MALÁ, Katrina Leslie Chavez, Jan SKALÁK, Elena ZEMLYANSKAYA, Vladislav DOLGIKH, Veronika JEDLIČKOVÁ, Helene ROBERT BOISIVON, Lenka HAVLÍČKOVÁ, Klára PANZAROVÁ, Panzarová TRTÍLEK, Ian BANCROFT and Jan HEJÁTKO. Primary multistep phosphorelay activation comprises both cytokinin and abiotic stress responses: insights from comparative analysis of Brassica type-A response regulators. Journal of experimental botany. England: OXFORD UNIV PRESSGREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND, 2024, 23 pp. ISSN 0022-0957. Available from: https://dx.doi.org/10.1093/jxb/erae335.
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Basic information
Original name Primary multistep phosphorelay activation comprises both cytokinin and abiotic stress responses: insights from comparative analysis of Brassica type-A response regulators
Authors MALÁ, Katrina Leslie Chavez, Jan SKALÁK, Elena ZEMLYANSKAYA, Vladislav DOLGIKH, Veronika JEDLIČKOVÁ, Helene ROBERT BOISIVON, Lenka HAVLÍČKOVÁ, Klára PANZAROVÁ, Panzarová TRTÍLEK, Ian BANCROFT and Jan HEJÁTKO.
Edition Journal of experimental botany, England, OXFORD UNIV PRESSGREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND, 2024, 0022-0957.
Other information
Original language English
Type of outcome Article in a journal
Field of Study 10611 Plant sciences, botany
Country of publisher United Kingdom of Great Britain and Northern Ireland
Confidentiality degree is not subject to a state or trade secret
WWW URL
Impact factor Impact factor: 6.900 in 2022
Organization unit Central European Institute of Technology
Doi http://dx.doi.org/10.1093/jxb/erae335
Keywords in English Arabidopsis thaliana; Brassica napus; Brassica oleracea; Brassica rapa; cytokinins; multistep phosphorelay; osmotic stress; salinity; two-component signaling; type-A response regulator
Tags CF CELLIM, CF PLANT
Tags International impact, Reviewed
Changed by Changed by: Mgr. Eva Dubská, učo 77638. Changed: 2/9/2024 21:50.
Abstract
Multistep phosphorelay (MSP) signaling integrates hormonal and environmental signals to control both plant development and adaptive responses. The type-A RESPONSE REGULATORs (RRAs), the downstream members of the MSP cascade and cytokinin primary response genes are supposed to mediate primarily the negative feedback regulation of (cytokinin-induced) MSP signaling. However, the transcriptional data suggest the involvement of RRAs in stress-related responses as well. By employing evolutionary conservation with the well-characterized Arabidopsis thaliana RRAs, we identified 5 and 38 novel putative RRAs in Brassica oleracea and Brassica napus, respectively. Our phylogenetic analysis suggests the existence of gene-specific selective pressure, maintaining the homologs of ARR3, ARR6, and ARR16 as singletons during the evolution of Brassicaceae. We categorized RRAs based on the kinetics of their cytokinin-mediated upregulation and observed both similarities and specificities in this type of response across Brassicaceae species. Using bioinformatic analysis and experimental data demonstrating the cytokinin and abiotic stress responsiveness of A. thaliana-derived TCSv2 reporter, we unveil the mechanistic conservation of cytokinin- and stress-mediated upregulation of RRAs in Brassica rapa and Brassica napus. Notably, we identify partial cytokinin dependency of cold stress-induced RRA transcription, thus corroborating the role of cytokinin signaling in the crop adaptive responses.
Links
CZ.02.01.01/00/22_008/0004581, interní kód MUName: Nové poznatky pro plodiny nové generace (TANGENC)
Investor: Ministry of Education, Youth and Sports of the CR, TowArds Next GENeration Crops (TANGENC), Priority 1 - Research and development
PrintDisplayed: 8/10/2024 01:17