Detailed Information on Publication Record
2024
Unveiling molecular strategies of Heterorhabditis bacteriophora: a comparative analysis of in vitro activation and in vivo infection dynamics
ILGOVÁ, Jana, Sara ŠREIBR, Pavel DOBEŠ, Jiří VOREL, Jana HURYCHOVÁ et. al.Basic information
Original name
Unveiling molecular strategies of Heterorhabditis bacteriophora: a comparative analysis of in vitro activation and in vivo infection dynamics
Authors
ILGOVÁ, Jana (703 Slovakia, guarantor, belonging to the institution), Sara ŠREIBR (276 Germany, belonging to the institution), Pavel DOBEŠ (203 Czech Republic, belonging to the institution), Jiří VOREL (203 Czech Republic, belonging to the institution), Jana HURYCHOVÁ (203 Czech Republic, belonging to the institution), Jacek MARCINIAK (203 Czech Republic, belonging to the institution), Martin KAŠNÝ (203 Czech Republic, belonging to the institution) and Pavel HYRŠL (203 Czech Republic, belonging to the institution)
Edition
19th Meeting of the IOBC/WPRS. Working Group Microbial and Nematode Control of Invertebrate Pests, 2024
Other information
Language
English
Type of outcome
Konferenční abstrakt
Field of Study
10608 Biochemistry and molecular biology
Country of publisher
Portugal
Confidentiality degree
není předmětem státního či obchodního tajemství
References:
Organization unit
Faculty of Science
Keywords in English
Heterorhabditis bacteriophora; recovery; activation; transcriptomics
Změněno: 24/9/2024 10:14, Mgr. et Mgr. Jana Ilgová, Ph.D.
Abstract
V originále
The interplay between entomopathogenic nematodes (EPNs) and their hosts offers promising avenues for environmentally sustainable pest management strategies. Our research investigates the intricate molecular mechanisms underlying the infection dynamics of Heterorhabditis bacteriophora strain Az 148. Invasion tactics of this EPN species involve the active penetration of the host by the third-stage larvae, also called infective juveniles (IJs). Upon contact with the host or its molecules, IJs start the process called activation or recovery, which involves the transition from the dauer stage to the infective one. Shortly after the host colonization, IJs release symbiotic bacteria of the genus Photorhabdus, which induce fatal septicemia in the host. In laboratory settings, the recovery of IJs is often simulated by challenging them with various host-derived materials (such as tissue homogenate or hemolymph). Nevertheless, the extent to which in vitro activation corresponds to in vivo infection has not been elucidated. We are conducting a comparative RNA-seq analysis at various time points throughout the in vitro activation and in vivo infection of the greater wax moth larvae, Galleria mellonella. Our analysis focuses on five critical time points (3, 6, 9, 12, and 15 hours post-infection) to capture the dynamic changes in H. bacteriophora gene expression during IJs infection in vivo. In vitro activation involves exposure of IJs to G. mellonella-derived homogenates for three periods (3, 6 and 9 hours), simulating the interaction with host tissues. Recognizing the low recovery of IJs after in vivo infection, we employed a single-cell RNA NGS library preparation strategy, followed by sequencing at the DNBSEQ-G400 (MGI) platform using FCL PE100 kit. Differential expression analysis will identify key transcripts by mapping reads to a de novo assembled transcriptome. Our aim is to assess the correlation between H. bacteriophora gene expression patterns during in vitro activation and the dynamics observed during in vivo infection. This data will contribute to the understanding of H. bacteriophora molecular strategies employed in vivo and address the relevance of in vitro activation models in studying the infection process.
Links
| GA23-06457S, research and development project |
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