J 2024

Analysis of subcellular energy metabolism in five Lacertidae lizards across varied environmental conditions

ZAGAR, Anamarija, Urban DAJCMAN, Rodrigo MEGIA-PALMA, Tatjana SIMCIC, Frederico M. BARROSO et. al.

Basic information

Original name

Analysis of subcellular energy metabolism in five Lacertidae lizards across varied environmental conditions

Authors

ZAGAR, Anamarija (guarantor), Urban DAJCMAN, Rodrigo MEGIA-PALMA, Tatjana SIMCIC, Frederico M. BARROSO, Senka NAJMAN BAŠKIERA (191 Croatia, belonging to the institution) and Miguel A. CARRETERO

Edition

Comparative Biochemistry and Physiology -Part A : Molecular and Integrative Physiology, NEW YORK, ELSEVIER SCIENCE INC, 2024, 1095-6433

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10613 Zoology

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

Impact factor

Impact factor: 2.300 in 2022

Organization unit

Faculty of Science

DOI

UT WoS

001303253800001

Keywords in English

Physiology; Aerobic respiration; Lacertids; Interspecific variability; Hemoglobin

Tags

Tags

International impact, Reviewed
Změněno: 23/9/2024 13:03, Mgr. Lucie Jarošová, DiS.

Abstract

V originále

Aerobic respiration is the main energy source for most eukaryotes, and efficient mitochondrial energy transfer greatly influences organismal fitness. To survive environmental changes, cells have evolved to adjust their biochemistry. Thus, measuring energy metabolism at the subcellular level can enhance our understanding of individual performance, population dynamics, and species distribution ranges. We investigated three important metabolic traits at the subcellular level in five lacertid lizard species sampled from different elevations, from sea level up to 2000 m. We examined hemoglobin concentration, two markers of oxidative stress (catalase activity and carbonyl concentration) and maximum rate of metabolic respiration at the subcellular level (potential metabolic activity at the electron transport system). The traits were analysed in laboratory acclimated adult male lizards to investigate the adaptive metabolic responses to the variable environmental conditions at the local sampling sites. Potential metabolic activity at the cellular level was measured at four temperatures - 28 degrees C, 30 degrees C, 32 degrees C and 34 degrees C - covering the range of preferred body temperatures of the species studied. Hemoglobin content, carbonyl concentration and potential metabolic activity did not differ significantly among species. Interspecific differences were found in the catalase activity, Potential metabolic activity increased with temperature in parallel in all five species. The highest response of the metabolic rate with temperature (Q10) and Arrhenius activation energy (Ea) was recorded in the high-mountain species Iberolacerta monticola.