Detailed Information on Publication Record
2024
Long-term zinc treatment alters the mechanical properties and metabolism of prostate cancer cells
NAVRÁTIL, Jiří, Monika KRATOCHVÍLOVÁ, Martina RAUDENSKÁ, Jan BALVAN, Tomáš VIČAR et. al.Basic information
Original name
Long-term zinc treatment alters the mechanical properties and metabolism of prostate cancer cells
Authors
NAVRÁTIL, Jiří (203 Czech Republic, belonging to the institution), Monika KRATOCHVÍLOVÁ (203 Czech Republic, belonging to the institution), Martina RAUDENSKÁ (203 Czech Republic, belonging to the institution), Jan BALVAN (203 Czech Republic, belonging to the institution), Tomáš VIČAR (203 Czech Republic, belonging to the institution), Kateřina PETRLÁKOVÁ (203 Czech Republic, belonging to the institution), Kanako SUZUKI (392 Japan, belonging to the institution), Lucie JADRNA (203 Czech Republic), Jiri BURSA (203 Czech Republic), Martin KRÄTER (276 Germany), Kyoohyun KIM (276 Germany), Michal MASAŘÍK (203 Czech Republic, belonging to the institution) and Jaromír GUMULEC (203 Czech Republic, belonging to the institution)
Edition
Cancer Cell International, London, BMC, 2024, 1475-2867
Other information
Language
English
Type of outcome
Článek v odborném periodiku
Field of Study
30204 Oncology
Country of publisher
United Kingdom of Great Britain and Northern Ireland
Confidentiality degree
není předmětem státního či obchodního tajemství
References:
Impact factor
Impact factor: 5.800 in 2022
Organization unit
Faculty of Medicine
UT WoS
001310159400002
Keywords in English
Mechanobiology; Zinc; Actin; Cytoskeleton; Mitochondria; Vimentin; Metabolism; Cancer
Tags
International impact, Reviewed
Změněno: 23/9/2024 13:01, Mgr. Tereza Miškechová
Abstract
V originále
The failure of intracellular zinc accumulation is a key process in prostate carcinogenesis. Although prostate cancer cells can accumulate zinc after long-term exposure, chronic zinc oversupply may accelerate prostate carcinogenesis or chemoresistance. Because cancer progression is associated with energetically demanding cytoskeletal rearrangements, we investigated the effect of long-term zinc presence on biophysical parameters, ATP production, and EMT characteristics of two prostate cancer cell lines (PC-3, 22Rv1). Prolonged exposure to zinc increased ATP production, spare respiratory capacity, and induced a response in PC-3 cells, characterized by remodeling of vimentin and a shift of cell dry mass density and caveolin-1 to the perinuclear region. This zinc-induced remodeling correlated with a greater tendency to maintain actin architecture despite inhibition of actin polymerization by cytochalasin. Zinc partially restored epithelial characteristics in PC-3 cells by decreasing vimentin expression and increasing E-cadherin. Nevertheless, the expression of E-cadherin remained lower than that observed in predominantly oxidative, low-invasive 22Rv1 cells. Following long-term zinc exposure, we observed an increase in cell stiffness associated with an increased refractive index in the perinuclear region and an increased mitochondrial content. The findings of the computational simulations indicate that the mechanical response cannot be attributed exclusively to alterations in cytoskeletal composition. This observation suggests the potential involvement of an additional, as yet unidentified, mechanical contributor. These findings indicate that long-term zinc exposure alters a group of cellular parameters towards an invasive phenotype, including an increase in mitochondrial number, ATP production, and cytochalasin resistance. Ultimately, these alterations are manifested in the biomechanical properties of the cells.
Links
| EF18_046/0015974, research and development project |
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| LM2018129, research and development project |
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| LM2023042, research and development project |
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| MUNI/A/1587/2023, interní kód MU |
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| NU22J-08-00062, research and development project |
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| 90254, large research infrastructures |
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| 964997, interní kód MU |
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