| KANDRA, Mário, Tereza VÁŇOVÁ, Vincent Alexander JONGEN, Jakub POSPÍŠIL, Josef NOVAK, Václav CHOCHOLA, Tomas BURYSKA, Zbyněk PROKOP, Zdenek HODNY, Aleš HAMPL, Dáša BOHAČIAKOVÁ and Josef JAROŠ. A closed 3D printed microfluidic device for automated growth and differentiation of cerebral organoids from single-cell suspension. Biotechnology Journal. WEINHEIM: WILEY-V C H VERLAG GMBH, 2024, vol. 19, No 8, p. 1-17. ISSN 1860-6768. Available from: https://dx.doi.org/10.1002/biot.202400240. |
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@article{2431599,
author = {Kandra, Mário and Váňová, Tereza and Jongen, Vincent Alexander and Pospíšil, Jakub and Novak, Josef and Chochola, Václav and Buryska, Tomas and Prokop, Zbyněk and Hodny, Zdenek and Hampl, Aleš and Bohačiaková, Dáša and Jaroš, Josef},
article_location = {WEINHEIM},
article_number = {8},
doi = {http://dx.doi.org/10.1002/biot.202400240},
keywords = {3D cell culture; microfluidics; organoids; pluripotent stem cells; tissue engineering},
language = {eng},
issn = {1860-6768},
journal = {Biotechnology Journal},
title = {A closed 3D printed microfluidic device for automated growth and differentiation of cerebral organoids from single-cell suspension},
url = {https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/10.1002/biot.202400240},
volume = {19},
year = {2024}
}
TY - JOUR
ID - 2431599
AU - Kandra, Mário - Váňová, Tereza - Jongen, Vincent Alexander - Pospíšil, Jakub - Novak, Josef - Chochola, Václav - Buryska, Tomas - Prokop, Zbyněk - Hodny, Zdenek - Hampl, Aleš - Bohačiaková, Dáša - Jaroš, Josef
PY - 2024
TI - A closed 3D printed microfluidic device for automated growth and differentiation of cerebral organoids from single-cell suspension
JF - Biotechnology Journal
VL - 19
IS - 8
SP - 1-17
EP - 1-17
PB - WILEY-V C H VERLAG GMBH
SN - 18606768
KW - 3D cell culture
KW - microfluidics
KW - organoids
KW - pluripotent stem cells
KW - tissue engineering
UR - https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/10.1002/biot.202400240
N2 - The development of 3D organoids has provided a valuable tool for studying human tissue and organ development in vitro. Cerebral organoids, in particular, offer a unique platform for investigating neural diseases. However, current methods for generating cerebral organoids suffer from limitations such as labor-intensive protocols and high heterogeneity among organoids. To address these challenges, we present a microfluidic device designed to automate and streamline the formation and differentiation of cerebral organoids. The device utilizes microwells with two different shapes to promote the formation of a single aggregate per well and incorporates continuous medium flow for optimal nutrient exchange. In silico simulations supported the effectiveness of the microfluidic chip in replicating cellular microenvironments. Our results demonstrate that the microfluidic chip enables uniform growth of cerebral organoids, significantly reducing the hands-on time required for maintenance. Importantly, the performance of the microfluidic system is comparable to the standard 96-well plate format even when using half the amount of culture medium, and the resulting organoids exhibit substantially developed neuroepithelial buds and cortical structures. This study highlights the potential of custom-designed microfluidic technology in improving the efficiency of cerebral organoid culture.
ER -
KANDRA, Mário, Tereza VÁŇOVÁ, Vincent Alexander JONGEN, Jakub POSPÍŠIL, Josef NOVAK, Václav CHOCHOLA, Tomas BURYSKA, Zbyněk PROKOP, Zdenek HODNY, Aleš HAMPL, Dáša BOHAČIAKOVÁ and Josef JAROŠ. A closed 3D printed microfluidic device for automated growth and differentiation of cerebral organoids from single-cell suspension. \textit{Biotechnology Journal}. WEINHEIM: WILEY-V C H VERLAG GMBH, 2024, vol.~19, No~8, p.~1-17. ISSN~1860-6768. Available from: https://dx.doi.org/10.1002/biot.202400240.
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