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@article{2423158, author = {Jureckova, Katerina and Nykrynova, Marketa and Slaninova, Eva and FleuriotandBlitman, Hugo and Amstutz, Veronique and Hermankova, Kristyna and Bezdíček, Matěj and Mrazova, Katerina and Hrubanova, Kamila and Zinn, Manfred and Obruca, Stanislav and Sedlar, Karel}, article_location = {AMSTERDAM}, article_number = {December 2024}, doi = {http://dx.doi.org/10.1016/j.csbj.2024.06.023}, keywords = {Rhodospirillum rubrum; RNA-Seq; Transcriptome; Genome; Depolymerase knock-out; Gene ontology; Metabolism; Fructose; Acetate; Polyhydroxyalkanoates}, language = {eng}, issn = {2001-0370}, journal = {Computational and Structural Biotechnology Journal}, title = {Cultivation driven transcriptomic changes in the wild-type and mutant strains of Rhodospirillum rubrum}, url = {https://www.sciencedirect.com/science/article/pii/S2001037024002204?via%3Dihub}, volume = {23}, year = {2024} }
TY - JOUR ID - 2423158 AU - Jureckova, Katerina - Nykrynova, Marketa - Slaninova, Eva - Fleuriot-Blitman, Hugo - Amstutz, Veronique - Hermankova, Kristyna - Bezdíček, Matěj - Mrazova, Katerina - Hrubanova, Kamila - Zinn, Manfred - Obruca, Stanislav - Sedlar, Karel PY - 2024 TI - Cultivation driven transcriptomic changes in the wild-type and mutant strains of Rhodospirillum rubrum JF - Computational and Structural Biotechnology Journal VL - 23 IS - December 2024 SP - 2681-2694 EP - 2681-2694 PB - Elsevier SN - 20010370 KW - Rhodospirillum rubrum KW - RNA-Seq KW - Transcriptome KW - Genome KW - Depolymerase knock-out KW - Gene ontology KW - Metabolism KW - Fructose KW - Acetate KW - Polyhydroxyalkanoates UR - https://www.sciencedirect.com/science/article/pii/S2001037024002204?via%3Dihub N2 - Purple photosynthetic bacteria (PPB) are versatile microorganisms capable of producing various value-added chemicals, e.g., biopolymers and biofuels. They employ diverse metabolic pathways, allowing them to adapt to various growth conditions and even extreme environments. Thus, they are ideal organisms for the Next Generation Industrial Biotechnology concept of reducing the risk of contamination by using naturally robust extremophiles. Unfortunately, the potential of PPB for use in biotechnology is hampered by missing knowledge on regulations of their metabolism. Although Rhodospirillum rubrum represents a model purple bacterium studied for polyhydroxyalkanoate and hydrogen production, light/chemical energy conversion, and nitrogen fixation, little is known regarding the regulation of its metabolism at the transcriptomic level. Using RNA sequencing, we compared gene expression during the cultivation utilizing fructose and acetate as substrates in case of the wildtype strain R. rubrum DSM 467T and its knock-out mutant strain that is missing two polyhydroxyalkanoate synthases PhaC1 and PhaC2. During this first genome-wide expression study of R. rubrum, we were able to characterize cultivation-driven transcriptomic changes and to annotate non-coding elements as small RNAs. ER -
JURECKOVA, Katerina, Marketa NYKRYNOVA, Eva SLANINOVA, Hugo FLEURIOT-BLITMAN, Veronique AMSTUTZ, Kristyna HERMANKOVA, Matěj BEZDÍČEK, Katerina MRAZOVA, Kamila HRUBANOVA, Manfred ZINN, Stanislav OBRUCA and Karel SEDLAR. Cultivation driven transcriptomic changes in the wild-type and mutant strains of Rhodospirillum rubrum. \textit{Computational and Structural Biotechnology Journal}. AMSTERDAM: Elsevier, 2024, vol.~23, December 2024, p.~2681-2694. ISSN~2001-0370. Available from: https://dx.doi.org/10.1016/j.csbj.2024.06.023.
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