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@article{2439064,
author = {Dosedelova, Vera and Lastovickova, Marketa and Konečný, Štefan and Dolina, Jiří and Kuban, Petr},
article_location = {AMSTERDAM},
article_number = {November 2024},
doi = {http://dx.doi.org/10.1016/j.chroma.2024.465354},
keywords = {Barrett's esophagus; Bile acids; Reflux disease; Saliva; Saliva sampling methods},
language = {eng},
issn = {0021-9673},
journal = {Journal of Chromatography},
title = {Optimization of saliva sampling methods for analysis of bile acids by UHPLC-MS},
url = {https://www.sciencedirect.com/science/article/pii/S0021967324007283?via%3Dihub},
volume = {1736},
year = {2024}
}
TY - JOUR
ID - 2439064
AU - Dosedelova, Vera - Lastovickova, Marketa - Konečný, Štefan - Dolina, Jiří - Kuban, Petr
PY - 2024
TI - Optimization of saliva sampling methods for analysis of bile acids by UHPLC-MS
JF - Journal of Chromatography
VL - 1736
IS - November 2024
SP - 1-7
EP - 1-7
PB - Elsevier
SN - 00219673
KW - Barrett's esophagus
KW - Bile acids
KW - Reflux disease
KW - Saliva
KW - Saliva sampling methods
UR - https://www.sciencedirect.com/science/article/pii/S0021967324007283?via%3Dihub
N2 - This study investigated methods for sampling bile acids in saliva, a potential non-invasive diagnostic biofluid. Bile acids have been implicated in causing damage and permanent changes to the esophageal mucosa and increasing the risk of developing Barrett's esophagus, a condition that can potentially progress to esophageal cancer. Three saliva collection methods were compared: spitting, Salivette (R) swabs, and Salivette Cortisol (R) swabs. Spitting emerged as the superior method with the highest recoveries and the least interference, likely due to Salivette swabs retaining bile acids or introducing unknown interferences. All saliva samples were analyzed by UHPLC-MS/MS using the Zorbax RRHD Eclipse Plus C18 column (3 x 50 mm, 1.8 mu m) in gradient elution of 0.1 % formic acid in water and methanol. Saliva sample stability was assessed over 14 days, reflecting typical storage times. The levels of detected bile acids were stable for the measured period (RSD <= 22 %) and no degradation was observed. Bile acid levels in saliva fluctuated throughout the day, with the greatest changes observed for glycine-conjugated bile acids after meals. To minimize sampling variability, saliva collection by spitting after overnight fasting is recommended for future studies. Our findings are applicable for standardized bile acid sampling and are currently applied in a large clinical study evaluating bile acids as potential susceptibility markers for Barrett's esophagus diagnostics.
ER -
DOSEDELOVA, Vera, Marketa LASTOVICKOVA, Štefan KONEČNÝ, Jiří DOLINA and Petr KUBAN. Optimization of saliva sampling methods for analysis of bile acids by UHPLC-MS. \textit{Journal of Chromatography}. AMSTERDAM: Elsevier, 2024, vol.~1736, November 2024, p.~1-7. ISSN~0021-9673. Available from: https://dx.doi.org/10.1016/j.chroma.2024.465354.
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