Dual Nature of MoO3 Particles in Human-Derived Macrophage
Milieu

J 2024

Dual Nature of MoO3 Particles in Human-Derived Macrophage Milieu

NAVRÁTILOVÁ, Polina, Daniel WOJTAS, Anna WÓJCIK, Sanjay Gopal ULLATTIL, Tomáš LOJA et. al.

Basic information

Original name

Dual Nature of MoO3 Particles in Human-Derived Macrophage Milieu

Authors

NAVRÁTILOVÁ, Polina, Daniel WOJTAS, Anna WÓJCIK, Sanjay Gopal ULLATTIL, Tomáš LOJA and Monika PÁVKOVÁ GOLDBERGOVÁ

Edition

ACS Applied Nano Materials, Washington, American Chemical Society, 2024, 2574-0970

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 5.900 in 2022

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.1021/acsanm.4c03385

UT WoS

001310564400001

Keywords in English

MoO3; macrophages; cytotoxicity; reactive oxygen species; oxidative stress

Abstract

V originále

Molybdenum trioxide (MoO3) has over the years captivated significant scientific attention, mainly in infection prevention and cancer treatment. However, as cytotoxicity of the compound poses a great concern, herein we test the hypothesis that MoO3 in the form of commercially available particles triggers cytotoxic responses yet may also act as an antioxidant. In doing so, human-derived macrophages were exposed to variously concentrated (50–500 μM) MoO3 particle suspensions and examined from the cytocompatibility point of view. Cytotoxicity assays were coupled with cell morphology characterization via confocal laser scanning microscopy imaging, reactive oxygen species (ROS) detection, and flow cytometry for macrophage polarization analysis. Moreover, the DPPH assay for radical scavenging ability was performed. Generally, dose-dependent cytotoxicity of MoO3 was noted, with concentrations up to 100 μM being well-tolerated by macrophages. The particles demonstrated antioxidant activity, as confirmed by their ability to scavenge DPPH radicals. However, at the highest concentration studied, i.e., 500 μM, MoO3 became highly cytotoxic and triggered cell death, as evidenced by alterations in cell shape and abnormal ROS production. Overall, the antioxidative and cytotoxic character of MoO3 particles at low and high concentrations, respectively, was highlighted. The findings pave the way for future studies in the area of MoO3-based materials and validate their usefulness for biomedical purposes, as in antioxidant-based treatments.

Links

LM2023050, research and development project

Name: Národní infrastruktura pro biologické a medicínské zobrazování

Investor: Ministry of Education, Youth and Sports of the CR, Czech BioImaging: National research infrastructure for biological and medical imaging

NU20-08-00149, research and development project

Name: Multicentrické hodnocení hypersenzitivní reakce u pacientů indikovaných k totální náhradě kloubu včetně hodnocení důvodů reimplanace

Investor: Ministry of Health of the CR, Subprogram 1 - standard

90251, large research infrastructures

Name: CzechNanoLab II

Citovat

NAVRÁTILOVÁ, Polina, Daniel WOJTAS, Anna WÓJCIK, Sanjay Gopal ULLATTIL, Tomáš LOJA and Monika PÁVKOVÁ GOLDBERGOVÁ. Dual Nature of MoO3 Particles in Human-Derived Macrophage Milieu. ACS Applied Nano Materials. Washington: American Chemical Society, 2024. ISSN 2574-0970. Available from: https://dx.doi.org/10.1021/acsanm.4c03385.

@article{2433137,
   author = {Navrátilová, Polina and Wojtas, Daniel and Wójcik, Anna and Ullattil, Sanjay Gopal and Loja, Tomáš and Pávková Goldbergová, Monika},
   article_location = {Washington},
   doi = {http://dx.doi.org/10.1021/acsanm.4c03385},
   keywords = {MoO3; macrophages; cytotoxicity; reactive oxygen species; oxidative stress},
   language = {eng},
   issn = {2574-0970},
   journal = {ACS Applied Nano Materials},
   title = {Dual Nature of MoO3 Particles in Human-Derived Macrophage Milieu},
   url = {https://pubs.acs.org/doi/full/10.1021/acsanm.4c03385},
   year = {2024}
}

TY  - JOUR
ID  - 2433137
AU  - Navrátilová, Polina - Wojtas, Daniel - Wójcik, Anna - Ullattil, Sanjay Gopal - Loja, Tomáš - Pávková Goldbergová, Monika
PY  - 2024
TI  - Dual Nature of MoO3 Particles in Human-Derived Macrophage Milieu
JF  - ACS Applied Nano Materials
PB  - American Chemical Society
SN  - 25740970
KW  - MoO3
KW  - macrophages
KW  - cytotoxicity
KW  - reactive oxygen species
KW  - oxidative stress
UR  - https://pubs.acs.org/doi/full/10.1021/acsanm.4c03385
N2  - Molybdenum trioxide (MoO3) has over the years captivated significant scientific attention, mainly in infection prevention and cancer treatment. However, as cytotoxicity of the compound poses a great concern, herein we test the hypothesis that MoO3 in the form of commercially available particles triggers cytotoxic responses yet may also act as an antioxidant. In doing so, human-derived macrophages were exposed to variously concentrated (50–500 μM) MoO3 particle suspensions and examined from the cytocompatibility point of view. Cytotoxicity assays were coupled with cell morphology characterization via confocal laser scanning microscopy imaging, reactive oxygen species (ROS) detection, and flow cytometry for macrophage polarization analysis. Moreover, the DPPH assay for radical scavenging ability was performed. Generally, dose-dependent cytotoxicity of MoO3 was noted, with concentrations up to 100 μM being well-tolerated by macrophages. The particles demonstrated antioxidant activity, as confirmed by their ability to scavenge DPPH radicals. However, at the highest concentration studied, i.e., 500 μM, MoO3 became highly cytotoxic and triggered cell death, as evidenced by alterations in cell shape and abnormal ROS production. Overall, the antioxidative and cytotoxic character of MoO3 particles at low and high concentrations, respectively, was highlighted. The findings pave the way for future studies in the area of MoO3-based materials and validate their usefulness for biomedical purposes, as in antioxidant-based treatments.
ER  -

NAVRÁTILOVÁ, Polina, Daniel WOJTAS, Anna WÓJCIK, Sanjay Gopal ULLATTIL, Tomáš LOJA and Monika PÁVKOVÁ GOLDBERGOVÁ. Dual Nature of MoO3 Particles in Human-Derived Macrophage Milieu. \textit{ACS Applied Nano Materials}. Washington: American Chemical Society, 2024. ISSN~2574-0970. Available from: https://dx.doi.org/10.1021/acsanm.4c03385.

Detailed Information on Publication Record