Unveiling vertebrate development dynamics in frog Xenopus
laevis using micro-CT imaging

J 2024

Unveiling vertebrate development dynamics in frog Xenopus laevis using micro-CT imaging

LÁZŇOVSKÝ, Jakub, Michaela KAVKOVÁ, Alice Helena REIS, Pavla ROBOVSKA-HAVELKOVA, Lorena AGOSTINI MAIA et. al.

Basic information

Original name

Unveiling vertebrate development dynamics in frog Xenopus laevis using micro-CT imaging

Authors

LÁZŇOVSKÝ, Jakub (203 Czech Republic), Michaela KAVKOVÁ (203 Czech Republic), Alice Helena REIS, Pavla ROBOVSKA-HAVELKOVA (203 Czech Republic), Lorena AGOSTINI MAIA (76 Brazil), Jan KŘIVÁNEK (203 Czech Republic, belonging to the institution), Tomas ZIKMUND (203 Czech Republic), Jozef KAISER (203 Czech Republic), Marcela BUCHTOVÁ (203 Czech Republic, belonging to the institution) and Jakub HARNOŠ (203 Czech Republic, guarantor, belonging to the institution)

Edition

GigaScience, OXFORD, Oxford University Press, 2024, 2047-217X

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10605 Developmental biology

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 9.200 in 2022

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.1093/gigascience/giae037

UT WoS

001286029900001

Keywords in English

Xenopus laevis; development; vertebrates; micro-computed tomography; morphological changes

Abstract

V originále

Background Xenopus laevis, the African clawed frog, is a versatile vertebrate model organism in various biological disciplines, prominently in developmental biology to study body plan reorganization during metamorphosis. However, a notable gap exists in the availability of comprehensive datasets encompassing Xenopus' late developmental stages.Findings This study utilized micro-computed tomography (micro-CT), a noninvasive 3-dimensional (3D) imaging technique with micrometer-scale resolution, to explore the developmental dynamics and morphological changes in Xenopus laevis. Our approach involved generating high-resolution images and computed 3D models of developing Xenopus specimens, spanning from premetamorphosis tadpoles to fully mature adults. This dataset enhances our understanding of vertebrate development and supports various analyses. We conducted a careful examination, analyzing body size, shape, and morphological features, focusing on skeletogenesis, teeth, and organs like the brain and gut at different stages. Our analysis yielded valuable insights into 3D morphological changes during Xenopus' development, documenting details previously unrecorded. These datasets hold the solid potential for further morphological and morphometric analyses, including segmentation of hard and soft tissues.Conclusions Our repository of micro-CT scans represents a significant resource that can enhance our understanding of Xenopus' development and the associated morphological changes in the future. The widespread utility of this amphibian species, coupled with the exceptional quality of our scans, which encompass a comprehensive series of developmental stages, opens up extensive opportunities for their broader research application. Moreover, these scans can be used in virtual reality, 3D printing, and educational contexts, further expanding their value and impact.

Links

GA22-02794S, research and development project

Name: Mechanorecepce jako mechanizmus řídící odontogenezi napříč obratlovci

Investor: Czech Science Foundation

GA22-06405S, research and development project

Name: Odhalení mechanismu formování nervové trubice

Investor: Czech Science Foundation

MUNI/J/0004/2021, interní kód MU

Name: Role of the Planar Cell Polarity and Mitochondria Crosstalk in Fuelling Cell Migration

Investor: Masaryk University, MASH JUNIOR - MUNI Award In Science and Humanities JUNIOR

90251, large research infrastructures

Name: CzechNanoLab II

Citovat

LÁZŇOVSKÝ, Jakub, Michaela KAVKOVÁ, Alice Helena REIS, Pavla ROBOVSKA-HAVELKOVA, Lorena AGOSTINI MAIA, Jan KŘIVÁNEK, Tomas ZIKMUND, Jozef KAISER, Marcela BUCHTOVÁ and Jakub HARNOŠ. Unveiling vertebrate development dynamics in frog Xenopus laevis using micro-CT imaging. GigaScience. OXFORD: Oxford University Press, 2024, vol. 13, JUNE, p. 1-16. ISSN 2047-217X. Available from: https://dx.doi.org/10.1093/gigascience/giae037.

@article{2430478,
   author = {Lázňovský, Jakub and Kavková, Michaela and Reis, Alice Helena and RobovskaandHavelkova, Pavla and Agostini Maia, Lorena and Křivánek, Jan and Zikmund, Tomas and Kaiser, Jozef and Buchtová, Marcela and Harnoš, Jakub},
   article_location = {OXFORD},
   article_number = {JUNE},
   doi = {http://dx.doi.org/10.1093/gigascience/giae037},
   keywords = {Xenopus laevis; development; vertebrates; micro-computed tomography; morphological changes},
   language = {eng},
   issn = {2047-217X},
   journal = {GigaScience},
   title = {Unveiling vertebrate development dynamics in frog Xenopus laevis using micro-CT imaging},
   url = {https://academic.oup.com/gigascience/article/doi/10.1093/gigascience/giae037/7714386?login=true},
   volume = {13},
   year = {2024}
}

TY  - JOUR
ID  - 2430478
AU  - Lázňovský, Jakub - Kavková, Michaela - Reis, Alice Helena - Robovska-Havelkova, Pavla - Agostini Maia, Lorena - Křivánek, Jan - Zikmund, Tomas - Kaiser, Jozef - Buchtová, Marcela - Harnoš, Jakub
PY  - 2024
TI  - Unveiling vertebrate development dynamics in frog Xenopus laevis using micro-CT imaging
JF  - GigaScience
VL  - 13
IS  - JUNE
SP  - 1-16
EP  - 1-16
PB  - Oxford University Press
SN  - 2047217X
KW  - Xenopus laevis
KW  - development
KW  - vertebrates
KW  - micro-computed tomography
KW  - morphological changes
UR  - https://academic.oup.com/gigascience/article/doi/10.1093/gigascience/giae037/7714386?login=true
N2  - Background Xenopus laevis, the African clawed frog, is a versatile vertebrate model organism in various biological disciplines, prominently in developmental biology to study body plan reorganization during metamorphosis. However, a notable gap exists in the availability of comprehensive datasets encompassing Xenopus' late developmental stages.Findings This study utilized micro-computed tomography (micro-CT), a noninvasive 3-dimensional (3D) imaging technique with micrometer-scale resolution, to explore the developmental dynamics and morphological changes in Xenopus laevis. Our approach involved generating high-resolution images and computed 3D models of developing Xenopus specimens, spanning from premetamorphosis tadpoles to fully mature adults. This dataset enhances our understanding of vertebrate development and supports various analyses. We conducted a careful examination, analyzing body size, shape, and morphological features, focusing on skeletogenesis, teeth, and organs like the brain and gut at different stages. Our analysis yielded valuable insights into 3D morphological changes during Xenopus' development, documenting details previously unrecorded. These datasets hold the solid potential for further morphological and morphometric analyses, including segmentation of hard and soft tissues.Conclusions Our repository of micro-CT scans represents a significant resource that can enhance our understanding of Xenopus' development and the associated morphological changes in the future. The widespread utility of this amphibian species, coupled with the exceptional quality of our scans, which encompass a comprehensive series of developmental stages, opens up extensive opportunities for their broader research application. Moreover, these scans can be used in virtual reality, 3D printing, and educational contexts, further expanding their value and impact.
ER  -

LÁZŇOVSKÝ, Jakub, Michaela KAVKOVÁ, Alice Helena REIS, Pavla ROBOVSKA-HAVELKOVA, Lorena AGOSTINI MAIA, Jan KŘIVÁNEK, Tomas ZIKMUND, Jozef KAISER, Marcela BUCHTOVÁ and Jakub HARNOŠ. Unveiling vertebrate development dynamics in frog Xenopus laevis using micro-CT imaging. \textit{GigaScience}. OXFORD: Oxford University Press, 2024, vol.~13, JUNE, p.~1-16. ISSN~2047-217X. Available from: https://dx.doi.org/10.1093/gigascience/giae037.

Detailed Information on Publication Record