the titanium surface modified with nanotubular layers were analyzed and its antibacterial activity against two biofilm-forming bacterial strains responsible for prosthetic joint infection (Staphylococcus aureus and Pseudomonas aeruginosa) was investigated. Electrochemical anodization (anodic oxidation) was used to prepare two types of nanotubular arrays with nanotubes differing in dimensions (with diameters of 73 and 118 nm and lengths of 572 and 343 nm, respectively). These two surface types showed similar chemistry, crystallinity, and surface energy. The surface with smaller nanotube diameter (TNT-73) but larger values of roughness parameters was more effective against S. aureus. For P. aeruginosa the sample with a larger nanotube diameter (TNT-118) had better antibacterial effect with proven cell lysis. Antibacterial properties of titanium nanotubular surfaces with potential in implantology, which in our previous work demonstrated a positive effect on the behavior of human gingival fibroblasts, were investigated in terms of surface parameters. The interplay between nanotube diameter and roughness appeared critical for the bacterial fate on nanotubular surfaces. The relationship of nanotube diameter, values of roughness parameters, and other surface properties to bacterial behavior is discussed in detail. The study is believed to shed more light on how nanotubular surface parameters and their interplay affect antibacterial activity.

Links

EF17_043/0009632, research and development project

Name: CETOCOEN Excellence

EF18_046/0015974, research and development project

Name: Modernizace České infrastruktury pro integrativní strukturní biologii

GA20-11321S, research and development project

Name: Vliv mikrostruktury a povrchových úprav na absorpci vodíku v bio-kompatibilních slitinách

Investor: Czech Science Foundation

LM2018121, research and development project

Name: Výzkumná infrastruktura RECETOX (Acronym: RECETOX RI)

Investor: Ministry of Education, Youth and Sports of the CR, RECETOX RI

LM2023042, research and development project

Name: Česká infrastruktura pro integrativní strukturní biologii

Investor: Ministry of Education, Youth and Sports of the CR, CIISB - Czech Infrastructure for Integrative Structural Biology

MUNI/A/1370/2022, interní kód MU

Name: Patofyziologie vybraných komplexních nemocí od molekulární do systémovou úroveň

Investor: Masaryk University, Pathophysiology of selected complex diseases from molecular to systemic level

Citovat

ŠÍSTKOVÁ, Jana, Tatiana FIALOVA, Emil SVOBODA, Kateřina VRCHOVECKÁ, Martin UHER, Kristyna CIHALOVA, Jan PŘIBYL, Antonin DLOUHY and Monika PÁVKOVÁ GOLDBERGOVÁ. Insight into antibacterial effect of titanium nanotubular surfaces with focus on Staphylococcus aureus and Pseudomonas aeruginosa. Scientific Reports. Berlin: NATURE RESEARCH, 2024, vol. 14, No 1, p. 1-20. ISSN 2045-2322. Available from: https://dx.doi.org/10.1038/s41598-024-68266-1.

@article{2423147,
   author = {Šístková, Jana and Fialova, Tatiana and Svoboda, Emil and Vrchovecká, Kateřina and Uher, Martin and Cihalova, Kristyna and Přibyl, Jan and Dlouhy, Antonin and Pávková Goldbergová, Monika},
   article_location = {Berlin},
   article_number = {1},
   doi = {http://dx.doi.org/10.1038/s41598-024-68266-1},
   keywords = {Titanium; Nanotubes; Anodization; Diameter; Roughness; Ti ion release; Bacterial behavior},
   language = {eng},
   issn = {2045-2322},
   journal = {Scientific Reports},
   title = {Insight into antibacterial effect of titanium nanotubular surfaces with focus on Staphylococcus aureus and Pseudomonas aeruginosa},
   url = {https://www.nature.com/articles/s41598-024-68266-1},
   volume = {14},
   year = {2024}
}

TY  - JOUR
ID  - 2423147
AU  - Šístková, Jana - Fialova, Tatiana - Svoboda, Emil - Vrchovecká, Kateřina - Uher, Martin - Cihalova, Kristyna - Přibyl, Jan - Dlouhy, Antonin - Pávková Goldbergová, Monika
PY  - 2024
TI  - Insight into antibacterial effect of titanium nanotubular surfaces with focus on Staphylococcus aureus and Pseudomonas aeruginosa
JF  - Scientific Reports
VL  - 14
IS  - 1
SP  - 1-20
EP  - 1-20
PB  - NATURE RESEARCH
SN  - 20452322
KW  - Titanium
KW  - Nanotubes
KW  - Anodization
KW  - Diameter
KW  - Roughness
KW  - Ti ion release
KW  - Bacterial behavior
UR  - https://www.nature.com/articles/s41598-024-68266-1
N2  - Materials used for orthopedic implants should not only have physical properties close to those of bones, durability and biocompatibility, but should also exhibit a sufficient degree of antibacterial functionality. Due to its excellent properties, titanium is still a widely used material for production of orthopedic implants, but the unmodified material exhibits poor antibacterial activity. In this work, the physicochemical characteristics, such as chemical composition, crystallinity, wettability, roughness, and release of Ti ions of the titanium surface modified with nanotubular layers were analyzed and its antibacterial activity against two biofilm-forming bacterial strains responsible for prosthetic joint infection (Staphylococcus aureus and Pseudomonas aeruginosa) was investigated. Electrochemical anodization (anodic oxidation) was used to prepare two types of nanotubular arrays with nanotubes differing in dimensions (with diameters of 73 and 118 nm and lengths of 572 and 343 nm, respectively). These two surface types showed similar chemistry, crystallinity, and surface energy. The surface with smaller nanotube diameter (TNT-73) but larger values of roughness parameters was more effective against S. aureus. For P. aeruginosa the sample with a larger nanotube diameter (TNT-118) had better antibacterial effect with proven cell lysis. Antibacterial properties of titanium nanotubular surfaces with potential in implantology, which in our previous work demonstrated a positive effect on the behavior of human gingival fibroblasts, were investigated in terms of surface parameters. The interplay between nanotube diameter and roughness appeared critical for the bacterial fate on nanotubular surfaces. The relationship of nanotube diameter, values of roughness parameters, and other surface properties to bacterial behavior is discussed in detail. The study is believed to shed more light on how nanotubular surface parameters and their interplay affect antibacterial activity.
ER  -

ŠÍSTKOVÁ, Jana, Tatiana FIALOVA, Emil SVOBODA, Kateřina VRCHOVECKÁ, Martin UHER, Kristyna CIHALOVA, Jan PŘIBYL, Antonin DLOUHY and Monika PÁVKOVÁ GOLDBERGOVÁ. Insight into antibacterial effect of titanium nanotubular surfaces with focus on Staphylococcus aureus and Pseudomonas aeruginosa. \textit{Scientific Reports}. Berlin: NATURE RESEARCH, 2024, vol.~14, No~1, p.~1-20. ISSN~2045-2322. Available from: https://dx.doi.org/10.1038/s41598-024-68266-1.

Detailed Information on Publication Record