Region-specific gene expression profiling of early mouse
mandible uncovered SATB2 as a key molecule for teeth patterning

J 2024

Region-specific gene expression profiling of early mouse mandible uncovered SATB2 as a key molecule for teeth patterning

NEVORÁNKOVÁ, Petra, Marie ŠULCOVÁ, Michaela KAVKOVA, David ZIMČÍK, Simona MORAVCOVA BALKOVA et. al.

Basic information

Original name

Region-specific gene expression profiling of early mouse mandible uncovered SATB2 as a key molecule for teeth patterning

Authors

NEVORÁNKOVÁ, Petra (203 Czech Republic, belonging to the institution), Marie ŠULCOVÁ (203 Czech Republic, belonging to the institution), Michaela KAVKOVA (203 Czech Republic), David ZIMČÍK (203 Czech Republic, belonging to the institution), Simona MORAVCOVA BALKOVA (203 Czech Republic), Kristyna PELESKOVA (203 Czech Republic), Daniela KRISTEKOVÁ (703 Slovakia, belonging to the institution), Veronika JAKESOVA (203 Czech Republic), Tomas ZIKMUND (203 Czech Republic), Jozef KAISER (203 Czech Republic), Lydie IZAKOVIČOVÁ HOLLÁ (203 Czech Republic, belonging to the institution), Michal KOLAR (203 Czech Republic) and Marcela BUCHTOVÁ (203 Czech Republic, belonging to the institution)

Edition

Scientific Reports, Berlin, NATURE RESEARCH, 2024, 2045-2322

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30208 Dentistry, oral surgery and medicine

Country of publisher

Germany

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 4.600 in 2022

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.1038/s41598-024-68016-3

UT WoS

001285457700062

Keywords in English

Lower jaw; Incisor; Molar; Mouse; Microarray; Satb2

Abstract

V originále

Mammalian dentition exhibits distinct heterodonty, with more simple teeth located in the anterior area of the jaw and more complex teeth situated posteriorly. While some region-specific differences in signalling have been described previously, here we performed a comprehensive analysis of gene expression at the early stages of odontogenesis to obtain complete knowledge of the signalling pathways involved in early jaw patterning. Gene expression was analysed separately on anterior and posterior areas of the lower jaw at two early stages (E11.5 and E12.5) of odontogenesis. Gene expression profiling revealed distinct region-specific expression patterns in mouse mandibles, including several known BMP and FGF signalling members and we also identified several new molecules exhibiting significant differences in expression along the anterior-posterior axis, which potentially can play the role during incisor and molar specification. Next, we followed one of the anterior molecules, SATB2, which was expressed not only in the anterior mesenchyme where incisor germs are initiated, however, we uncovered a distinct SATB2-positive region in the mesenchyme closely surrounding molars. Satb2-deficient animals demonstrated defective incisor development confirming a crucial role of SATB2 in formation of anterior teeth. On the other hand, ectopic tooth germs were observed in the molar area indicating differential effect of Satb2-deficiency in individual jaw regions. In conclusion, our data provide a rich source of fundamental information, which can be used to determine molecular regulation driving early embryonic jaw patterning and serve for a deeper understanding of molecular signalling directed towards incisor and molar development.

Links

LM2018129, research and development project

Name: Národní infrastruktura pro biologické a medicínské zobrazování Czech-BioImaging

Investor: Ministry of Education, Youth and Sports of the CR

LM2023055, research and development project

Name: Česká národní infrastruktura pro biologická data

Investor: Ministry of Education, Youth and Sports of the CR, ELIXIR-CZ: Czech National Infrastructure for Biological Data

Citovat

NEVORÁNKOVÁ, Petra, Marie ŠULCOVÁ, Michaela KAVKOVA, David ZIMČÍK, Simona MORAVCOVA BALKOVA, Kristyna PELESKOVA, Daniela KRISTEKOVÁ, Veronika JAKESOVA, Tomas ZIKMUND, Jozef KAISER, Lydie IZAKOVIČOVÁ HOLLÁ, Michal KOLAR and Marcela BUCHTOVÁ. Region-specific gene expression profiling of early mouse mandible uncovered SATB2 as a key molecule for teeth patterning. Scientific Reports. Berlin: NATURE RESEARCH, 2024, vol. 14, No 1, p. 1-19. ISSN 2045-2322. Available from: https://dx.doi.org/10.1038/s41598-024-68016-3.

@article{2430459,
   author = {Nevoránková, Petra and Šulcová, Marie and Kavkova, Michaela and Zimčík, David and Moravcova Balkova, Simona and Peleskova, Kristyna and Kristeková, Daniela and Jakesova, Veronika and Zikmund, Tomas and Kaiser, Jozef and Izakovičová Hollá, Lydie and Kolar, Michal and Buchtová, Marcela},
   article_location = {Berlin},
   article_number = {1},
   doi = {http://dx.doi.org/10.1038/s41598-024-68016-3},
   keywords = {Lower jaw; Incisor; Molar; Mouse; Microarray; Satb2},
   language = {eng},
   issn = {2045-2322},
   journal = {Scientific Reports},
   title = {Region-specific gene expression profiling of early mouse mandible uncovered SATB2 as a key molecule for teeth patterning},
   url = {https://www.nature.com/articles/s41598-024-68016-3},
   volume = {14},
   year = {2024}
}

TY  - JOUR
ID  - 2430459
AU  - Nevoránková, Petra - Šulcová, Marie - Kavkova, Michaela - Zimčík, David - Moravcova Balkova, Simona - Peleskova, Kristyna - Kristeková, Daniela - Jakesova, Veronika - Zikmund, Tomas - Kaiser, Jozef - Izakovičová Hollá, Lydie - Kolar, Michal - Buchtová, Marcela
PY  - 2024
TI  - Region-specific gene expression profiling of early mouse mandible uncovered SATB2 as a key molecule for teeth patterning
JF  - Scientific Reports
VL  - 14
IS  - 1
SP  - 1-19
EP  - 1-19
PB  - NATURE RESEARCH
SN  - 20452322
KW  - Lower jaw
KW  - Incisor
KW  - Molar
KW  - Mouse
KW  - Microarray
KW  - Satb2
UR  - https://www.nature.com/articles/s41598-024-68016-3
N2  - Mammalian dentition exhibits distinct heterodonty, with more simple teeth located in the anterior area of the jaw and more complex teeth situated posteriorly. While some region-specific differences in signalling have been described previously, here we performed a comprehensive analysis of gene expression at the early stages of odontogenesis to obtain complete knowledge of the signalling pathways involved in early jaw patterning. Gene expression was analysed separately on anterior and posterior areas of the lower jaw at two early stages (E11.5 and E12.5) of odontogenesis. Gene expression profiling revealed distinct region-specific expression patterns in mouse mandibles, including several known BMP and FGF signalling members and we also identified several new molecules exhibiting significant differences in expression along the anterior-posterior axis, which potentially can play the role during incisor and molar specification. Next, we followed one of the anterior molecules, SATB2, which was expressed not only in the anterior mesenchyme where incisor germs are initiated, however, we uncovered a distinct SATB2-positive region in the mesenchyme closely surrounding molars. Satb2-deficient animals demonstrated defective incisor development confirming a crucial role of SATB2 in formation of anterior teeth. On the other hand, ectopic tooth germs were observed in the molar area indicating differential effect of Satb2-deficiency in individual jaw regions. In conclusion, our data provide a rich source of fundamental information, which can be used to determine molecular regulation driving early embryonic jaw patterning and serve for a deeper understanding of molecular signalling directed towards incisor and molar development.
ER  -

NEVORÁNKOVÁ, Petra, Marie ŠULCOVÁ, Michaela KAVKOVA, David ZIMČÍK, Simona MORAVCOVA BALKOVA, Kristyna PELESKOVA, Daniela KRISTEKOVÁ, Veronika JAKESOVA, Tomas ZIKMUND, Jozef KAISER, Lydie IZAKOVIČOVÁ HOLLÁ, Michal KOLAR and Marcela BUCHTOVÁ. Region-specific gene expression profiling of early mouse mandible uncovered SATB2 as a key molecule for teeth patterning. \textit{Scientific Reports}. Berlin: NATURE RESEARCH, 2024, vol.~14, No~1, p.~1-19. ISSN~2045-2322. Available from: https://dx.doi.org/10.1038/s41598-024-68016-3.

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