Wildlife as Reservoirs of Encephalitozoon Cuniculi and
Encephalitozoon Hellem and Molecular Genotyping of
Encephalitozoon spp. in Small Mammals in the Czech Republic

J 2024

Wildlife as Reservoirs of Encephalitozoon Cuniculi and Encephalitozoon Hellem and Molecular Genotyping of Encephalitozoon spp. in Small Mammals in the Czech Republic

BÁRTOVÁ, Eva, Jiřina MARKOVÁ, Alena ŽÁKOVSKÁ, Zuzana ČADKOVÁ, Marie BUDÍKOVÁ et. al.

Základní údaje

Originální název

Wildlife as Reservoirs of Encephalitozoon Cuniculi and Encephalitozoon Hellem and Molecular Genotyping of Encephalitozoon spp. in Small Mammals in the Czech Republic

Autoři

BÁRTOVÁ, Eva, Jiřina MARKOVÁ, Alena ŽÁKOVSKÁ, Zuzana ČADKOVÁ a Marie BUDÍKOVÁ

Vydání

Acta Parasitologica, Springer, 2024, 1230-2821

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

40301 Veterinary science

Stát vydavatele

Švýcarsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 1.500 v roce 2022

Organizační jednotka

Přírodovědecká fakulta

DOI

http://dx.doi.org/10.1007/s11686-024-00920-0

Klíčová slova anglicky

Encephalitozoonosis; Wildlife; Urban area; Zoonosis; Genotyping

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 3. 10. 2024 15:35, Mgr. Marie Šípková, DiS.

Anotace

V originále

Purpose: Parasites of genus Encephalitozoon are well known pathogens of domestic animals however less attention was paid to its spread among wildlife that can play an important role of reservoir of infection. The aim of the study was to conduct molecular detection and genotype characterization of Encephalitozoon spp. in wild small mammals trapped in localities both near to and at a large distance from residential areas. Methods: In total, 300 wild small mammals (274 Rodentia and 26 Eulipotyphla) were trapped in 41 localities of the Czech Republic and tested by nested PCR for Encephalitozoon spp. Results: The DNA of Encephalitozoon spp. was proved in tissues (brain or liver) of 11% (32/300) of animals. There was a statistically significant difference (p < 0.001) in positivity among animal species with the most infected species Micromys minutus (50%, 4/8) and Myodes glareolus (17%, 9/53). There was also statistically significant difference (p < 0.001) between localities with the higher positivity (29%, 12/42) in localities near to residential areas, compared to localities with a large distance from residential areas (8%, 20/258). Sex and age of wild small mammals did not have effect on their positivity. Genotyping analysis revealed E. cuniculi genotype II in 22 samples and E. hellem genotype 1 A in one sample. Conclusion: This study brings new information on the molecular characterization of Encephalitozoon spp. isolated from wild small mammals trapped in two different areas (localities in near to residential areas and localities with a large distance from residential areas).

Citovat

BÁRTOVÁ, Eva, Jiřina MARKOVÁ, Alena ŽÁKOVSKÁ, Zuzana ČADKOVÁ a Marie BUDÍKOVÁ. Wildlife as Reservoirs of Encephalitozoon Cuniculi and Encephalitozoon Hellem and Molecular Genotyping of Encephalitozoon spp. in Small Mammals in the Czech Republic. Acta Parasitologica. Springer, 2024, 6 s. ISSN 1230-2821. Dostupné z: https://dx.doi.org/10.1007/s11686-024-00920-0.

@article{2440038,
   author = {Bártová, Eva and Marková, Jiřina and Žákovská, Alena and Čadková, Zuzana and Budíková, Marie},
   doi = {http://dx.doi.org/10.1007/s11686-024-00920-0},
   keywords = {Encephalitozoonosis; Wildlife; Urban area; Zoonosis; Genotyping},
   language = {eng},
   issn = {1230-2821},
   journal = {Acta Parasitologica},
   title = {Wildlife as Reservoirs of Encephalitozoon Cuniculi and Encephalitozoon Hellem and Molecular Genotyping of Encephalitozoon spp. in Small Mammals in the Czech Republic},
   url = {https://link.springer.com/article/10.1007/s11686-024-00920-0},
   year = {2024}
}

TY  - JOUR
ID  - 2440038
AU  - Bártová, Eva - Marková, Jiřina - Žákovská, Alena - Čadková, Zuzana - Budíková, Marie
PY  - 2024
TI  - Wildlife as Reservoirs of Encephalitozoon Cuniculi and Encephalitozoon Hellem and Molecular Genotyping of Encephalitozoon spp. in Small Mammals in the Czech Republic
JF  - Acta Parasitologica
PB  - Springer
SN  - 12302821
KW  - Encephalitozoonosis
KW  - Wildlife
KW  - Urban area
KW  - Zoonosis
KW  - Genotyping
UR  - https://link.springer.com/article/10.1007/s11686-024-00920-0
N2  - Purpose: Parasites of genus Encephalitozoon are well known pathogens of domestic animals however less attention was paid to its spread among wildlife that can play an important role of reservoir of infection. The aim of the study was to conduct molecular detection and genotype characterization of Encephalitozoon spp. in wild small mammals trapped in localities both near to and at a large distance from residential areas. Methods: In total, 300 wild small mammals (274 Rodentia and 26 Eulipotyphla) were trapped in 41 localities of the Czech Republic and tested by nested PCR for Encephalitozoon spp. Results: The DNA of Encephalitozoon spp. was proved in tissues (brain or liver) of 11% (32/300) of animals. There was a statistically significant difference (p < 0.001) in positivity among animal species with the most infected species Micromys minutus (50%, 4/8) and Myodes glareolus (17%, 9/53). There was also statistically significant difference (p < 0.001) between localities with the higher positivity (29%, 12/42) in localities near to residential areas, compared to localities with a large distance from residential areas (8%, 20/258). Sex and age of wild small mammals did not have effect on their positivity. Genotyping analysis revealed E. cuniculi genotype II in 22 samples and E. hellem genotype 1 A in one sample. Conclusion: This study brings new information on the molecular characterization of Encephalitozoon spp. isolated from wild small mammals trapped in two different areas (localities in near to residential areas and localities with a large distance from residential areas).
ER  -

BÁRTOVÁ, Eva, Jiřina MARKOVÁ, Alena ŽÁKOVSKÁ, Zuzana ČADKOVÁ a Marie BUDÍKOVÁ. Wildlife as Reservoirs of Encephalitozoon Cuniculi and Encephalitozoon Hellem and Molecular Genotyping of Encephalitozoon spp. in Small Mammals in the Czech Republic. \textit{Acta Parasitologica}. Springer, 2024, 6 s. ISSN~1230-2821. Dostupné z: https://dx.doi.org/10.1007/s11686-024-00920-0.

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