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@article{2433137,
author = {Navrátilová, Polina and Wojtas, Daniel and Wójcik, Anna and Ullattil, Sanjay Gopal and Loja, Tomáš and Pávková Goldbergová, Monika},
article_location = {Washington},
doi = {http://dx.doi.org/10.1021/acsanm.4c03385},
keywords = {MoO3; macrophages; cytotoxicity; reactive oxygen species; oxidative stress},
language = {eng},
issn = {2574-0970},
journal = {ACS Applied Nano Materials},
title = {Dual Nature of MoO3 Particles in Human-Derived Macrophage Milieu},
url = {https://pubs.acs.org/doi/full/10.1021/acsanm.4c03385},
year = {2024}
}
TY - JOUR
ID - 2433137
AU - Navrátilová, Polina - Wojtas, Daniel - Wójcik, Anna - Ullattil, Sanjay Gopal - Loja, Tomáš - Pávková Goldbergová, Monika
PY - 2024
TI - Dual Nature of MoO3 Particles in Human-Derived Macrophage Milieu
JF - ACS Applied Nano Materials
PB - American Chemical Society
SN - 25740970
KW - MoO3
KW - macrophages
KW - cytotoxicity
KW - reactive oxygen species
KW - oxidative stress
UR - https://pubs.acs.org/doi/full/10.1021/acsanm.4c03385
N2 - Molybdenum trioxide (MoO3) has over the years captivated significant scientific attention, mainly in infection prevention and cancer treatment. However, as cytotoxicity of the compound poses a great concern, herein we test the hypothesis that MoO3 in the form of commercially available particles triggers cytotoxic responses yet may also act as an antioxidant. In doing so, human-derived macrophages were exposed to variously concentrated (50–500 μM) MoO3 particle suspensions and examined from the cytocompatibility point of view. Cytotoxicity assays were coupled with cell morphology characterization via confocal laser scanning microscopy imaging, reactive oxygen species (ROS) detection, and flow cytometry for macrophage polarization analysis. Moreover, the DPPH assay for radical scavenging ability was performed. Generally, dose-dependent cytotoxicity of MoO3 was noted, with concentrations up to 100 μM being well-tolerated by macrophages. The particles demonstrated antioxidant activity, as confirmed by their ability to scavenge DPPH radicals. However, at the highest concentration studied, i.e., 500 μM, MoO3 became highly cytotoxic and triggered cell death, as evidenced by alterations in cell shape and abnormal ROS production. Overall, the antioxidative and cytotoxic character of MoO3 particles at low and high concentrations, respectively, was highlighted. The findings pave the way for future studies in the area of MoO3-based materials and validate their usefulness for biomedical purposes, as in antioxidant-based treatments.
ER -
NAVRÁTILOVÁ, Polina, Daniel WOJTAS, Anna WÓJCIK, Sanjay Gopal ULLATTIL, Tomáš LOJA and Monika PÁVKOVÁ GOLDBERGOVÁ. Dual Nature of MoO3 Particles in Human-Derived Macrophage Milieu. \textit{ACS Applied Nano Materials}. Washington: American Chemical Society, 2024. ISSN~2574-0970. Available from: https://dx.doi.org/10.1021/acsanm.4c03385.
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