Histone Chaperone Deficiency in Arabidopsis Plants Triggers
Adaptive Epigenetic Changes in Histone Variants and
Modifications

J 2024

Histone Chaperone Deficiency in Arabidopsis Plants Triggers Adaptive Epigenetic Changes in Histone Variants and Modifications

FRANEK, Michal, Martina NEŠPOR DADEJOVÁ, Pavlína PÍREK, Karolína KRYŠTOFOVÁ, Tereza DOBISOVÁ et. al.

Basic information

Original name

Histone Chaperone Deficiency in Arabidopsis Plants Triggers Adaptive Epigenetic Changes in Histone Variants and Modifications

Authors

FRANEK, Michal, Martina NEŠPOR DADEJOVÁ, Pavlína PÍREK, Karolína KRYŠTOFOVÁ, Tereza DOBISOVÁ, Zbyněk ZDRÁHAL, Martina DVOŘÁČKOVÁ and Gabriela LOCHMANOVÁ

Edition

2024

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10611 Plant sciences, botany

Country of publisher

Netherlands

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Organization unit

Central European Institute of Technology

DOI

http://dx.doi.org/10.1016/j.mcpro.2024.100795

Keywords in English

Arabidopsis; chromatin remodeling; histone chaperone complex; histone variants; immunochemistry; mass spectrometry; post-translational modifications

Abstract

V originále

At the molecular scale, adaptive advantages during plant growth and development rely on modulation of gene expression, primarily provided by epigenetic machinery. One crucial part of this machinery is histone posttranslational modifications, which form a flexible system, driving transient changes in chromatin, and defining particular epigenetic states. Posttranslational modifications work in concert with replication-independent histone variants further adapted for transcriptional regulation and chromatin repair. However, little is known about how such complex regulatory pathways are orchestrated and interconnected in cells. In this work, we demonstrate the utility of mass spectrometry-based approaches to explore how different epigenetic layers interact in Arabidopsis mutants lacking certain histone chaperones. We show that defects in histone chaperone function (e.g., chromatin assembly factor-1 or nucleosome assembly protein 1 mutations) translate into an altered epigenetic landscape, which aids the plant in mitigating internal instability. We observe changes in both the levels and distribution of H2A.W.7, altogether with partial repurposing of H3.3 and changes in the key repressive (H3K27me1/2) or euchromatic marks (H3K36me1/2). These shifts in the epigenetic profile serve as a compensatory mechanism in response to impaired integration of the H3.1 histone in the fas1 mutants. Altogether, our findings suggest that maintaining genome stability involves a two-tiered approach. The first relies on flexible adjustments in histone marks, while the second level requires the assistance of chaperones for histone variant replacement.

Links

GA22-28190S, research and development project

Name: Komplexní charakterizace histonových epigenetických značek

Investor: Czech Science Foundation, Comprehensive characterization of histone epigenetic marks

GA23-06643S, research and development project

Name: Dispozice, výskyt a zpracování DNA lézí v genomu rostlin Arabidopsis thaliana defektních pro reparaci DNA a sestavování nukleozomů

Investor: Czech Science Foundation, Disposition, distribution, and processing of DNA lesions in the genome of Arabidopsis thaliana plants defective in DNA repair and nucleosome assembly

MUNI/R/1364/2023, interní kód MU

Name: Mechanizmy opravy DNA u rostliny Arabidopsis thaliana

Investor: Masaryk University, DNA repair mechanisms in plant Arabidopsis thaliana, CAREER RESTART

90254, large research infrastructures

Name: e-INFRA CZ II

Citovat

FRANEK, Michal, Martina NEŠPOR DADEJOVÁ, Pavlína PÍREK, Karolína KRYŠTOFOVÁ, Tereza DOBISOVÁ, Zbyněk ZDRÁHAL, Martina DVOŘÁČKOVÁ and Gabriela LOCHMANOVÁ. Histone Chaperone Deficiency in Arabidopsis Plants Triggers Adaptive Epigenetic Changes in Histone Variants and Modifications. 2024. Available from: https://dx.doi.org/10.1016/j.mcpro.2024.100795.

@article{2433977,
   author = {Franek, Michal and Nešpor Dadejová, Martina and Pírek, Pavlína and Kryštofová, Karolína and Dobisová, Tereza and Zdráhal, Zbyněk and Dvořáčková, Martina and Lochmanová, Gabriela},
   article_number = {7},
   doi = {http://dx.doi.org/10.1016/j.mcpro.2024.100795},
   keywords = {Arabidopsis; chromatin remodeling; histone chaperone complex; histone variants; immunochemistry; mass spectrometry; post-translational modifications},
   language = {eng},
   title = {Histone Chaperone Deficiency in Arabidopsis Plants Triggers Adaptive Epigenetic Changes in Histone Variants and Modifications},
   url = {https://www.mcponline.org/article/S1535-9476(24)00085-9/fulltext},
   volume = {23},
   year = {2024}
}

TY  - JOUR
ID  - 2433977
AU  - Franek, Michal - Nešpor Dadejová, Martina - Pírek, Pavlína - Kryštofová, Karolína - Dobisová, Tereza - Zdráhal, Zbyněk - Dvořáčková, Martina - Lochmanová, Gabriela
PY  - 2024
TI  - Histone Chaperone Deficiency in Arabidopsis Plants Triggers Adaptive Epigenetic Changes in Histone Variants and Modifications
VL  - 23
IS  - 7
SP  - 1-17
EP  - 1-17
KW  - Arabidopsis
KW  - chromatin remodeling
KW  - histone chaperone complex
KW  - histone variants
KW  - immunochemistry
KW  - mass spectrometry
KW  - post-translational modifications
UR  - https://www.mcponline.org/article/S1535-9476(24)00085-9/fulltext
N2  - At the molecular scale, adaptive advantages during plant growth and development rely on modulation of gene expression, primarily provided by epigenetic machinery. One crucial part of this machinery is histone posttranslational modifications, which form a flexible system, driving transient changes in chromatin, and defining particular epigenetic states. Posttranslational modifications work in concert with replication-independent histone variants further adapted for transcriptional regulation and chromatin repair. However, little is known about how such complex regulatory pathways are orchestrated and interconnected in cells. In this work, we demonstrate the utility of mass spectrometry-based approaches to explore how different epigenetic layers interact in Arabidopsis mutants lacking certain histone chaperones. We show that defects in histone chaperone function (e.g., chromatin assembly factor-1 or nucleosome assembly protein 1 mutations) translate into an altered epigenetic landscape, which aids the plant in mitigating internal instability. We observe changes in both the levels and distribution of H2A.W.7, altogether with partial repurposing of H3.3 and changes in the key repressive (H3K27me1/2) or euchromatic marks (H3K36me1/2). These shifts in the epigenetic profile serve as a compensatory mechanism in response to impaired integration of the H3.1 histone in the fas1 mutants. Altogether, our findings suggest that maintaining genome stability involves a two-tiered approach. The first relies on flexible adjustments in histone marks, while the second level requires the assistance of chaperones for histone variant replacement.
ER  -

FRANEK, Michal, Martina NEŠPOR DADEJOVÁ, Pavlína PÍREK, Karolína KRYŠTOFOVÁ, Tereza DOBISOVÁ, Zbyněk ZDRÁHAL, Martina DVOŘÁČKOVÁ and Gabriela LOCHMANOVÁ. \textit{Histone Chaperone Deficiency in Arabidopsis Plants Triggers Adaptive Epigenetic Changes in Histone Variants and Modifications}. 2024. Available from: https://dx.doi.org/10.1016/j.mcpro.2024.100795.

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