J 2024

The immunogenicity of p24 protein from HIV-1 virus is strongly supported and modulated by coupling with liposomes and mannan

ZACHOVA, K., E. BARTHELDYOVA, F. HUBATKA, M. KRUPKA, N. ODEHNALOVA et. al.

Basic information

Original name

The immunogenicity of p24 protein from HIV-1 virus is strongly supported and modulated by coupling with liposomes and mannan

Authors

ZACHOVA, K. (203 Czech Republic), E. BARTHELDYOVA (203 Czech Republic), F. HUBATKA (203 Czech Republic), M. KRUPKA (203 Czech Republic), N. ODEHNALOVA (203 Czech Republic), P. TURANEK KNOTIGOVA, Naděžda VAŠKOVICOVÁ (203 Czech Republic, belonging to the institution), K. SLOUPENSKA (203 Czech Republic), R. HROMADKA (203 Czech Republic), E. PAULOVICOVA (703 Slovakia), R. EFFENBERG (203 Czech Republic), M. LEDVINAE (203 Czech Republic), M. RASKA (203 Czech Republic) and J. TURANEKA (203 Czech Republic)

Edition

Carbohydrate Polymers, London, ELSEVIER SCI LTD, 2024, 0144-8617

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30102 Immunology

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

Impact factor

Impact factor: 11.200 in 2022

Organization unit

Faculty of Medicine

UT WoS

001183883300001

Keywords in English

Liposomes; Mannan; Recombinant protein; Adjuvants; Bioconjugation; Oxime ligation

Tags

Tags

International impact, Reviewed
Změněno: 16/8/2024 07:38, Mgr. Tereza Miškechová

Abstract

V originále

Anti-viral and anti-tumor vaccines aim to induce cytotoxic CD8+ T cells (CTL) and antibodies. Conserved protein antigens, such as p24 from human immunodeficiency virus, represent promising component for elicitation CTLs, nevertheless with suboptimal immunogenicity, if formulated as recombinant protein. To enhance immunogenicity and CTL response, recombinant proteins may be targeted to dendritic cells (DC) for cross presentation on MHCI, where mannose receptor and/or other lectin receptors could play an important role. Here, we constructed liposomal carrier-based vaccine composed of recombinant p24 antigen bound by metallochelating linkage onto surface of nanoliposomes with surface mannans coupled by aminooxy ligation. Generated mannosylated proteonanoliposomes were analyzed by dynamic light scattering, isothermal titration, and electron microscopy. Using murine DC line MutuDC and murine bone marrow derived DC (BMDC) we evaluated their immunogenicity and immunomodulatory activity. We show that p24 mannosylated proteonanoliposomes activate DC for enhanced MHCI, MHCII and CD40, CD80, and CD86 surface expression both on MutuDC and BMDC. p24 mannosylated liposomes were internalized by MutuDC with p24 intracellular localization within 1 to 3 h. The combination of metallochelating and aminooxy ligation could be used simultaneously to generate nanoliposomal adjuvanted recombinant protein-based vaccines versatile for combination of recombinant antigens relevant for antibody and CTL elicitation.