Developmental regulation of the maize Zm-p60.1 gene encoding a
beta-glucosidase located to plastids

J 2000

Developmental regulation of the maize Zm-p60.1 gene encoding a beta-glucosidase located to plastids

KRISTOFFERSEN, Peter, Břetislav BRZOBOHATÝ, Ingo HÖHFELD, Laszlo BAKO, Michael MELKONIAN et. al.

Basic information

Original name

Developmental regulation of the maize Zm-p60.1 gene encoding a beta-glucosidase located to plastids

Authors

KRISTOFFERSEN, Peter, Břetislav BRZOBOHATÝ, Ingo HÖHFELD, Laszlo BAKO, Michael MELKONIAN and Klaus PALME

Edition

Planta, Springer Berlin Heidelberg 2000, 2000, 0032-0935

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10600 1.6 Biological sciences

Country of publisher

Germany

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 3.199

RIV identification code

RIV/00216224:14310/00:00001867

Organization unit

Faculty of Science

Keywords in English

cytokinin - Cytokinin conjugate - -Glucosidase - Immunolocalization - Zea

Změněno: 7/12/2001 16:08, prof. RNDr. Břetislav Brzobohatý, CSc.

Abstract

V originále

A beta-glucosidase that cleaves the biologically inactive hormone conjugates cytokinin-O- and kinetin-N3-glucosides is encoded by the maize Zm-p60.1 gene. The expression of the Zm-p60.1 gene was analyzed by Northern blot analysis and in-situ hybridization. It was found that the expression levels of the Zm-p60.1-specific mRNA changed after pollination of carpellate inflorescences. The Zm-p60.1 c DNA was expressed in E. coli and antibodies were raised against the protein. An antibody was used to determine the tissue-specific localization of this protein. By in situ immunolocalization experiments, this protein was found to be located in cell layers below the epidermis and around the vascular bundles of the coleoptile. In the primary leaf, the Zm-p60.1 protein was detected in cells of the outermost cell layer and arround the vascular tissue. In floral tissue, Zm-p60.1 was present in the glumes, the carpels and in the outer cell layer of the style. In the coleoptiles, as determined by immuno-electronmicroscopy, the Zm-p60.1 protein was located exclusively in the plastids.

Links

VS96096, research and development project

Name: Laboratoř molekulární fyziologie rostlin

Investor: Ministry of Education, Youth and Sports of the CR, Laboratory of Molecular Plant Physiology

Citovat

KRISTOFFERSEN, Peter, Břetislav BRZOBOHATÝ, Ingo HÖHFELD, Laszlo BAKO, Michael MELKONIAN and Klaus PALME. Developmental regulation of the maize Zm-p60.1 gene encoding a beta-glucosidase located to plastids. Planta. Springer Berlin Heidelberg 2000, 2000, vol. 210, No 3, p. 407-415. ISSN 0032-0935.

@article{242591,
   author = {Kristoffersen, Peter and Brzobohatý, Břetislav and Höhfeld, Ingo and Bako, Laszlo and Melkonian, Michael and Palme, Klaus},
   article_number = {3},
   keywords = {cytokinin - Cytokinin conjugate - -Glucosidase - Immunolocalization - Zea},
   language = {eng},
   issn = {0032-0935},
   journal = {Planta},
   title = {Developmental regulation of the maize Zm-p60.1 gene encoding a beta-glucosidase located to plastids},
   volume = {210},
   year = {2000}
}

TY  - JOUR
ID  - 242591
AU  - Kristoffersen, Peter - Brzobohatý, Břetislav - Höhfeld, Ingo - Bako, Laszlo - Melkonian, Michael - Palme, Klaus
PY  - 2000
TI  - Developmental regulation of the maize Zm-p60.1 gene encoding a beta-glucosidase located to plastids
JF  - Planta
VL  - 210
IS  - 3
SP  - 407
EP  - 407
PB  - Springer Berlin Heidelberg 2000
SN  - 00320935
KW  - cytokinin - Cytokinin conjugate - -Glucosidase - Immunolocalization - Zea
N2  - A beta-glucosidase that cleaves the biologically inactive hormone conjugates cytokinin-O- and kinetin-N3-glucosides is encoded by the maize Zm-p60.1 gene. The expression of the Zm-p60.1 gene was analyzed by Northern blot analysis and in-situ hybridization. It was found that the expression levels of the Zm-p60.1-specific mRNA changed after pollination of carpellate inflorescences. The Zm-p60.1 c DNA was expressed in E. coli and antibodies were raised against the protein. An antibody was used to determine the tissue-specific localization of this protein. By in situ immunolocalization experiments, this protein was found to be located in cell layers below the epidermis and around the vascular bundles of the coleoptile. In the primary leaf, the Zm-p60.1 protein was detected in cells of the outermost cell layer and arround the vascular tissue. In floral tissue, Zm-p60.1 was present in the glumes, the carpels and in the outer cell layer of the style. In the coleoptiles, as determined by immuno-electronmicroscopy, the Zm-p60.1 protein was located exclusively in the plastids.
ER  -

KRISTOFFERSEN, Peter, Břetislav BRZOBOHATÝ, Ingo HÖHFELD, Laszlo BAKO, Michael MELKONIAN and Klaus PALME. Developmental regulation of the maize Zm-p60.1 gene encoding a beta-glucosidase located to plastids. \textit{Planta}. Springer Berlin Heidelberg 2000, 2000, vol.~210, No~3, p.~407-415. ISSN~0032-0935.

Detailed Information on Publication Record