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@article{2428241, author = {Malá, Katrina Leslie Chavez and Skalák, Jan and Zemlyanskaya, Elena and Dolgikh, Vladislav and Jedličková, Veronika and Robert Boisivon, Helene and Havlíčková, Lenka and Panzarová, Klára and Trtílek, Panzarová and Bancroft, Ian and Hejátko, Jan}, article_location = {England}, doi = {http://dx.doi.org/10.1093/jxb/erae335}, keywords = {Arabidopsis thaliana; Brassica napus; Brassica oleracea; Brassica rapa; cytokinins; multistep phosphorelay; osmotic stress; salinity; two-component signaling; type-A response regulator}, language = {eng}, issn = {0022-0957}, journal = {Journal of experimental botany}, title = {Primary multistep phosphorelay activation comprises both cytokinin and abiotic stress responses: insights from comparative analysis of Brassica type-A response regulators}, url = {https://watermark.silverchair.com/erae335.pdf?token=AQECAHi208BE49Ooan9kkhW_Ercy7Dm3ZL_9Cf3qfKAc485ysgAAA2QwggNgBgkqhkiG9w0BBwagggNRMIIDTQIBADCCA0YGCSqGSIb3DQEHATAeBglghkgBZQMEAS4wEQQMNyCYsUEbBZWAwkeqAgEQgIIDFxt-1N9lhylaG29NK2vZLUCuf3u9SwoK1h0_qEEMU4_Tug}, year = {2024} }
TY - JOUR ID - 2428241 AU - Malá, Katrina Leslie Chavez - Skalák, Jan - Zemlyanskaya, Elena - Dolgikh, Vladislav - Jedličková, Veronika - Robert Boisivon, Helene - Havlíčková, Lenka - Panzarová, Klára - Trtílek, Panzarová - Bancroft, Ian - Hejátko, Jan PY - 2024 TI - Primary multistep phosphorelay activation comprises both cytokinin and abiotic stress responses: insights from comparative analysis of Brassica type-A response regulators JF - Journal of experimental botany PB - OXFORD UNIV PRESSGREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN - 00220957 KW - Arabidopsis thaliana KW - Brassica napus KW - Brassica oleracea KW - Brassica rapa KW - cytokinins KW - multistep phosphorelay KW - osmotic stress KW - salinity KW - two-component signaling KW - type-A response regulator UR - https://watermark.silverchair.com/erae335.pdf?token=AQECAHi208BE49Ooan9kkhW_Ercy7Dm3ZL_9Cf3qfKAc485ysgAAA2QwggNgBgkqhkiG9w0BBwagggNRMIIDTQIBADCCA0YGCSqGSIb3DQEHATAeBglghkgBZQMEAS4wEQQMNyCYsUEbBZWAwkeqAgEQgIIDFxt-1N9lhylaG29NK2vZLUCuf3u9SwoK1h0_qEEMU4_Tug N2 - Multistep phosphorelay (MSP) signaling integrates hormonal and environmental signals to control both plant development and adaptive responses. The type-A RESPONSE REGULATORs (RRAs), the downstream members of the MSP cascade and cytokinin primary response genes are supposed to mediate primarily the negative feedback regulation of (cytokinin-induced) MSP signaling. However, the transcriptional data suggest the involvement of RRAs in stress-related responses as well. By employing evolutionary conservation with the well-characterized Arabidopsis thaliana RRAs, we identified 5 and 38 novel putative RRAs in Brassica oleracea and Brassica napus, respectively. Our phylogenetic analysis suggests the existence of gene-specific selective pressure, maintaining the homologs of ARR3, ARR6, and ARR16 as singletons during the evolution of Brassicaceae. We categorized RRAs based on the kinetics of their cytokinin-mediated upregulation and observed both similarities and specificities in this type of response across Brassicaceae species. Using bioinformatic analysis and experimental data demonstrating the cytokinin and abiotic stress responsiveness of A. thaliana-derived TCSv2 reporter, we unveil the mechanistic conservation of cytokinin- and stress-mediated upregulation of RRAs in Brassica rapa and Brassica napus. Notably, we identify partial cytokinin dependency of cold stress-induced RRA transcription, thus corroborating the role of cytokinin signaling in the crop adaptive responses. ER -
MALÁ, Katrina Leslie Chavez, Jan SKALÁK, Elena ZEMLYANSKAYA, Vladislav DOLGIKH, Veronika JEDLIČKOVÁ, Helene ROBERT BOISIVON, Lenka HAVLÍČKOVÁ, Klára PANZAROVÁ, Panzarová TRTÍLEK, Ian BANCROFT and Jan HEJÁTKO. Primary multistep phosphorelay activation comprises both cytokinin and abiotic stress responses: insights from comparative analysis of Brassica type-A response regulators. \textit{Journal of experimental botany}. England: OXFORD UNIV PRESSGREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND, 2024, 23 pp. ISSN~0022-0957. Available from: https://dx.doi.org/10.1093/jxb/erae335.
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